Mag-Bind® FFPE DNA/RNA 96 Kit

$0.00$2,261.50

Sequential isolation of both DNA and RNA from the same FFPE sample using magnetic beads

  • Xylene-free protocol
  • Extraction of both DNA and RNA in separate eluates from the same FFPE sample
  • No splitting of samples
  • Magnetic bead-based purification
  • Automation friendly

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

Mag-Bind®  FFPE DNA/RNA 96 Kit is designed for sequentially isolating DNA and RNA from the same formalin-fixed, paraffin-embedded (FFPE) tissue sample. The protocol utilizes non-toxic mineral oil in combination with heat for efficient deparaffinization of the FFPE sample, eliminating the use of hazardous xylene.

The specially formulated buffers reverse cross-linking without the need for overnight digestion, resulting in high-yielding, high-quality nucleic acids. The isolation protocol allows for the extraction of DNA and RNA in separate eluates from the same sample for a comprehensive analysis of both nucleic acids. Purified DNA and RNA are suitable for a variety of downstream applications, including SNP analysis, sequencing, and genotyping.

Mag-Bind® system is fully automatable on Hamilton Microlab® STAR™, Tecan Freedom Evo®, Thermo Fisher Scientific KingFisher® Flex Purification System, and other open-ended workstations.

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Downstream ApplicationsNGS, PCR, qPCR, real-time RT-PCR, microarray, microRNA analysis
Elution Volume50-200 µL
Starting MaterialFFPE tissue
Starting Amount< 3 FFPE sections of 10 μm thickness each
ThroughputUp to 96
Processing ModeAutomated, Manual
TechnologyMagnetic beads
ITEMAVAILABLE SEPARATELY
FDR Buffer– – –
Proteinase K SolutionView Product
MB4 Buffer– – –
Mag-Bind® Particles CH– – –
RMP Buffer– – –
Elution BufferView Product
DNase Digestion Buffer– – –
Mag-Bind® DNase I– – –
PHM Buffer– – –
Nuclease-free WaterView Product

High Yield RNA & DNA

Figure 1. Average DNA (A) and RNA (B) yields from FFPE tumor samples. Genomic DNA (A) and RNA (B) was sequentially isolated from the same 1 × 10 μm section of the FFPE tumor tissue sample (n=3) using Omega Bio-tek’s Mag-Bind® FFPE DNA/RNA 96 Kit and comparable kits from Company T and Company Q following manufacturer’s recommended protocols. Purified DNA and RNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system as well as Promega’s QuantiFluor® dsDNA and RNA system.

High Quality DNA & RNA

Figure 2. Purity of DNA (A) and RNA (B) isolated using different manufacturer’s kits was analyzed through spectrophotometry focusing on A260/A280 and A260/A230 ratios.

PCR Analysis

Figure 3. Purity of DNA (A) and RNA (B) isolated using different manufacturer’s kits was analyzed through spectrophotometry focusing on A260/A280 and A260/A230 ratios.

DV200 Analysis

Table 1. Average DV200 value (percentage of fragments >200 nt) of RNA purified using different kits analyzed on Agilent’s TapeStation® 2200.

Cq Value Comparison

Table 3. ΔCq values of DNA extracted from FFPE and non-FFPE colorectal tumor tissue samples using kits from different manufacturers.

Aligned Reads

Table 3. Omega Bio-tek’s Mag-Bind FFPE DNA/RNA 96 Kit showed a higher percentage of aligned reads compared to Kits from Company T and Company Q across breast, lung, and colorectal tumor tissue types

Small Variant Calling

Figure 4. Small Variant Calling Analysis on DNA from FFPE colorectal tumor tissue sample extracted from different kits compared to fresh frozen control.

