Isolates RNA from the FFPE samples using spin columns

The E.Z.N.A.® FFPE RNA Kit is specially designed for isolation of total RNA from formalin-fixed, paraffin-embedded tissue sections. This method can also be used for the preparation of total RNA from fibrous samples such as heart and muscle tissue. The sample is first processed in a special buffer by heating to deactivate RNase’s and deparaffinization. Then the lysate is treated with Proteinase K to digest proteins. The cleared lysate is then applied to a HiBind RNA column to capture RNA. After two quick wash steps, purified RNA is eluted with water. While E.Z.N.A.® FFPE RNA Kit is optimized to minimize the effect of formaldehyde modification, it is not recommended to use the RNA purified from this kit for downstream applications that require full-length RNA.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationPCR, qPCR, real-time RT-PCR, micro-array, Northern blot, poly-A purification
Elution Volume15-30 μL
Starting MaterialFFPE tissue
Starting Amount3-4 sections with a thickness of 10 μm
RNA YieldUp to 50 µg
Processing ModeManual, centrifugation
Throughput1 – 24
RNA Binding TechnologySilica Micro spin column
Binding Capacity50 µg
ItemAvailable Separately
MicroElute® LE RNA ColumnsView Product
DNA Clearance Columns
2 mL Collection TubesView Product
FTL2 Buffer
GFC Buffer
RNA Wash Buffer IIView Product
Proteinase K SolutionView Product
Nuclease-free Water

Figure 1. FFPE samples from chicken liver and fish liver purified with E.Z.N.A. FFPE RNA Kit.

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Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
GFC BufferGHSSpanishspanishghs
GFC BufferGHSEnglishenglishghs
GFC BufferREACHEnglishenglishreach
GFC BufferREACHDanishdanishreach
GFC BufferREACHFinnishfinnishreach
GFC BufferREACHFrenchfrenchreach
GFC BufferREACHGermangermanreach
GFC BufferREACHItalianitalianreach
GFC BufferREACHNorwegiannorwegianreach
GFC BufferREACHSpanishspanishreach
GFC BufferREACHSwedishswedishreach
GFC BufferWHMSEnglishenglishwhms
GFC BufferWHMSFrenchfrenchwhms
GPL BufferGHSEnglishenglishghs
GPL BufferGHSSpanishspanishghs
GPL BufferREACHEnglishenglishreach
GPL BufferREACHItalianitalianreach
GPL BufferREACHGermangermanreach
GPL BufferREACHFinnishfinnishreach
GPL BufferREACHDanishdanishreach
GPL BufferREACHFrenchfrenchreach
GPL BufferREACHNorwegiannorwegianreach
GPL BufferREACHSpanishspanishreach
GPL BufferREACHSwedishswedishreach
GPL BufferWHMSEnglishenglishwhms
GPL BufferWHMSFrenchfrenchwhms
RNA Wash Buffer IIGHSEnglishenglishghs
RNA Wash Buffer IIGHSSpanishspanishghs
RNA Wash Buffer IIREACHEnglishenglishreach
RNA Wash Buffer IIREACHDanishdanishreach
RNA Wash Buffer IIREACHFinnishfinnishreach
RNA Wash Buffer IIREACHFrenchfrenchreach
RNA Wash Buffer IIREACHGermangermanreach
RNA Wash Buffer IIREACHItalianitalianreach
RNA Wash Buffer IIREACHNorwegiannorwegianreach
RNA Wash Buffer IIREACHSpanishspanishreach
RNA Wash Buffer IIREACHSwedishswedishreach
RNA Wash Buffer IIWHMSEnglishenglishwhms
RNA Wash Buffer IIWHMSFrenchfrenchwhms
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