E.Z.N.A.® Bacterial RNA Kit


Isolate high-quality RNA from gram-positive or gram-negative bacterial species using silica spin columns.

The E.Z.N.A.® Bacterial RNA Kit is designed for the isolation of high-quality total RNA from a variety of bacterial strains. Up to 1 x 109 log-phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridization techniques.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationPCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume50-100 μL
Starting MaterialBacteria
Starting AmountUp to 3 mL culture, 1 x 109 cells
RNA Yieldup to 100 µg
Processing ModeManual, centrifugation/vacuum
Throughput1 – 24
RNA Binding TechnologySilica Mini spin column
Binding Capacity100 µg
HiBind® RNA Mini Columns
2 mL Collection TubesView Product
OTRK Lysis Buffer
RNA Wash Buffer IView Product
RNA Wash Buffer IIView Product
Nuclease-free Water
Glass Beads S

Yield and Quality

Figure 1. Total RNA purified using the E.Z.N.A.® Bacterial RNA Kit. RNA was extracted from 2 mL bacterial samples as follows: sample 1 from Pseudomonas arginosa, sample 2 from Enterococcus faecalis, and sample 3 from Staphylococcus aureus. Total RNA (10% of total purified RNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA. M: CL5000 DNA Marker.

Yield and Quality from E. coli

Figure 2. Total RNA purified using the E.Z.N.A.® Bacterial RNA Kit. RNA was extracted from lag phase E. coli cultures as follows: sample 1 from 0.5 mL culture, sample 2 from 1.0 mL culture, sample 3 from 2 mL culture, and sample 4 from 3 mL culture. Lanes 1-4 represent the first 100 µL elution. Total RNA (50% of total purified RNA) was analyzed on a denaturing formaldehyde agarose gel to demonstrate yield and quality of the RNA. M: CL5000 DNA Marker.

Product Manuals

Protocol Manual
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Product Information

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Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
Nuclease-free WaterREACHGreekgreekreach
Nuclease-free WaterREACHPortugueseportuguesereach
Nuclease-free WaterREACHHungarianhungarianreach
Nuclease-free WaterREACHDutchdutchreach
Nuclease-free WaterWHMSFrenchfrenchwhms
Nuclease-free WaterWHMSEnglishenglishwhms
Nuclease-free WaterREACHSwedishswedishreach
Nuclease-free WaterREACHSpanishspanishreach
Nuclease-free WaterREACHItalianitalianreach
Nuclease-free WaterGHSEnglishenglishghs
Nuclease-free WaterGHSSpanishspanishghs
Nuclease-free WaterREACHEnglishenglishreach
Nuclease-free WaterREACHDanishdanishreach
Nuclease-free WaterREACHFrenchfrenchreach
Nuclease-free WaterREACHNorwegiannorwegianreach
Nuclease-free WaterREACHGermangermanreach
Nuclease-free WaterREACHFinnishfinnishreach
OTRK BufferREACHEnglishenglishreach
OTRK BufferGHSEnglishenglishghs
OTRK BufferWHMSFrenchfrenchwhms
OTRK BufferWHMSEnglishenglishwhms
OTRK BufferREACHSwedishswedishreach
OTRK BufferREACHSpanishspanishreach
OTRK BufferREACHItalianitalianreach
OTRK BufferGHSSpanishspanishghs
OTRK BufferREACHDanishdanishreach
OTRK BufferREACHFinnishfinnishreach
OTRK BufferREACHFrenchfrenchreach
OTRK BufferREACHNorwegiannorwegianreach
OTRK BufferREACHGermangermanreach
RNA Wash Buffer IGHSEnglishenglishghs
RNA Wash Buffer IWHMSFrenchfrenchwhms
RNA Wash Buffer IWHMSEnglishenglishwhms
RNA Wash Buffer IREACHSwedishswedishreach
RNA Wash Buffer IREACHSpanishspanishreach
RNA Wash Buffer IREACHNorwegiannorwegianreach
RNA Wash Buffer IREACHGermangermanreach
RNA Wash Buffer IGHSSpanishspanishghs
RNA Wash Buffer IREACHEnglishenglishreach
RNA Wash Buffer IREACHDanishdanishreach
RNA Wash Buffer IREACHFinnishfinnishreach
RNA Wash Buffer IREACHFrenchfrenchreach
RNA Wash Buffer IREACHItalianitalianreach
RNA Wash Buffer IIGHSEnglishenglishghs
RNA Wash Buffer IIGHSSpanishspanishghs
RNA Wash Buffer IIREACHEnglishenglishreach
RNA Wash Buffer IIREACHDanishdanishreach
RNA Wash Buffer IIREACHFinnishfinnishreach
RNA Wash Buffer IIREACHFrenchfrenchreach
RNA Wash Buffer IIREACHGermangermanreach
RNA Wash Buffer IIREACHItalianitalianreach
RNA Wash Buffer IIREACHNorwegiannorwegianreach
RNA Wash Buffer IIREACHSpanishspanishreach
RNA Wash Buffer IIREACHSwedishswedishreach
RNA Wash Buffer IIWHMSEnglishenglishwhms
RNA Wash Buffer IIWHMSFrenchfrenchwhms
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