E.Z.N.A.® Fungal RNA Mini Kit


Isolate total RNA from fungal samples using spin columns

Modified from the E.Z.N.A.® Plant RNA Kit, the E.Z.N.A.® Fungal RNA Kit uses an improved buffer system that is more efficient for fungal samples. This kit provides convenient and rapid methods for the isolation of total RNA from a variety of fungal species. Rather, the method involves a simple and rapid precipitation step for removal of much of the polysaccharides and phenolic compounds commonly found in fungal tissues. In combination with HiBind ® RNA spin columns, this method permits purification of high-quality RNA from as much as 200 mg tissue. The system is also efficient enough to purify total RNA from as little as 10 mg of tissue or 100 cells. Purified RNA can be used for many downstream applications such as RT-PCR, Northern Blotting, Poly A +(mRNA) purification, and differential display.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationPCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume50-100 μL
Starting MaterialFungal Tissue
Starting Amount<250 mg
RNA YieldUp to 100 µg, >200 nt
Processing ModeSpin column
Throughput1 – 24
RNA Binding Technology1 – 24
Binding Capacity100 µg
HiBind® RNA Mini Columns
Homogenizer Mini Columns
2 mL Collection TubesView Product
NTL Lysis Buffer
SP Buffer
RB Buffer
RNA Wash Buffer IView Product
RNA Wash Buffer IIView Product
Nuclease-free Water

Figure 1. Total RNA purified using the E.Z.N.A.® Fungal RNA Kit. Purified total RNA from 100 mg fungal species was isolated with the E.Z.N.A.® Fungal RNA Kit. RNA was extracted from fungal species as follows: sample 1 from E. edodes, sample 2 from V. volvacea, sample 3 from F. velutipes, sample 4 from H. erinaceum. Total RNA (10% of total purified RNA) was analyzed to demonstrate yield and quality of the RNA.

Product Manuals

Protocol Manual
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Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
NTL Lysis BufferGHSSpanishspanishghs
NTL Lysis BufferREACHEnglishenglishreach
NTL Lysis BufferREACHDanishdanishreach
NTL Lysis BufferGHSEnglishenglishghs
NTL Lysis BufferREACHNorwegiannorwegianreach
NTL Lysis BufferREACHSpanishspanishreach
NTL Lysis BufferREACHItalianitalianreach
NTL Lysis BufferREACHGermangermanreach
NTL Lysis BufferREACHFrenchfrenchreach
NTL Lysis BufferREACHFinnishfinnishreach
NTL Lysis BufferREACHSwedishswedishreach
NTL Lysis BufferWHMSEnglishenglishwhms
NTL Lysis BufferWHMSFrenchfrenchwhms
Nuclease-free WaterGHSEnglishenglishghs
Nuclease-free WaterREACHGermangermanreach
Nuclease-free WaterREACHFrenchfrenchreach
Nuclease-free WaterREACHFinnishfinnishreach
Nuclease-free WaterREACHEnglishenglishreach
Nuclease-free WaterGHSSpanishspanishghs
Nuclease-free WaterREACHDanishdanishreach
Nuclease-free WaterREACHItalianitalianreach
Nuclease-free WaterREACHNorwegiannorwegianreach
Nuclease-free WaterREACHSpanishspanishreach
Nuclease-free WaterREACHSwedishswedishreach
Nuclease-free WaterREACHHungarianhungarianreach
Nuclease-free WaterREACHPortugueseportuguesereach
Nuclease-free WaterREACHDutchdutchreach
Nuclease-free WaterWHMSFrenchfrenchwhms
Nuclease-free WaterWHMSEnglishenglishwhms
RB BufferGHSEnglishenglishghs
RB BufferGHSSpanishspanishghs
RB BufferREACHEnglishenglishreach
RB BufferREACHDanishdanishreach
RB BufferREACHFinnishfinnishreach
RB BufferREACHFrenchfrenchreach
RB BufferWHMSFrenchfrenchwhms
RB BufferWHMSEnglishenglishwhms
RB BufferREACHSwedishswedishreach
RB BufferREACHNorwegiannorwegianreach
RB BufferREACHSpanishspanishreach
RB BufferREACHGermangermanreach
RB BufferREACHItalianitalianreach
RNA Wash Buffer IGHSEnglishenglishghs
RNA Wash Buffer IGHSSpanishspanishghs
RNA Wash Buffer IREACHEnglishenglishreach
RNA Wash Buffer IREACHDanishdanishreach
RNA Wash Buffer IREACHFinnishfinnishreach
RNA Wash Buffer IREACHFrenchfrenchreach
RNA Wash Buffer IREACHGermangermanreach
RNA Wash Buffer IREACHItalianitalianreach
RNA Wash Buffer IREACHNorwegiannorwegianreach
RNA Wash Buffer IREACHSpanishspanishreach
RNA Wash Buffer IIGHSEnglishenglishghs
RNA Wash Buffer IWHMSFrenchfrenchwhms
RNA Wash Buffer IWHMSEnglishenglishwhms
RNA Wash Buffer IREACHSwedishswedishreach
RNA Wash Buffer IIGHSSpanishspanishghs
RNA Wash Buffer IIREACHEnglishenglishreach
RNA Wash Buffer IIREACHDanishdanishreach
RNA Wash Buffer IIREACHGermangermanreach
RNA Wash Buffer IIREACHFrenchfrenchreach
RNA Wash Buffer IIREACHFinnishfinnishreach
RNA Wash Buffer IIREACHItalianitalianreach
RNA Wash Buffer IIREACHNorwegiannorwegianreach
RNA Wash Buffer IIREACHSpanishspanishreach
RNA Wash Buffer IIREACHSwedishswedishreach
RNA Wash Buffer IIWHMSEnglishenglishwhms
RNA Wash Buffer IIWHMSFrenchfrenchwhms
SP BufferREACHDanishdanishreach
SP BufferREACHFrenchfrenchreach
SP BufferREACHFinnishfinnishreach
SP BufferREACHEnglishenglishreach
SP BufferGHSSpanishspanishghs
SP BufferGHSEnglishenglishghs
SP BufferREACHGermangermanreach
SP BufferREACHItalianitalianreach
SP BufferREACHNorwegiannorwegianreach
SP BufferREACHSpanishspanishreach
SP BufferREACHSwedishswedishreach
SP BufferWHMSEnglishenglishwhms
SP BufferWHMSFrenchfrenchwhms
Nuclease-free WaterREACHGreekgreekreach
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