E.Z.N.A.® Endo-Free Plasmid DNA Maxi Kit


  • Rapid – Purification of plasmid DNA in < 45min
  • Safe – No phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – Transfection grade, low-endotoxin plasmid DNA (< 1 EU/µg)

The E.Z.N.A.® Endo-Free Plasmid DNA Maxi Kit provides an efficient method for the large-scale isolation of low-endotoxin plasmid DNA. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis. Following lysate clearance, endotoxins are removed, and the protocol follows a simple “bind-wash-elute” procedure to deliver high-quality plasmid DNA. The yields vary according to plasmid copy number, E. coli strain, and growth conditions. 50-200 mL of bacterial cultures in LB medium typically produces 0.6-1.2 mg of high copy number plasmid DNA. Up to 500 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation. Purified plasmid DNA is suitable for sensitive downstream applications such as transfection of mammalian cells, cloning, automated fluorescent DNA sequencing (typical reads exceed 800 bp), next-generation sequencing, restriction enzyme digestion, ligation, PCR, in vitro transcription, transformation, etc.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationTransfection, gene therapy, cloning, in vitro Transcription, Next-Generation
Sequencing, PCR, Transformation, restriction digestion, ligation
Starting material50-200 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid typeHigh-copy, low-copy, cosmid DNA
Processing modeManual (centrifugation or vacuum)
Throughput1 – 24
DNA binding technologySilica Maxi Spin Column
Lysate clearance methodFiltration using Lysate clearance syringe
Processing time<45 minutes
Yield600-1200 µg for high copy-number; 50-300 µg for low copy-number
Special noteIncludes endotoxin removal step

Figure 1.  pEGFP-N1 plasmid was purified from 200 mL DH5α cultures harboring the plasmid using Omega Bio-tek’s E.Z.N.A.® Endo-Free Plasmid DNA Maxi Kit following manufacturer’s recommended protocol. Plasmid DNA was eluted in 2 mL volume and quantified using Thermo Scientific’s NanoDrop™ 2000c system. The Endotoxin concentration was estimated based on a Limulus Amoebocyte Lysate (LAL) assay.

Product Information

Document NameLanguagesLink
Quick GuideEnglish
Protocol ManualEnglish
E.Z.N.A.® Endo-Free Plasmid Midi & Maxi KitsEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferWHMSFrenchfrenchwhms
EHBC BufferGHSEnglishenglishghs
EHBC BufferGHSSpanishspanishghs
EHBC BufferREACHEnglishenglishreach
EHBC BufferREACHDanishdanishreach
EHBC BufferREACHFinnishfinnishreach
EHBC BufferREACHFrenchfrenchreach
EHBC BufferREACHGermangermanreach
EHBC BufferREACHItalianitalianreach
EHBC BufferREACHNorwegiannorwegianreach
EHBC BufferREACHSpanishspanishreach
EHBC BufferREACHSwedishswedishreach
EHBC BufferWHMSEnglishenglishwhms
EHBC BufferWHMSFrenchfrenchwhms
Endo-Free Elution BufferGHSEnglishenglishghs
Endo-Free Elution BufferGHSSpanishspanishghs
Endo-Free Elution BufferREACHEnglishenglishreach
Endo-Free Elution BufferREACHDanishdanishreach
Endo-Free Elution BufferREACHFinnishfinnishreach
Endo-Free Elution BufferREACHFrenchfrenchreach
Endo-Free Elution BufferREACHGermangermanreach
Endo-Free Elution BufferREACHItalianitalianreach
Endo-Free Elution BufferREACHNorwegiannorwegianreach
Endo-Free Elution BufferREACHSpanishspanishreach
Endo-Free Elution BufferREACHSwedishswedishreach
Endo-Free Elution BufferWHMSEnglishenglishwhms
Endo-Free Elution BufferWHMSFrenchfrenchwhms
ETR Binding BufferGHSEnglishenglishghs
ETR Binding BufferGHSSpanishspanishghs
ETR Binding BufferREACHEnglishenglishreach
ETR Binding BufferREACHDanishdanishreach
ETR Binding BufferREACHFinnishfinnishreach
ETR Binding BufferREACHFrenchfrenchreach
ETR Binding BufferREACHGermangermanreach
ETR Binding BufferREACHItalianitalianreach
ETR Binding BufferREACHNorwegiannorwegianreach
ETR Binding BufferREACHSpanishspanishreach
ETR Binding BufferREACHSwedishswedishreach
ETR Binding BufferWHMSEnglishenglishwhms
ETR Binding BufferWHMSFrenchfrenchwhms
ETR Wash BufferGHSEnglishenglishghs
ETR Wash BufferGHSSpanishspanishghs
ETR Wash BufferREACHEnglishenglishreach
ETR Wash BufferREACHDanishdanishreach
ETR Wash BufferREACHFinnishfinnishreach
ETR Wash BufferREACHFrenchfrenchreach
ETR Wash BufferREACHGermangermanreach
ETR Wash BufferREACHItalianitalianreach
ETR Wash BufferREACHNorwegiannorwegianreach
ETR Wash BufferREACHSpanishspanishreach
ETR Wash BufferREACHSwedishswedishreach
ETR Wash BufferWHMSEnglishenglishwhms
ETR Wash BufferWHMSFrenchfrenchwhms
N3 BufferGHSEnglishenglishghs
N3 BufferGHSSpanishspanishghs
N3 BufferREACHEnglishenglishreach
N3 BufferREACHDanishdanishreach
N3 BufferREACHFinnishfinnishreach
N3 BufferREACHFrenchfrenchreach
N3 BufferREACHGermangermanreach
N3 BufferREACHItalianitalianreach
N3 BufferREACHNorwegiannorwegianreach
N3 BufferREACHSpanishspanishreach
N3 BufferREACHSwedishswedishreach
N3 BufferWHMSEnglishenglishwhms
N3 BufferWHMSFrenchfrenchwhms
RNase AGHSEnglishenglishghs
RNase AGHSSpanishspanishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFinnishfinnishreach
RNase AREACHFrenchfrenchreach
RNase AREACHGermangermanreach
RNase AREACHItalianitalianreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHSpanishspanishreach
RNase AREACHSwedishswedishreach
RNase AWHMSEnglishenglishwhms
RNase AWHMSFrenchfrenchwhms
Solution IGHSEnglishenglishghs
Solution IGHSSpanishspanishghs
Solution IREACHEnglishenglishreach
Solution IREACHDanishdanishreach
Solution IREACHFinnishfinnishreach
Solution IREACHFrenchfrenchreach
Solution IREACHGermangermanreach
Solution IREACHItalianitalianreach
Solution IREACHNorwegiannorwegianreach
Solution IREACHSpanishspanishreach
Solution IREACHSwedishswedishreach
Solution IWHMSEnglishenglishwhms
Solution IWHMSFrenchfrenchwhms
Solution IIGHSEnglishenglishghs
Solution IIGHSSpanishspanishghs
Solution IIREACHEnglishenglishreach
Solution IIREACHDanishdanishreach
Solution IIREACHFinnishfinnishreach
Solution IIREACHFrenchfrenchreach
Solution IIREACHGermangermanreach
Solution IIREACHItalianitalianreach
Solution IIREACHNorwegiannorwegianreach
Solution IIREACHSpanishspanishreach
Solution IIREACHSwedishswedishreach
Solution IIWHMSEnglishenglishwhms
Solution IIWHMSFrenchfrenchwhms
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