E.Z.N.A.® M13 DNA Mini Kit


Isolate single-stranded DNA from up to 3 mL of phage supernatant using spin mini columns

The E.Z.N.A.® family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is Omega Bio-tek’s HiBind® matrix that specifically, but reversibly, binds DNA or RNA under optimal conditions while allowing proteins and other contaminants to be removed. The nucleic acids bound to the HiBind® matrix are easily eluted with deionized water or low salt buffer. DNA and RNA are suitable for many downstream applications.

Omega Bio-tek’s E.Z.N.A.® M13 DNA Kits are designed to purify up to 10 µg of single stranded DNA from up to 3 mL of phage supernatant. Yields of single stranded DNA obtained using E.Z.N.A.® M13 DNA Kits are around 3-10 µg and reproducible when the isolations are performed from the same culture.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationSingle-stranded M13 DNA
Starting material3 mL phage supernatant
Plasmid typeSingle-stranded M13 DNA
Processing modeManual, centrifugation
Throughput1 – 24
DNA binding technologySilica Mini Spin Column
Lysate clearance methodNot needed
Yield 3-10 µg single-stranded DNA

Figure 1.  DNA purified using the E.Z.N.A.® M13 DNA Kit. DNA was isolated from 4 1 mL M13 supernatant cultures grown 6 hours in E. coli culture using the E.Z.N.A.® M13 DNA Kit and eluted in 50 µL TE buffer. M13 ssDNA (5% of total purified DNA) was analyzed on a 1.0% agarose gel. Lanes 1-4: M13 ssDNA, M: 1 kb DNA formaldehyde agarose gel..

Product Information

Document NameLanguagesLink
Protocol ManualEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
Elution BufferGHSSpanishspanishghs
Elution BufferGHSEnglishenglishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
MPG BufferGHSEnglishenglishghs
MPG BufferREACHEnglishenglishreach
MPG BufferGHSSpanishspanishghs
MPG BufferREACHDanishdanishreach
MPG BufferREACHFinnishfinnishreach
MPG BufferREACHFrenchfrenchreach
MPG BufferREACHGermangermanreach
MPG BufferREACHItalianitalianreach
MPG BufferREACHNorwegiannorwegianreach
MPG BufferREACHSpanishspanishreach
MPG BufferREACHSwedishswedishreach
MPG BufferWHMSEnglishenglishwhms
MPG BufferWHMSFrenchfrenchwhms
MPX BufferREACHItalianitalianreach
MPX BufferGHSSpanishspanishghs
MPX BufferREACHGermangermanreach
MPX BufferREACHFrenchfrenchreach
MPX BufferREACHDanishdanishreach
MPX BufferREACHEnglishenglishreach
MPX BufferGHSEnglishenglishghs
MPX BufferREACHFinnishfinnishreach
MPX BufferREACHNorwegiannorwegianreach
MPX BufferREACHSpanishspanishreach
MPX BufferREACHSwedishswedishreach
MPX BufferWHMSEnglishenglishwhms
MPX BufferWHMSFrenchfrenchwhms
SPW BufferREACHGermangermanreach
SPW BufferREACHHungarianhungarianreach
SPW BufferREACHFrenchfrenchreach
SPW BufferREACHEnglishenglishreach
SPW BufferREACHDutchdutchreach
SPW BufferREACHDanishdanishreach
SPW BufferREACHItalianitalianreach
SPW BufferREACHNorwegiannorwegianreach
SPW BufferREACHPortugueseportuguesereach
SPW BufferREACHSpanishspanishreach
SPW BufferREACHSwedishswedishreach
SPW BufferGHSEnglishenglishghs
SPW BufferGHSSpanishspanishghs
SPW BufferWHMSEnglishenglishwhms
SPW BufferWHMSFrenchfrenchwhms
Elution BufferREACHGreekgreekreach
SPW BufferREACHGreekgreekreach
SPW BufferREACHFinnishfinnishreach
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