E.Z.N.A.® HP Plant & Fungal DNA Kit


Isolate DNA from plant or fungal sample with organic solvents using spin columns.

  • Reliable – Reproducible DNA purification from variety of sample sources
  • High-quality – Purified DNA suitable for any application
  • High yield – Efficient purification of DNA from even specialized samples

The E.Z.N.A.® High Performance (HP) Plant & Fungal DNA Kit is designed for efficient
recovery of genomic DNA from fresh, frozen, and dried plant or fungal tissue samples
rich in polysaccharides or having lower DNA content. Up to 100 mg wet tissue (or 50 mg
dry tissue) can be processed in less than 1 hour. The system combines the reversible
nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of
spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme
inhibitors from plant or fungal tissue lysates. Purified DNA is suitable for PCR, restriction
digestion, and hybridization techniques.

If using the E.Z.N.A.® HP Plant & Fungal DNA Kit for the first time, please read this booklet
to become familiar with the procedures. This procedure relies on the well established
properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in
conjunction with the selective DNA binding of Omega Bio-tek’s HiBind® matrix, to purify
high-quality DNA. Samples are homogenized and lysed in a high salt buffer containing
CTAB and extracted with chloroform to remove polysaccharides and other components
that interfere with many routine DNA isolations and downstream applications. Binding
conditions are adjusted and DNA is further purified using HiBind ® DNA Mini Columns.
In this way, salts, proteins, and other contaminants are removed to yield high-quality
genomic DNA suitable for downstream applications such as endonuclease digestion,
thermal cycle amplification, and hybridization.

For Research Use Only. Not for use in diagnostic procedures.

Starting Amount100 mg of wet sample or 30 mg dry sample
Starting MaterialFresh and dried plant tissue samples rich in lipids, polyphenols, polysaccharides, or those with lower DNA content.
Elution Volume50-100 μL
TechnologyHiBind® DNA Mini Column
Processing ModeManual

Figure 1. Purified total cellular DNA from various plant sources was isolated with the DNeasy Plant Kit and the E.Z.N.A.® HP Plant & Fungal DNA Kit. DNA was extracted from 100 mg fresh leaves from the following plants: lanes 2-5 from maize, lanes 6-9 from peanut, lanes 10-13 from tomato, and lanes 14-17 from soybean. Total cellular DNA (10% of total purified DNA) was analyzed on a 0.8% agarose gel to demonstrate yield and quality of the DNA. M: Lambda Hind III DNA Marker.

Product Information

Protocol ManualProduct ManualEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
CSPL BufferGHSSpanishspanishghs
CSPL BufferGHSEnglishenglishghs
CSPL BufferWHMSFrenchfrenchwhms
CSPL BufferWHMSEnglishenglishwhms
CSPL BufferREACHSwedishswedishreach
CSPL BufferREACHSpanishspanishreach
CSPL BufferREACHNorwegiannorwegianreach
CSPL BufferREACHItalianitalianreach
CSPL BufferREACHGermangermanreach
CSPL BufferREACHFrenchfrenchreach
CSPL BufferREACHFinnishfinnishreach
CSPL BufferREACHDanishdanishreach
CSPL BufferREACHEnglishenglishreach
CXD BufferREACHDanishdanishreach
CXD BufferWHMSFrenchfrenchwhms
CXD BufferWHMSEnglishenglishwhms
CXD BufferREACHSwedishswedishreach
CXD BufferREACHSpanishspanishreach
CXD BufferREACHNorwegiannorwegianreach
CXD BufferREACHItalianitalianreach
CXD BufferREACHGermangermanreach
CXD BufferREACHFrenchfrenchreach
CXD BufferREACHFinnishfinnishreach
CXD BufferREACHEnglishenglishreach
CXD BufferGHSSpanishspanishghs
CXD BufferGHSEnglishenglishghs
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferWHMSFrenchfrenchwhms
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferREACHGermangermanreach
Elution BufferREACHGreekgreekreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHDutchdutchreach
Elution BufferWHMSFrenchfrenchwhms
Elution BufferWHMSEnglishenglishwhms
Elution BufferREACHSwedishswedishreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHGermangermanreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHEnglishenglishreach
Elution BufferGHSSpanishspanishghs
Elution BufferGHSEnglishenglishghs
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