E.Z.N.A.® Endo-free Plasmid DNA Mini Kit I

  • Rapid – 30 minutes or less
  • Safe – No phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – Endotoxins efficiently reduced (<1 EU/µg)

Product will be discontinued in 2024 and has been replaced by D6950 E.Z.N.A.® Endo-Free Plasmid DNA Mini II Kit.

Plasmid isolated with traditional purification procedures normally contains high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. Up to 5 mL of overnight cultures can be used for high copy number plasmids and 10 mL for low copy number.

The E.Z.N.A.® Endo-Free Plasmid DNA Mini Kit I integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding conditions, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Downstream ApplicationSensitive cell line transfection; gene therapy etc.
Starting Material1-5 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid TypeHigh-copy, low-copy, cosmid DNA
Processing modeManual, centrifugation
Throughput1 – 24
DNA binding technologySilica mini spin column
Lysate clearing methodCentrifugation
Processing Time<30 minutes
Yield15-25 µg for high copy-number; 0.1-5 µg for low copy-number
OtherIncludes endotoxin removal step

Figure 1.  Quality and yield of plasmid DNA purified using the E.Z.N.A.® Endo-Free Plasmid DNA Mini Kit. Plasmid DNA was isolated from 3 mL of 3 different bacterial cultures. DNA was eluted in 100 µL endotoxin-free elution buffer and quantitated using Thermo Fisher’s NanoDrop® 2000 and dynamic turbidity method.

Product Information

Document NameLanguagesLink
Protocol ManualEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferWHMSFrenchfrenchwhms
Endo-Free Elution BufferGHSEnglishenglishghs
Endo-Free Elution BufferGHSSpanishspanishghs
Endo-Free Elution BufferREACHEnglishenglishreach
Endo-Free Elution BufferREACHDanishdanishreach
Endo-Free Elution BufferREACHFinnishfinnishreach
Endo-Free Elution BufferREACHFrenchfrenchreach
Endo-Free Elution BufferREACHGermangermanreach
Endo-Free Elution BufferREACHItalianitalianreach
Endo-Free Elution BufferREACHNorwegiannorwegianreach
Endo-Free Elution BufferREACHSpanishspanishreach
Endo-Free Elution BufferREACHSwedishswedishreach
Endo-Free Elution BufferWHMSEnglishenglishwhms
Endo-Free Elution BufferWHMSFrenchfrenchwhms
ETR Binding BufferGHSEnglishenglishghs
ETR Binding BufferGHSSpanishspanishghs
ETR Binding BufferREACHEnglishenglishreach
ETR Binding BufferREACHDanishdanishreach
ETR Binding BufferREACHFinnishfinnishreach
ETR Binding BufferREACHFrenchfrenchreach
ETR Binding BufferREACHGermangermanreach
ETR Binding BufferREACHItalianitalianreach
ETR Binding BufferREACHNorwegiannorwegianreach
ETR Binding BufferREACHSpanishspanishreach
ETR Binding BufferREACHSwedishswedishreach
ETR Binding BufferWHMSEnglishenglishwhms
ETR Binding BufferWHMSFrenchfrenchwhms
ETR Wash BufferGHSEnglishenglishghs
ETR Wash BufferGHSSpanishspanishghs
ETR Wash BufferREACHEnglishenglishreach
ETR Wash BufferREACHDanishdanishreach
ETR Wash BufferREACHFinnishfinnishreach
ETR Wash BufferREACHFrenchfrenchreach
ETR Wash BufferREACHGermangermanreach
ETR Wash BufferREACHItalianitalianreach
ETR Wash BufferREACHNorwegiannorwegianreach
ETR Wash BufferREACHSpanishspanishreach
ETR Wash BufferREACHSwedishswedishreach
ETR Wash BufferWHMSEnglishenglishwhms
ETR Wash BufferWHMSFrenchfrenchwhms
HBC BufferGHSEnglishenglishghs
HBC BufferGHSSpanishspanishghs
HBC BufferREACHEnglishenglishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHFinnishfinnishreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHGermangermanreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHSpanishspanishreach
HBC BufferREACHSwedishswedishreach
HBC BufferWHMSEnglishenglishwhms
HBC BufferWHMSFrenchfrenchwhms
N3 BufferGHSEnglishenglishghs
N3 BufferGHSSpanishspanishghs
N3 BufferREACHEnglishenglishreach
N3 BufferREACHDanishdanishreach
N3 BufferREACHFinnishfinnishreach
N3 BufferREACHFrenchfrenchreach
N3 BufferREACHGermangermanreach
N3 BufferREACHItalianitalianreach
N3 BufferREACHNorwegiannorwegianreach
N3 BufferREACHSpanishspanishreach
N3 BufferREACHSwedishswedishreach
N3 BufferWHMSEnglishenglishwhms
N3 BufferWHMSFrenchfrenchwhms
RNase AGHSEnglishenglishghs
RNase AGHSSpanishspanishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFinnishfinnishreach
RNase AREACHFrenchfrenchreach
RNase AREACHGermangermanreach
RNase AREACHItalianitalianreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHSpanishspanishreach
RNase AREACHSwedishswedishreach
RNase AWHMSEnglishenglishwhms
RNase AWHMSFrenchfrenchwhms
Solution IGHSEnglishenglishghs
Solution IGHSSpanishspanishghs
Solution IREACHEnglishenglishreach
Solution IREACHDanishdanishreach
Solution IREACHFinnishfinnishreach
Solution IREACHFrenchfrenchreach
Solution IREACHGermangermanreach
Solution IREACHItalianitalianreach
Solution IREACHNorwegiannorwegianreach
Solution IREACHSpanishspanishreach
Solution IREACHSwedishswedishreach
Solution IWHMSEnglishenglishwhms
Solution IWHMSFrenchfrenchwhms
Solution IIGHSEnglishenglishghs
Solution IIGHSSpanishspanishghs
Solution IIREACHEnglishenglishreach
Solution IIREACHDanishdanishreach
Solution IIREACHFinnishfinnishreach
Solution IIREACHFrenchfrenchreach
Solution IIREACHGermangermanreach
Solution IIREACHItalianitalianreach
Solution IIREACHNorwegiannorwegianreach
Solution IIREACHSpanishspanishreach
Solution IIREACHSwedishswedishreach
Solution IIWHMSEnglishenglishwhms
Solution IIWHMSFrenchfrenchwhms
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