Product Information

NameDocumentLanguagesLink
Quick GuideProduct ManualEnglish
Protocol ManualProduct ManualEnglish
Mag-Bind® FFPE DNA/RNA 96 KitProduct LiteratureEnglish
Comparison Analysis of Yield, Quality and Next Generation Sequencing from FFPE Samples Using Three Total Nucleic Acid Isolation KitsApplication NoteEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DNase I Digestion BufferGHSEnglishenglishghs
DNase I Digestion BufferGHSSpanishspanishghs
DNase I Digestion BufferREACHEnglishenglishreach
DNase I Digestion BufferREACHDanishdanishreach
DNase I Digestion BufferREACHFinnishfinnishreach
DNase I Digestion BufferREACHFrenchfrenchreach
DNase I Digestion BufferREACHGermangermanreach
DNase I Digestion BufferREACHItalianitalianreach
DNase I Digestion BufferREACHNorwegiannorwegianreach
DNase I Digestion BufferREACHSpanishspanishreach
DNase I Digestion BufferREACHSwedishswedishreach
DNase I Digestion BufferWHMSEnglishenglishwhms
DNase I Digestion BufferWHMSFrenchfrenchwhms
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHGreekgreekreach
FDR BufferGHSEnglishenglishghs
FDR BufferGHSSpanishspanishghs
FDR BufferREACHEnglishenglishreach
FDR BufferREACHDanishdanishreach
FDR BufferREACHFinnishfinnishreach
FDR BufferREACHFrenchfrenchreach
FDR BufferREACHGermangermanreach
FDR BufferREACHItalianitalianreach
FDR BufferREACHNorwegiannorwegianreach
FDR BufferREACHSpanishspanishreach
FDR BufferREACHSwedishswedishreach
FDR BufferWHMSEnglishenglishwhms
FDR BufferWHMSFrenchfrenchwhms
Mag-Bind DNase IGHSEnglishenglishghs
Mag-Bind DNase IGHSSpanishspanishghs
Mag-Bind DNase IREACHEnglishenglishreach
Mag-Bind DNase IREACHDanishdanishreach
Mag-Bind DNase IREACHFinnishfinnishreach
Mag-Bind DNase IREACHFrenchfrenchreach
Mag-Bind DNase IREACHGermangermanreach
Mag-Bind DNase IREACHItalianitalianreach
Mag-Bind DNase IREACHNorwegiannorwegianreach
Mag-Bind DNase IREACHSpanishspanishreach
Mag-Bind DNase IREACHSwedishswedishreach
Mag-Bind DNase IWHMSEnglishenglishwhms
Mag-Bind DNase IWHMSFrenchfrenchwhms
Mag-Bind Particles CHGHSEnglishenglishghs
Mag-Bind Particles CHGHSSpanishspanishghs
Mag-Bind Particles CHREACHEnglishenglishreach
Mag-Bind Particles CHREACHDanishdanishreach
Mag-Bind Particles CHREACHFinnishfinnishreach
Mag-Bind Particles CHREACHFrenchfrenchreach
Mag-Bind Particles CHREACHGermangermanreach
Mag-Bind Particles CHREACHItalianitalianreach
Mag-Bind Particles CHREACHNorwegiannorwegianreach
Mag-Bind Particles CHREACHSpanishspanishreach
Mag-Bind Particles CHREACHSwedishswedishreach
Mag-Bind Particles CHWHMSEnglishenglishwhms
Mag-Bind Particles CHWHMSFrenchfrenchwhms
Mag-Bind Particles CHREACHDutchdutchreach
Mag-Bind Particles CHREACHHungarianhungarianreach
Mag-Bind Particles CHREACHPortugueseportuguesereach
Mag-Bind Particles CHREACHGreekgreekreach
MB4 BufferGHSEnglishenglishghs
MB4 BufferGHSSpanishspanishghs
MB4 BufferREACHEnglishenglishreach
MB4 BufferREACHDanishdanishreach
MB4 BufferREACHFinnishfinnishreach
MB4 BufferREACHFrenchfrenchreach
MB4 BufferREACHGermangermanreach
MB4 BufferREACHItalianitalianreach
MB4 BufferREACHNorwegiannorwegianreach
MB4 BufferREACHSpanishspanishreach
MB4 BufferREACHSwedishswedishreach
MB4 BufferWHMSEnglishenglishwhms
MB4 BufferWHMSFrenchfrenchwhms
Nuclease-free WaterGHSEnglishenglishghs
Nuclease-free WaterGHSSpanishspanishghs
Nuclease-free WaterREACHEnglishenglishreach
Nuclease-free WaterREACHDanishdanishreach
Nuclease-free WaterREACHFinnishfinnishreach
Nuclease-free WaterREACHFrenchfrenchreach
Nuclease-free WaterREACHGermangermanreach
Nuclease-free WaterREACHItalianitalianreach
Nuclease-free WaterREACHNorwegiannorwegianreach
Nuclease-free WaterREACHSpanishspanishreach
Nuclease-free WaterREACHSwedishswedishreach
Nuclease-free WaterWHMSEnglishenglishwhms
Nuclease-free WaterWHMSFrenchfrenchwhms
Nuclease-free WaterREACHDutchdutchreach
Nuclease-free WaterREACHHungarianhungarianreach
Nuclease-free WaterREACHPortugueseportuguesereach
Nuclease-free WaterREACHGreekgreekreach
PHM BufferGHSEnglishenglishghs
PHM BufferGHSSpanishspanishghs
PHM BufferREACHEnglishenglishreach
PHM BufferREACHDanishdanishreach
PHM BufferREACHFinnishfinnishreach
PHM BufferREACHFrenchfrenchreach
PHM BufferREACHGermangermanreach
PHM BufferREACHItalianitalianreach
PHM BufferREACHNorwegiannorwegianreach
PHM BufferREACHSpanishspanishreach
PHM BufferREACHSwedishswedishreach
PHM BufferWHMSEnglishenglishwhms
PHM BufferWHMSFrenchfrenchwhms
Proteinase K SolutionGHSEnglishenglishghs
Proteinase K SolutionGHSSpanishspanishghs
Proteinase K SolutionREACHEnglishenglishreach
Proteinase K SolutionREACHDanishdanishreach
Proteinase K SolutionREACHFinnishfinnishreach
Proteinase K SolutionREACHFrenchfrenchreach
Proteinase K SolutionREACHGermangermanreach
Proteinase K SolutionREACHItalianitalianreach
Proteinase K SolutionREACHNorwegiannorwegianreach
Proteinase K SolutionREACHSpanishspanishreach
Proteinase K SolutionREACHSwedishswedishreach
Proteinase K SolutionWHMSEnglishenglishwhms
Proteinase K SolutionWHMSFrenchfrenchwhms
Proteinase K SolutionREACHDutchdutchreach
Proteinase K SolutionREACHHungarianhungarianreach
Proteinase K SolutionREACHPortugueseportuguesereach
Proteinase K SolutionREACHGreekgreekreach
RMP BufferGHSEnglishenglishghs
RMP BufferGHSSpanishspanishghs
RMP BufferREACHEnglishenglishreach
RMP BufferREACHDanishdanishreach
RMP BufferREACHFinnishfinnishreach
RMP BufferREACHFrenchfrenchreach
RMP BufferREACHGermangermanreach
RMP BufferREACHItalianitalianreach
RMP BufferREACHNorwegiannorwegianreach
RMP BufferREACHSpanishspanishreach
RMP BufferREACHSwedishswedishreach
RMP BufferWHMSEnglishenglishwhms
RMP BufferWHMSFrenchfrenchwhms
RMP BufferREACHDutchdutchreach
RMP BufferREACHHungarianhungarianreach
RMP BufferREACHPortugueseportuguesereach
RMP BufferREACHGreekgreekreach
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