E.Z.N.A.® FastFilter Plasmid DNA Mini Kit


  • Rapid – 9 Minute Processing time from a bacterial pellet
  • Unique – Lysate Clearance and DNA Binding in 1 step
  • Convenient – Lysate Clearance Column eliminates cellular debris pelleting and transfer step
  • Versatile – Process up to 5 mL bacterial cultures with different plasmid types and culture medias

The E.Z.N.A.® FastFilter Plasmid DNA Mini Kit is designed for rapid purification of high-quality plasmid DNA from up to 5 mL of culture following the alkaline-lysis method. The kit features an innovative and first-of-its-kind FastFilter Mini Column nested within a regular HiBind® DNA Mini Column to combine lysate clearance and DNA binding into one or two centrifugation steps depending on the culture volume. The protocol time is just 9 minutes when starting with bacterial pellet from up to 3 mL culture volume. The protocol time for culture volumes greater than 3 mL will be slightly longer due to two centrifugation steps for lysate clearance. Following the wash and elute steps, purified plasmid DNA is immediately ready for various downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR, and DNA sequencing.

Typically, up to 5 mL overnight bacterial culture in LB or other suitable growth medium is centrifuged to pellet the bacterial cells. The cells are then lysed under alkaline conditions and subsequently neutralized to form white flocculent precipitate. Neutralized lysate is then transferred to a FastFilter Mini Column nested within a HiBind® DNA Mini Column. Upon adjustment of binding conditions, the lysate is centrifuged through this FastFilter HiBind® configuration to both clear the lysate and facilitate binding of DNA to the HiBind®
DNA Mini Column. The FastFilter Mini Column is discarded, and the HiBind® DNA Mini Column is subjected to two wash steps to remove salt and other contaminants. High-quality plasmid DNA is then eluted in low-salt elution buffer and is ready for use in a wide range of downstream applications mentioned above.

For Research Use Only. Not for use in diagnostic procedures.

Starting MaterialBacteria harboring high-copy or low-copy plasmid in LB culture medium
Starting Amount1-5 mL LB culture
Elution Volume50-100 µL
Processing modeManual (centrifugation or vacuum)
DNA binding technologySilica Mini Spin Column
Processing time9 minutes
Downstream ApplicationsRoutine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing
ItemAvailable Separately
HiBind® DNA Mini ColumnsView Product
2 mL Collection TubesView Product
FastFilter Mini Column---
Solution IView Product
Solution IIView Product
N3 Buffer---
GBT Buffer---
HBC BufferView Product
DNA Wash BufferView Product
RNase AView Product
Elution BufferView Product

High Yield Across Various Plasmid Types and Culture Media

Figure 1. E.Z.N.A.® FastFilter Plasmid DNA Mini Kit is capable of handling diverse sample input conditions ranging from plasmid types, culture medium, and input culture volume. The plasmid yield my vary based on these conditions. 

Comparison of Processing Time

Figure 2. Comparison of Plasmid Purification times (n=4) using kits from Omega Bio-tek, Company Z, or Company Q. The processing time was comparable using kits from Omega Bio-tek and Company Z at minimum culture volume and was lower by 2 min for Omega Bio-tek at maximum volume. The processing times shown are actual timed extractions including all pipetting, centrifugation, and bacterial culture pelleting steps. 

Competior Analysis of Plasmid DNA Purification

Table 1. pGEM plsmid was purified DH5α cultures (n=4) using kits from Omega Bio-tek, Company Z, or Company Q following manufacturer’s recommended centrifugation protocol. Purified Plasmid DNA was eluted at 100 µL irrespective of the manufacturing kit used and quantified using Thermo Scientific’s NanoDrop™ 200c system. High Yield and Quality are not sacrificed for faster processing times. 

High-Quality Plasmid DNA Suitable for Sequencing Reactions

Table 2. Plasmid DNA purified using the E.Z.N.A.® FastFilter Plasmid DNA Mini Kit was used in a 5 µL Sanger sequencing reaction. The sequencing reaction was analyzed on an Applied Biosystems 3730XL. The samples had an average CRL of 994 bp and an average QV20+ of 985 with a quality score greater than 20 (≤ 1% probability of error in base calling).

