E.Z.N.A.® M13 DNA Mini Kit
Price range: $0.00 through $113.50
Isolate single-stranded DNA from up to 3 mL of phage supernatant using spin mini columns
The E.Z.N.A.® family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is Omega Bio-tek’s HiBind® matrix that specifically, but reversibly, binds DNA or RNA under optimal conditions while allowing proteins and other contaminants to be removed. The nucleic acids bound to the HiBind® matrix are easily eluted with deionized water or low salt buffer. DNA and RNA are suitable for many downstream applications.
Omega Bio-tek’s E.Z.N.A.® M13 DNA Kits are designed to purify up to 10 µg of single stranded DNA from up to 3 mL of phage supernatant. Yields of single stranded DNA obtained using E.Z.N.A.® M13 DNA Kits are around 3-10 µg and reproducible when the isolations are performed from the same culture.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Application | Single-stranded M13 DNA |
| Starting material | 3 mL phage supernatant |
| Plasmid type | Single-stranded M13 DNA |
| Processing mode | Manual, centrifugation |
| Throughput | 1 – 24 |
| DNA binding technology | Silica Mini Spin Column |
| Lysate clearance method | Not needed |
| Yield | 3-10 µg single-stranded DNA |
Figure 1. DNA purified using the E.Z.N.A.® M13 DNA Kit. DNA was isolated from 4 1 mL M13 supernatant cultures grown 6 hours in E. coli culture using the E.Z.N.A.® M13 DNA Kit and eluted in 50 µL TE buffer. M13 ssDNA (5% of total purified DNA) was analyzed on a 1.0% agarose gel. Lanes 1-4: M13 ssDNA, M: 1 kb DNA formaldehyde agarose gel..