Mag-Bind® Environmental DNA 96 Kit


Isolate DNA from soil and water samples using magnetic beads

  • Ceramic Beads pre-aliquoted in a convenient 96-well format
  • Unique inhibitor removal Reagent
  • Automation Friendly

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

The Mag-Bind® Environmental DNA 96 Kit allows rapid and reliable isolation of high-quality DNA from soil and water samples. The Mag-Bind® Environmental DNA 96 Kit can isolate microbial DNA from yeast, fungi, and gram-positive or negative bacteria. Up to 96 one hundred mg of soil samples can be processed in 120 minutes using automated liquid handlers or magnetic processors. Omega Bio-tek’s unique cHTR Reagent effectively removes humic acid and other PCR inhibitors, allowing for purified DNA to be suitable for PCR, 16S sequencing, Whole Genome Sequencing, and Next Generation Sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.

Protocols are available for the following automated platforms:

  • Hamilton Microlab® STAR
  • Hamilton Microlab® NIMBUS
  • KingFisher™, BioSprint®, and MagMAX® 96

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationqPCR, PCR, Next Generation Sequencing
Starting materialUp to 250 mg soil or 1 water filter
Target Nucleic AcidGram Negative and positive bacteria, yeast, fungal
Processing modeAutomated or Manual
Throughput96 samples
Processing time120 minutes
DNA binding technologyMagnetic Beads
YieldDependent on sample biomass
E-Z 96 Disruptor Plate C PlusView Product
96-well Racked Microtubes
Caps for Racked Microtubes
SLX-Mlus BufferView Product
DS BufferCall for Pricing
P2 BufferView Product
XP1 Buffer
VHB BufferView Product
SPM Wash BufferView Product
Elution BufferView Product
Mag-Bind® Particles RQCall for Pricing
cHTR Reagent
RNase AView Product

Yield and Quality

Figure 1.  Outdoor soil (250mg) was spiked with an approximately 1mL culture of Bacillus subtilis and DNA was extracted using Mag-Bind® Environmental kit (Omega Bio-tek) and Soil DNA Isolation kit (Company Q) following manufacturer’s recommended protocols. The isolations were carried out in quadruplicate for the Omega kit and in duplicate for the competitor’s kit. DNA was quantified using Thermo Scientific’s NanoDrop® 2000c. The quality of the purified DNA was analyzed by performing real-time PCR using Bacillus subtilis specific primers on undiluted and 10-fold diluted DNA isolated from soil extraction protocol.

qPCR Comparison

Figure 2.  Pond water (100mL) was spiked with approximately 1mL culture each of B. subtilis and E. coli harboring pGEM plasmid and was filtered through Sterivex (Millipore SVGPL10RC) filters. Upon filtration, DNA was extracted from these filters in quadruplicate following the manufacturer’s recommended protocols. DNA was quantified using Thermo Scientific’s NanoDrop® 2000c. The quality of the purified DNA was analyzed by performing real-time PCR using Bacillus subtilis specific primers on 10-fold diluted and 100-fold diluted DNA isolated from water extraction protocol.

