E.Z.N.A.® DNA/RNA Isolation Kit

$0.00$423.40

Simultaneously isolate DNA and RNA from same cells or tissues using spin column

The E.Z.N.A.® DNA/RNA Isolation Kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenized in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind® RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, the maximum yield of DNA and RNA can be purified from the entire sample.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationPCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume40-70 µL for RNA; 50-100 µL for DNA
Starting MaterialCultured cells and animal tissues
Starting AmountUp to 1X107 cells; up to 30 mg tissue
RNA YieldRNA in one fraction; DNA in another
Processing ModeManual, centrifugation
Throughput1 – 24
RNA Binding TechnologySilica Mini spin column
Binding Capacity100 µg for DNA and RNA

Figure 1. DNA/RNA was extracted from tissue samples. DNA (5% of total purified DNA) was analyzed on a 0.8% agarose gel. Total RNA (10% of total purified RNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the DNA/RNA. Sample 1 from mouse liver, sample 2 from mouse kidney, sample 3 from mouse spleen, sample 4 from HeLa cells, and sample 5 from cos-7 cells.

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Protocol Manual
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Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferWHMSFrenchfrenchwhms
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHDanishdanishreach
Elution BufferREACHEnglishenglishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
GTC Lysis BufferGHSEnglishenglishghs
GTC Lysis BufferGHSSpanishspanishghs
GTC Lysis BufferREACHSpanishspanishreach
GTC Lysis BufferREACHSwedishswedishreach
GTC Lysis BufferREACHNorwegiannorwegianreach
GTC Lysis BufferREACHItalianitalianreach
GTC Lysis BufferREACHGermangermanreach
GTC Lysis BufferREACHFrenchfrenchreach
GTC Lysis BufferREACHFinnishfinnishreach
GTC Lysis BufferREACHDanishdanishreach
GTC Lysis BufferREACHEnglishenglishreach
GTC Lysis BufferWHMSEnglishenglishwhms
GTC Lysis BufferWHMSFrenchfrenchwhms
HBC BufferGHSEnglishenglishghs
HBC BufferGHSSpanishspanishghs
HBC BufferREACHEnglishenglishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHFinnishfinnishreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHGermangermanreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHSwedishswedishreach
HBC BufferREACHSpanishspanishreach
HBC BufferWHMSEnglishenglishwhms
HBC BufferWHMSFrenchfrenchwhms
Nuclease-free WaterGHSEnglishenglishghs
Nuclease-free WaterGHSSpanishspanishghs
Nuclease-free WaterREACHEnglishenglishreach
Nuclease-free WaterREACHDanishdanishreach
Nuclease-free WaterREACHFinnishfinnishreach
Nuclease-free WaterREACHFrenchfrenchreach
Nuclease-free WaterREACHGermangermanreach
Nuclease-free WaterREACHNorwegiannorwegianreach
Nuclease-free WaterREACHSwedishswedishreach
Nuclease-free WaterREACHSpanishspanishreach
Nuclease-free WaterREACHItalianitalianreach
Nuclease-free WaterWHMSEnglishenglishwhms
Nuclease-free WaterWHMSFrenchfrenchwhms
Nuclease-free WaterREACHDutchdutchreach
Nuclease-free WaterREACHHungarianhungarianreach
Nuclease-free WaterREACHPortugueseportuguesereach
RNA Wash Buffer IGHSEnglishenglishghs
RNA Wash Buffer IREACHFinnishfinnishreach
RNA Wash Buffer IREACHDanishdanishreach
RNA Wash Buffer IGHSSpanishspanishghs
RNA Wash Buffer IREACHEnglishenglishreach
RNA Wash Buffer IREACHFrenchfrenchreach
RNA Wash Buffer IREACHGermangermanreach
RNA Wash Buffer IREACHItalianitalianreach
RNA Wash Buffer IREACHNorwegiannorwegianreach
RNA Wash Buffer IREACHSpanishspanishreach
RNA Wash Buffer IREACHSwedishswedishreach
RNA Wash Buffer IWHMSEnglishenglishwhms
RNA Wash Buffer IWHMSFrenchfrenchwhms
RNA Wash Buffer IIGHSEnglishenglishghs
RNA Wash Buffer IIGHSSpanishspanishghs
RNA Wash Buffer IIREACHEnglishenglishreach
RNA Wash Buffer IIREACHDanishdanishreach
RNA Wash Buffer IIREACHFinnishfinnishreach
RNA Wash Buffer IIREACHFrenchfrenchreach
RNA Wash Buffer IIREACHGermangermanreach
RNA Wash Buffer IIREACHItalianitalianreach
RNA Wash Buffer IIREACHNorwegiannorwegianreach
RNA Wash Buffer IIREACHSpanishspanishreach
RNA Wash Buffer IIREACHSwedishswedishreach
RNA Wash Buffer IIWHMSEnglishenglishwhms
RNA Wash Buffer IIWHMSFrenchfrenchwhms
Elution BufferREACHGreekgreekreach
Nuclease-free WaterREACHGreekgreekreach
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