Complete Digestion with Various Restriction Enzymes

Figure 3. pGEM Plasmid DNA from overnight bacterial cultures was purified using the E.Z.N.A.® FastFilter Plasmid DNA Mini Kit and analyzed by restriction digestion. Complete digestion was observed for the various restriction enzymes tested. 

High Yield and Quality

Figure 4. pGEM Plasmid DNA from overnight bacterial cultures was purified using the E.Z.N.A.® FastFilter Plasmid DNA Mini Kit and analyzed by gel electrophoresis to illustrate the resulting well-defined band. 

Product Information

Document NameLanguagesLink
Protocol ManualEnglish
E.Z.N.A.® FastFilter Plasmid DNA Mini KitEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferWHMSFrenchfrenchwhms
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
GBT BufferGHSEnglishenglishghs
GBT BufferGHSSpanishspanishghs
GBT BufferREACHEnglishenglishreach
GBT BufferREACHDanishdanishreach
GBT BufferREACHFinnishfinnishreach
GBT BufferREACHFrenchfrenchreach
GBT BufferREACHGermangermanreach
GBT BufferREACHItalianitalianreach
GBT BufferREACHNorwegiannorwegianreach
GBT BufferREACHSpanishspanishreach
GBT BufferREACHSwedishswedishreach
GBT BufferWHMSEnglishenglishwhms
GBT BufferWHMSFrenchfrenchwhms
HBC BufferGHSEnglishenglishghs
HBC BufferGHSSpanishspanishghs
HBC BufferREACHEnglishenglishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHFinnishfinnishreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHGermangermanreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHSpanishspanishreach
HBC BufferREACHSwedishswedishreach
HBC BufferWHMSEnglishenglishwhms
HBC BufferWHMSFrenchfrenchwhms
N3 BufferGHSEnglishenglishghs
N3 BufferGHSSpanishspanishghs
N3 BufferREACHEnglishenglishreach
N3 BufferREACHDanishdanishreach
N3 BufferREACHFinnishfinnishreach
N3 BufferREACHFrenchfrenchreach
N3 BufferREACHGermangermanreach
N3 BufferREACHItalianitalianreach
N3 BufferREACHNorwegiannorwegianreach
N3 BufferREACHSpanishspanishreach
N3 BufferREACHSwedishswedishreach
N3 BufferWHMSEnglishenglishwhms
N3 BufferWHMSFrenchfrenchwhms
RNase AGHSEnglishenglishghs
RNase AGHSSpanishspanishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFinnishfinnishreach
RNase AREACHFrenchfrenchreach
RNase AREACHGermangermanreach
RNase AREACHItalianitalianreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHSpanishspanishreach
RNase AREACHSwedishswedishreach
RNase AWHMSEnglishenglishwhms
RNase AWHMSFrenchfrenchwhms
Solution IGHSEnglishenglishghs
Solution IGHSSpanishspanishghs
Solution IREACHEnglishenglishreach
Solution IREACHDanishdanishreach
Solution IREACHFinnishfinnishreach
Solution IREACHFrenchfrenchreach
Solution IREACHGermangermanreach
Solution IREACHItalianitalianreach
Solution IREACHNorwegiannorwegianreach
Solution IREACHSpanishspanishreach
Solution IREACHSwedishswedishreach
Solution IWHMSEnglishenglishwhms
Solution IWHMSFrenchfrenchwhms
Solution IIGHSEnglishenglishghs
Solution IIGHSSpanishspanishghs
Solution IIREACHEnglishenglishreach
Solution IIREACHDanishdanishreach
Solution IIREACHFinnishfinnishreach
Solution IIREACHFrenchfrenchreach
Solution IIREACHGermangermanreach
Solution IIREACHItalianitalianreach
Solution IIREACHNorwegiannorwegianreach
Solution IIREACHSpanishspanishreach
Solution IIREACHSwedishswedishreach
Solution IIWHMSEnglishenglishwhms
Solution IIWHMSFrenchfrenchwhms
Elution BufferREACHGreekgreekreach
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