E.Z.N.A.® Blood RNA Kit


Isolates RNA from whole blood using spin columns

The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 mL of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNase’s, the lysate is homogenized with a homogenizer spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as hemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC-treated water.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationPCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume50-100 μL
Starting MaterialWhole blood; animal tissue; others with modified protocols
Starting AmountUp to 1 mL blood; up to 30 mg tissue
RNA YieldUp to 100 µg
Processing Modemanual, centrifugation
Throughput1 – 24
RNA Binding TechnologySilica Mini spin column
Binding Capacity100 µg
HiBind® RNA Mini Columns
2 mL Collection TubesView Product
Homogenizer Mini Columns
ERL Buffer (10X)
NTL Lysis Buffer
RWF Wash Buffer
RNA Wash Buffer IIView Product
Nuclease-free WaterView Product

Figure 1. Total RNA purified using the E.Z.N.A.® Blood RNA Kit. Purified total RNA from various blood was isolated with the E.Z.N.A.® Blood RNA Kit. RNA was extracted from blood samples as follows: sample 1 from 200 µL human blood, sample 2 from 400 µL human blood, sample 3 from 1 mL human blood, sample 4 from 100 µL mouse blood, and sample 5 from 200 µL mouse blood. Total RNA (10% of total purified RNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA.

Product Manuals

Protocol Manual
1 document

Product Information

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Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
ERL BufferGHSSpanishspanishghs
ERL BufferGHSEnglishenglishghs
ERL BufferREACHGermangermanreach
ERL BufferREACHFrenchfrenchreach
ERL BufferREACHDanishdanishreach
ERL BufferREACHEnglishenglishreach
ERL BufferREACHFinnishfinnishreach
ERL BufferREACHItalianitalianreach
ERL BufferREACHNorwegiannorwegianreach
ERL BufferREACHSpanishspanishreach
ERL BufferREACHSwedishswedishreach
ERL BufferWHMSEnglishenglishwhms
ERL BufferWHMSFrenchfrenchwhms
NTL Lysis BufferGHSEnglishenglishghs
NTL Lysis BufferGHSSpanishspanishghs
NTL Lysis BufferREACHEnglishenglishreach
NTL Lysis BufferREACHDanishdanishreach
NTL Lysis BufferREACHGermangermanreach
NTL Lysis BufferREACHSpanishspanishreach
NTL Lysis BufferREACHNorwegiannorwegianreach
NTL Lysis BufferREACHItalianitalianreach
NTL Lysis BufferREACHFrenchfrenchreach
NTL Lysis BufferREACHFinnishfinnishreach
NTL Lysis BufferREACHSwedishswedishreach
NTL Lysis BufferWHMSEnglishenglishwhms
NTL Lysis BufferWHMSFrenchfrenchwhms
Nuclease-free WaterGHSEnglishenglishghs
Nuclease-free WaterGHSSpanishspanishghs
Nuclease-free WaterREACHEnglishenglishreach
Nuclease-free WaterREACHDanishdanishreach
Nuclease-free WaterREACHFinnishfinnishreach
Nuclease-free WaterREACHFrenchfrenchreach
Nuclease-free WaterREACHGermangermanreach
Nuclease-free WaterREACHSwedishswedishreach
Nuclease-free WaterREACHNorwegiannorwegianreach
Nuclease-free WaterREACHItalianitalianreach
Nuclease-free WaterREACHSpanishspanishreach
Nuclease-free WaterWHMSEnglishenglishwhms
Nuclease-free WaterWHMSFrenchfrenchwhms
Nuclease-free WaterREACHDutchdutchreach
Nuclease-free WaterREACHHungarianhungarianreach
Nuclease-free WaterREACHPortugueseportuguesereach
RNA Wash Buffer IIGHSEnglishenglishghs
RNA Wash Buffer IIGHSSpanishspanishghs
RNA Wash Buffer IIREACHEnglishenglishreach
RNA Wash Buffer IIREACHDanishdanishreach
RNA Wash Buffer IIREACHFinnishfinnishreach
RNA Wash Buffer IIREACHFrenchfrenchreach
RNA Wash Buffer IIREACHGermangermanreach
RNA Wash Buffer IIREACHSwedishswedishreach
RNA Wash Buffer IIREACHSpanishspanishreach
RNA Wash Buffer IIREACHItalianitalianreach
RNA Wash Buffer IIREACHNorwegiannorwegianreach
RNA Wash Buffer IIWHMSEnglishenglishwhms
RNA Wash Buffer IIWHMSFrenchfrenchwhms
RWF BufferGHSEnglishenglishghs
RWF BufferREACHFrenchfrenchreach
RWF BufferREACHFinnishfinnishreach
RWF BufferREACHDanishdanishreach
RWF BufferREACHEnglishenglishreach
RWF BufferGHSSpanishspanishghs
RWF BufferREACHGermangermanreach
RWF BufferREACHItalianitalianreach
RWF BufferREACHNorwegiannorwegianreach
RWF BufferREACHSpanishspanishreach
RWF BufferREACHSwedishswedishreach
RWF BufferWHMSEnglishenglishwhms
RWF BufferWHMSFrenchfrenchwhms
Nuclease-free WaterREACHGreekgreekreach
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