E.Z.N.A.® FastFilter Plasmid DNA Mini Kit


  • Rapid – 9 Minute Processing time from a bacterial pellet
  • Unique – Lysate Clearance and DNA Binding in 1 step
  • Convenient – Lysate Clearance Column eliminates cellular debris pelleting and transfer step
  • Versatile – Process up to 5 mL bacterial cultures with different plasmid types and culture medias

The E.Z.N.A.® Plasmid DNA Mini Kit I is designed to isolate up to 25 µg of high-quality plasmid DNA from 1-5 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with HiBind® Mini Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.

The E.Z.N.A.® Plasmid DNA Mini Kit is available in two formats – Q-spin (D6942) and V-spin (D6943). D6942 (Q-spin) features columns that are capless whereas D6943 (V-spin) includes columns that have a cap attached. The columns are otherwise identical in use and application and can be used in both vacuum or centrifugation protocols.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationCloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material1-5 mL LB culture
Plasmid typeHigh-copy, low-copy, cosmid DNA
Processing modeManual (centrifugation or vacuum)
DNA binding technologySilica Mini Spin Column
Lysate clearance methodCentrifugation
Processing time<30 minutes
Yield15-25 µg for high copy-number; 0.1-5 µg for low copy-number
Special noteThe alternative for discontinued yeast plasmid kit
HiBind® DNA Mini ColumnsView Product
2 mL Collection TubesView Product
Solution IView Product
Solution IIView Product
Solution IIIView Product
HBC BufferView Product
DNA Wash BufferView Product
RNase AView Product
Elution BufferView Product

High Yield Across Various Plasmid Types and Culture Media

Figure 1. E.Z.N.A.® FastFilter Plasmid DNA Mini Kit is capable of handling diverse sample input conditions ranging from plasmid types, culture medium, and input culture volume. The plasmid yield my vary based on these conditions. 

Comparison of Processing Time

Figure 2. Comparison of Plasmid Purification times (n=4) using kits from Omega Bio-tek, Company Z, or Company Q. The processing time was comparable using kits from Omega Bio-tek and Company Z at minimum culture volume and was lower by 2 min for Omega Bio-tek at maximum volume. The processing times shown are actual timed extractions including all pipetting, centrifugation, and bacterial culture pelleting steps. 

Competior Analysis of Plasmid DNA Purification

Table 1. pGEM plsmid was purified DH5α cultures (n=4) using kits from Omega Bio-tek, Company Z, or Company Q following manufacturer’s recommended centrifugation protocol. Purified Plasmid DNA was eluted at 100 µL irrespective of the manufacturing kit used and quantified using Thermo Scientific’s NanoDrop™ 200c system. High Yield and Quality are not sacrificed for faster processing times. 

High-Quality Plasmid DNA Suitable for Sequencing Reactions

Table 2. Plasmid DNA purified using the E.Z.N.A.® FastFilter Plasmid DNA Mini Kit was used in a 5 µL Sanger sequencing reaction. The sequencing reaction was analyzed on an Applied Biosystems 3730XL. The samples had an average CRL of 994 bp and an average QV20+ of 985 with a quality score greater than 20 (≤ 1% probability of error in base calling).

Complete Digestion with Various Restriction Enzymes

Figure 3. pGEM Plasmid DNA from overnight bacterial cultures was purified using the E.Z.N.A.® FastFilter Plasmid DNA Mini Kit and analyzed by restriction digestion. Complete digestion was observed for the various restriction enzymes tested. 

High Yield and Quality

Figure 4. pGEM Plasmid DNA from overnight bacterial cultures was purified using the E.Z.N.A.® FastFilter Plasmid DNA Mini Kit and analyzed by gel electrophoresis to illustrate the resulting well-defined band. 

Product Information

Document NameLanguagesLink
E.Z.N.A.® FastFilter Plasmid DNA Mini KitEnglish
Protocol ManualEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferWHMSFrenchfrenchwhms
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHGreekgreekreach
GBT BufferGHSEnglishenglishghs
GBT BufferGHSSpanishspanishghs
GBT BufferREACHEnglishenglishreach
GBT BufferREACHDanishdanishreach
GBT BufferREACHFinnishfinnishreach
GBT BufferREACHFrenchfrenchreach
GBT BufferREACHGermangermanreach
GBT BufferREACHItalianitalianreach
GBT BufferREACHNorwegiannorwegianreach
GBT BufferREACHSpanishspanishreach
GBT BufferREACHSwedishswedishreach
GBT BufferWHMSEnglishenglishwhms
GBT BufferWHMSFrenchfrenchwhms
HBC BufferGHSEnglishenglishghs
HBC BufferGHSSpanishspanishghs
HBC BufferREACHEnglishenglishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHFinnishfinnishreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHGermangermanreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHSpanishspanishreach
HBC BufferREACHSwedishswedishreach
HBC BufferWHMSEnglishenglishwhms
HBC BufferWHMSFrenchfrenchwhms
N3 BufferGHSEnglishenglishghs
N3 BufferGHSSpanishspanishghs
N3 BufferREACHEnglishenglishreach
N3 BufferREACHDanishdanishreach
N3 BufferREACHFinnishfinnishreach
N3 BufferREACHFrenchfrenchreach
N3 BufferREACHGermangermanreach
N3 BufferREACHItalianitalianreach
N3 BufferREACHNorwegiannorwegianreach
N3 BufferREACHSpanishspanishreach
N3 BufferREACHSwedishswedishreach
N3 BufferWHMSEnglishenglishwhms
N3 BufferWHMSFrenchfrenchwhms
RNase AGHSEnglishenglishghs
RNase AGHSSpanishspanishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFinnishfinnishreach
RNase AREACHFrenchfrenchreach
RNase AREACHGermangermanreach
RNase AREACHItalianitalianreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHSpanishspanishreach
RNase AREACHSwedishswedishreach
RNase AWHMSEnglishenglishwhms
RNase AWHMSFrenchfrenchwhms
Solution IGHSEnglishenglishghs
Solution IGHSSpanishspanishghs
Solution IREACHEnglishenglishreach
Solution IREACHDanishdanishreach
Solution IREACHFinnishfinnishreach
Solution IREACHFrenchfrenchreach
Solution IREACHGermangermanreach
Solution IREACHItalianitalianreach
Solution IREACHNorwegiannorwegianreach
Solution IREACHSpanishspanishreach
Solution IREACHSwedishswedishreach
Solution IWHMSEnglishenglishwhms
Solution IWHMSFrenchfrenchwhms
Solution IIGHSEnglishenglishghs
Solution IIGHSSpanishspanishghs
Solution IIREACHEnglishenglishreach
Solution IIREACHDanishdanishreach
Solution IIREACHFinnishfinnishreach
Solution IIREACHFrenchfrenchreach
Solution IIREACHGermangermanreach
Solution IIREACHItalianitalianreach
Solution IIREACHNorwegiannorwegianreach
Solution IIREACHSpanishspanishreach
Solution IIREACHSwedishswedishreach
Solution IIWHMSEnglishenglishwhms
Solution IIWHMSFrenchfrenchwhms
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