E-Z 96™ Total RNA Kit


Isolate total RNA from cultured cells or soft tissues using 96-well plate format.

The E-Z 96™ Total RNA Kit is designed for isolation of total cellular RNA from up to 5 x 105 cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilizing HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High-quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96™ Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationPCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume50-75 μL
Starting MaterialCulture cells
Starting Amountup to 5×105 cells
Processing ModeManual, centrifugation/vacuum
Throughput96 samples per run
RNA Binding technologySilica membrane 96-well

Figure 1. Purified total RNA from 10 mg chicken liver was isolated with the E-Z 96™ Total RNA Kit. Total RNA (500 ng/lane) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA.

Product Information

Document NameLanguagesLink
Protocol ManualEnglish
E-Z 96® Total RNA KitEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
Nuclease-free WaterGHSEnglishenglishghs
Nuclease-free WaterGHSSpanishspanishghs
Nuclease-free WaterREACHGermangermanreach
Nuclease-free WaterREACHFrenchfrenchreach
Nuclease-free WaterREACHDanishdanishreach
Nuclease-free WaterREACHEnglishenglishreach
Nuclease-free WaterREACHFinnishfinnishreach
Nuclease-free WaterREACHItalianitalianreach
Nuclease-free WaterREACHNorwegiannorwegianreach
Nuclease-free WaterREACHSpanishspanishreach
Nuclease-free WaterREACHSwedishswedishreach
Nuclease-free WaterWHMSEnglishenglishwhms
Nuclease-free WaterWHMSFrenchfrenchwhms
Nuclease-free WaterREACHDutchdutchreach
Nuclease-free WaterREACHHungarianhungarianreach
Nuclease-free WaterREACHPortugueseportuguesereach
RNA Wash Buffer IIGHSEnglishenglishghs
RNA Wash Buffer IIGHSSpanishspanishghs
RNA Wash Buffer IIREACHEnglishenglishreach
RNA Wash Buffer IIREACHDanishdanishreach
RNA Wash Buffer IIREACHFinnishfinnishreach
RNA Wash Buffer IIREACHFrenchfrenchreach
RNA Wash Buffer IIREACHGermangermanreach
RNA Wash Buffer IIREACHItalianitalianreach
RNA Wash Buffer IIREACHNorwegiannorwegianreach
RNA Wash Buffer IIREACHSpanishspanishreach
RNA Wash Buffer IIREACHSwedishswedishreach
RNA Wash Buffer IIWHMSFrenchfrenchwhms
RNA Wash Buffer IIWHMSEnglishenglishwhms
RWF BufferGHSEnglishenglishghs
RWF BufferGHSSpanishspanishghs
RWF BufferREACHEnglishenglishreach
RWF BufferREACHDanishdanishreach
RWF BufferREACHFinnishfinnishreach
RWF BufferREACHFrenchfrenchreach
RWF BufferREACHGermangermanreach
RWF BufferREACHItalianitalianreach
RWF BufferREACHNorwegiannorwegianreach
RWF BufferREACHSpanishspanishreach
RWF BufferREACHSwedishswedishreach
RWF BufferWHMSEnglishenglishwhms
RWF BufferWHMSFrenchfrenchwhms
TRK Lysis BufferGHSEnglishenglishghs
TRK Lysis BufferGHSSpanishspanishghs
TRK Lysis BufferREACHEnglishenglishreach
TRK Lysis BufferREACHDanishdanishreach
TRK Lysis BufferREACHFinnishfinnishreach
TRK Lysis BufferREACHFrenchfrenchreach
TRK Lysis BufferREACHGermangermanreach
TRK Lysis BufferREACHItalianitalianreach
TRK Lysis BufferREACHNorwegiannorwegianreach
TRK Lysis BufferREACHSpanishspanishreach
TRK Lysis BufferREACHSwedishswedishreach
TRK Lysis BufferWHMSEnglishenglishwhms
TRK Lysis BufferWHMSFrenchfrenchwhms
Nuclease-free WaterREACHGreekgreekreach
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