E-Z 96™ FastFilter Plasmid Kit


Isolate plasmid DNA from bacterial cultures in 96-well plate format

  • Clear lysate with E-Z 96™ Lysate Clearance Plate
  • No resin, slurries, or alcohol precipitation
  • High-purity plasmid DNA in less than 1 hour
  • Reproducible yields of molecular biology grade plasmid DNA
  • Fast, easy and convenient protocols

The E-Z 96™ FastFilter Plasmid Kit is optimized for the parallel preparation of up to 96 plasmid minipreps in 1 hour. The Lysate Clearance Plate replaces the time-consuming centrifugation involved in clearing of bacterial alkaline lysates. This kit contains all of the components needed for plasmid DNA purification in a 96-well format. Purified plasmid DNA is suitable for the most sensitive downstream applications, such as routine screening or automated DNA sequencing. The E-Z 96™ Fastfilter Plasmid Kits newly designed 96-well DNA plates are ideal for use with automated liquid handlers.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationCloning; sanger sequencing etc.
Starting materialPer well: 1.0-1.5 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid typeHigh-copy, low-copy, cosmid DNA
Processing mode96-well, centrifugation or vacuum
Throughput96 samples per run
DNA binding technologySilica 96-well plate
Lysate clearance methodCentrifugation or vacuum with lysate clearnce plate
Processing time<1 hour
Yield15-25 µg for high copy-number; 0.1-5 µg for low copy-number
Special noteLysate clearance plate for fast processing

Figure 1.  Plasmid DNA purified using the E-Z 96™ FastFilter Plasmid Kit. DNA was isolated from 48 1 mL bacterial cultures frown overnight in LB culture using the E-Z 96™ FastFilter Plasmid Kit and eluted in 150 µL elution buffer. Plasmid DNA (10% of total purified DNA) was analyzed on 1.0% agarose gel. Lanes 1-48 represent plasmid DNA from 48 different bacterial cultures.

Product Information

Protocol ManualProduct ManualEnglish
E-Z 96™ FastFilter Plasmid DNA KitProduct LiteratureEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferWHMSFrenchfrenchwhms
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHEnglishenglishreach
Elution BufferREACHGreekgreekreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHDutchdutchreach
Elution BufferWHMSFrenchfrenchwhms
Elution BufferWHMSEnglishenglishwhms
Elution BufferREACHSwedishswedishreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHItalianitalianreach
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHGermangermanreach
Elution BufferREACHFinnishfinnishreach
Endo-Free Elution BufferGHSEnglishenglishghs
Endo-Free Elution BufferREACHDanishdanishreach
Endo-Free Elution BufferREACHEnglishenglishreach
Endo-Free Elution BufferGHSSpanishspanishghs
HBC BufferGHSEnglishenglishghs
HBC BufferWHMSFrenchfrenchwhms
HBC BufferWHMSEnglishenglishwhms
HBC BufferREACHSwedishswedishreach
HBC BufferREACHSpanishspanishreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHGermangermanreach
HBC BufferGHSSpanishspanishghs
HBC BufferREACHEnglishenglishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHFinnishfinnishreach
RNase AGHSSpanishspanishghs
RNase AWHMSFrenchfrenchwhms
RNase AWHMSEnglishenglishwhms
RNase AREACHSwedishswedishreach
RNase AREACHSpanishspanishreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHGermangermanreach
RNase AGHSEnglishenglishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFrenchfrenchreach
RNase AREACHItalianitalianreach
RNase AREACHFinnishfinnishreach
Solution IGHSSpanishspanishghs
Solution IWHMSFrenchfrenchwhms
Solution IWHMSEnglishenglishwhms
Solution IREACHSwedishswedishreach
Solution IREACHSpanishspanishreach
Solution IREACHNorwegiannorwegianreach
Solution IREACHGermangermanreach
Solution IGHSEnglishenglishghs
Solution IREACHEnglishenglishreach
Solution IREACHDanishdanishreach
Solution IREACHFrenchfrenchreach
Solution IREACHFinnishfinnishreach
Solution IREACHItalianitalianreach
Solution IIREACHGermangermanreach
Solution IIREACHFrenchfrenchreach
Solution IIGHSSpanishspanishghs
Solution IIREACHItalianitalianreach
Solution IIREACHEnglishenglishreach
Solution IIREACHNorwegiannorwegianreach
Solution IIREACHSpanishspanishreach
Solution IIREACHDanishdanishreach
Solution IIREACHSwedishswedishreach
Solution IIREACHFinnishfinnishreach
Solution IIWHMSEnglishenglishwhms
Solution IIGHSEnglishenglishghs
Solution IIWHMSFrenchfrenchwhms
Solution IIIGHSEnglishenglishghs
Solution IIIGHSSpanishspanishghs
Solution IIIREACHEnglishenglishreach
Solution IIIREACHDanishdanishreach
Solution IIIREACHFinnishfinnishreach
Solution IIIREACHFrenchfrenchreach
Solution IIIREACHGermangermanreach
Solution IIIREACHItalianitalianreach
Solution IIIREACHNorwegiannorwegianreach
Solution IIIREACHSpanishspanishreach
Solution IIIREACHSwedishswedishreach
Solution IIIWHMSEnglishenglishwhms
Solution IIIWHMSFrenchfrenchwhms
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