Product Information

Protocol ManualProduct ManualEnglish
Magnetic bead-based solution for DNA extraction from environmental samples using Mag-Bind® Environmental DNA 96 Kit from Omega Bio-tekApplication NoteEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
cHTR ReagentGHSEnglishenglishghs
cHTR ReagentGHSSpanishspanishghs
cHTR ReagentREACHEnglishenglishreach
cHTR ReagentREACHDanishdanishreach
cHTR ReagentREACHFinnishfinnishreach
cHTR ReagentREACHFrenchfrenchreach
cHTR ReagentREACHGermangermanreach
cHTR ReagentREACHItalianitalianreach
cHTR ReagentREACHNorwegiannorwegianreach
cHTR ReagentREACHSpanishspanishreach
cHTR ReagentREACHSwedishswedishreach
cHTR ReagentWHMSEnglishenglishwhms
cHTR ReagentWHMSFrenchfrenchwhms
DS BufferGHSEnglishenglishghs
DS BufferGHSSpanishspanishghs
DS BufferREACHEnglishenglishreach
DS BufferREACHDanishdanishreach
DS BufferREACHFinnishfinnishreach
DS BufferREACHFrenchfrenchreach
DS BufferREACHGermangermanreach
DS BufferREACHItalianitalianreach
DS BufferREACHNorwegiannorwegianreach
DS BufferREACHSpanishspanishreach
DS BufferREACHSwedishswedishreach
DS BufferWHMSEnglishenglishwhms
DS BufferWHMSFrenchfrenchwhms
DS BufferREACHDutchdutchreach
DS BufferREACHHungarianhungarianreach
DS BufferREACHPortugueseportuguesereach
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
Mag-Bind Particles RQGHSEnglishenglishghs
Mag-Bind Particles RQGHSSpanishspanishghs
Mag-Bind Particles RQREACHEnglishenglishreach
Mag-Bind Particles RQREACHDanishdanishreach
Mag-Bind Particles RQREACHFinnishfinnishreach
Mag-Bind Particles RQREACHFrenchfrenchreach
Mag-Bind Particles RQREACHGermangermanreach
Mag-Bind Particles RQREACHItalianitalianreach
Mag-Bind Particles RQREACHNorwegiannorwegianreach
Mag-Bind Particles RQREACHSpanishspanishreach
Mag-Bind Particles RQREACHSwedishswedishreach
Mag-Bind Particles RQWHMSEnglishenglishwhms
Mag-Bind Particles RQWHMSFrenchfrenchwhms
Mag-Bind Particles RQREACHDutchdutchreach
Mag-Bind Particles RQREACHHungarianhungarianreach
Mag-Bind Particles RQREACHPortugueseportuguesereach
P2 BufferGHSEnglishenglishghs
P2 BufferGHSSpanishspanishghs
P2 BufferREACHEnglishenglishreach
P2 BufferREACHDanishdanishreach
P2 BufferREACHFinnishfinnishreach
P2 BufferREACHFrenchfrenchreach
P2 BufferREACHGermangermanreach
P2 BufferREACHItalianitalianreach
P2 BufferREACHNorwegiannorwegianreach
P2 BufferREACHSpanishspanishreach
P2 BufferREACHSwedishswedishreach
P2 BufferWHMSEnglishenglishwhms
P2 BufferWHMSFrenchfrenchwhms
RNase AGHSEnglishenglishghs
RNase AGHSSpanishspanishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFinnishfinnishreach
RNase AREACHFrenchfrenchreach
RNase AREACHGermangermanreach
RNase AREACHItalianitalianreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHSpanishspanishreach
RNase AREACHSwedishswedishreach
RNase AWHMSEnglishenglishwhms
RNase AWHMSFrenchfrenchwhms
SLX M1us BufferGHSEnglishenglishghs
SLX M1us BufferGHSSpanishspanishghs
SLX M1us BufferREACHEnglishenglishreach
SLX M1us BufferREACHDanishdanishreach
SLX M1us BufferREACHFinnishfinnishreach
SLX M1us BufferREACHFrenchfrenchreach
SLX M1us BufferREACHGermangermanreach
SLX M1us BufferREACHItalianitalianreach
SLX M1us BufferREACHNorwegiannorwegianreach
SLX M1us BufferREACHSpanishspanishreach
SLX M1us BufferREACHSwedishswedishreach
SLX M1us BufferWHMSEnglishenglishwhms
SLX M1us BufferWHMSFrenchfrenchwhms
SPM BufferGHSEnglishenglishghs
SPM BufferGHSSpanishspanishghs
SPM BufferREACHEnglishenglishreach
SPM BufferREACHDanishdanishreach
SPM BufferREACHFinnishfinnishreach
SPM BufferREACHFrenchfrenchreach
SPM BufferREACHGermangermanreach
SPM BufferREACHItalianitalianreach
SPM BufferREACHNorwegiannorwegianreach
SPM BufferREACHSpanishspanishreach
SPM BufferREACHSwedishswedishreach
SPM BufferWHMSEnglishenglishwhms
SPM BufferWHMSFrenchfrenchwhms
SPM BufferREACHDutchdutchreach
SPM BufferREACHHungarianhungarianreach
SPM BufferREACHPortugueseportuguesereach
VHB BufferGHSEnglishenglishghs
VHB BufferGHSSpanishspanishghs
VHB BufferREACHEnglishenglishreach
VHB BufferREACHDanishdanishreach
VHB BufferREACHFinnishfinnishreach
VHB BufferREACHFrenchfrenchreach
VHB BufferREACHGermangermanreach
VHB BufferREACHItalianitalianreach
VHB BufferREACHNorwegiannorwegianreach
VHB BufferREACHSpanishspanishreach
VHB BufferREACHSwedishswedishreach
VHB BufferWHMSEnglishenglishwhms
VHB BufferWHMSFrenchfrenchwhms
VHB BufferREACHDutchdutchreach
VHB BufferREACHHungarianhungarianreach
VHB BufferREACHPortugueseportuguesereach
XP1 BufferGHSEnglishenglishghs
XP1 BufferGHSSpanishspanishghs
XP1 BufferREACHEnglishenglishreach
XP1 BufferREACHDanishdanishreach
XP1 BufferREACHFinnishfinnishreach
XP1 BufferREACHFrenchfrenchreach
XP1 BufferREACHGermangermanreach
XP1 BufferREACHItalianitalianreach
XP1 BufferREACHNorwegiannorwegianreach
XP1 BufferREACHSpanishspanishreach
XP1 BufferREACHSwedishswedishreach
XP1 BufferWHMSEnglishenglishwhms
XP1 BufferWHMSFrenchfrenchwhms
DS BufferREACHGreekgreekreach
Elution BufferREACHGreekgreekreach
Mag-Bind Particles RQREACHGreekgreekreach
SPM BufferREACHGreekgreekreach
VHB BufferREACHGreekgreekreach
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