Price range: $0.00 through $1,592.90
Isolate DNA from soil and water samples using magnetic beads
All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.
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Scripts available for all Mag-Bind® Kits for most open liquid handlers and common magnetic processors including the MagBinder® Fit24. Visit Automated Solutions for more information.
The Mag-Bind® Environmental DNA 96 Kit allows rapid and reliable isolation of high-quality DNA from soil and water samples. The Mag-Bind® Environmental DNA 96 Kit can isolate microbial DNA from yeast, fungi, and gram-positive or negative bacteria. Up to 96 one hundred mg of soil samples can be processed in 120 minutes using automated liquid handlers or magnetic processors. Omega Bio-tek’s unique cHTR Reagent effectively removes humic acid and other PCR inhibitors, allowing for purified DNA to be suitable for PCR, 16S sequencing, Whole Genome Sequencing, and Next Generation Sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.
Protocols are available for the following automated platforms:
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Application | qPCR, PCR, Next Generation Sequencing |
| Starting material | Up to 250 mg soil or 1 water filter |
| Target Nucleic Acid | Gram Negative and positive bacteria, yeast, fungal |
| Processing mode | Automated or Manual |
| Throughput | 96 samples |
| Processing time | 120 minutes |
| DNA binding technology | Magnetic Beads |
| Yield | Dependent on sample biomass |
| ITEM | AVAILABLE SEPARATELY |
|---|---|
| E-Z 96 Disruptor Plate C Plus | View Product |
| 96-well Racked Microtubes | — |
| Caps for Racked Microtubes | — |
| SLX-Mlus Buffer | View Product |
| DS Buffer | Call for Pricing |
| P2 Buffer | View Product |
| XP1 Buffer | — |
| VHB Buffer | View Product |
| SPM Wash Buffer | View Product |
| Elution Buffer | View Product |
| Mag-Bind® Particles RQ | Call for Pricing |
| cHTR Reagent | — |
| RNase A | View Product |
| Soil Protocol | Yield (µg) | A260/A280 | A260/A230 |
|---|---|---|---|
| Omega Bio-tek | 3.16 | 1.86 | 1.11 |
| 3.15 | 1.84 | 1.21 | |
| 2.92 | 1.9 | 1.08 | |
| 2.90 | 1.85 | 1.2 | |
| Company Q | 1.40 | 1.33 | 0.58 |
| 1.40 | 1.4 | 0.65 |
Table 1. Outdoor soil (250mg) was spiked with an approximately 1mL culture of Bacillus subtilis and DNA was extracted using Mag-Bind® Environmental kit (Omega Bio-tek) and Soil DNA Isolation kit (Company Q) following manufacturer’s recommended protocols. The isolations were carried out in quadruplicate for the Omega kit and in duplicate for the competitor’s kit. DNA was quantified using Thermo Scientific’s NanoDrop® 2000c. The Kit from Omega Bio-tek exhibited higher yields of high-quality DNA than the kit from Company Q.
| Water Protocol | Yield (µg) | A260/A280 | A260/A230 |
|---|---|---|---|
| Omega Bio-tek | 5.71 | 1.77 | 1.84 |
| 6.80 | 1.78 | 1.88 | |
| 5.98 | 1.8 | 1.84 | |
| 5.78 | 1.8 | 1.9 | |
| Company Q | 2.90 | 1.86 | 1.36 |
| 3.51 | 1.89 | 1.33 | |
| 3.02 | 1.87 | 1.09 | |
| 3.38 | 1.87 | 1.95 |
Table 2. Pond water (100mL) was spiked with approximately 1mL culture each of B. subtilis and E. coli harboring pGEM plasmid and was filtered through Sterivex (Millipore SVGPL10RC) filters. Upon filtration, DNA was extracted from these filters in quadruplicate following the manufacturer’s recommended protocols. DNA was quantified using Thermo Scientific’s NanoDrop® 2000c. The Kit from Omega Bio-tek exhibited higher yields of high-quality DNA than the kit from Company Q.
Figure 1. DNA was analyzed by performing real-time PCR using B. subtilis specific primers on undiluted and 10- fold diluted DNA isolated from soil extraction protocol and on 10-fold and 100-fold diluted DNA isolated from water extraction protocol. Briefly, a qPCR reaction was set up to a total volume of 20 µL using Agilent’s Brilliant III 2X SYBR® as the master mix and 2 µL of template DNA at appropriate dilutions amplified with suitable primers following a standard protocol on the ABI 7900. Undiluted DNA from soil extracted with Company Q’s kit showed no amplification, indicating inhibitor carryover, while Omega Bio-tek’s kit enabled robust amplification, demonstrating effective inhibitor removal for both sample types. At 10× dilution, Omega yielded Ct values 1.3 cycles lower (~2.5× higher DNA yield), consistent with NanoDrop® quantification.
