E.Z.N.A.® Micro RNA Kit


Isolation of miRNA & large RNA in separate fractions using spin columns

The E.Z.N.A.® Micro RNA Kit combines the reversible binding properties of the HiBind® matrix, a new silica-based material, with the highly efficient lysis ability of RNA-Solv® to extract micro and large (>200 nt) RNA from a wide variety of starting materials. Micro and large RNA are separated to allow for quantification into downstream applications.

The system uses RNA-Solv® to efficiently lyse cells and protect RNA from degradation. Following a chloroform separation step, RNA is applied to a HiBind® RNA column where large RNA binds. The flow-through is retained and applied to a HiBind® Micro RNA column to bind small RNA.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationRTPCR*, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation
Elution Volume15-30 μL for miRNA; 40-70 μL for >200 nt
Starting MaterialCultured eukaryotic cells or bacteria, or from animal,
plant, or fungal tissues
Starting AmountUp to 100 mg
RNA YieldUp to 50 µg
Processing ModeSpin column
Throughput1 – 24
RNA Binding TechnologySilica technology
Binding Capacity100 µg for each
HiBind® Micro RNA Column
HiBind® RNA Mini Column
2 mL Collection TubesView Product
RNA-Solv®View Product
RNA Wash Buffer IView Product
RNA Wash Buffer IIView Product
Nuclease-free Water

Figure 1. Purified large RNAs and miRNA from tissue samples was isolated with the E.Z.N.A.® miRNA Isolation Kit. RNA was extracted from samples as follows: sample 1 from fish liver, sample 2 from frog liver. Large RNA (5% of total purified RNA) was analyzed on a 1% agarose gel. miRNA (5% of total purified RNA) was analyzed on a 3% agarose gel to demonstrate yield and quality of the RNA.

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Protocol Manual
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Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
Nuclease-free WaterGHSEnglishenglishghs
Nuclease-free WaterGHSSpanishspanishghs
Nuclease-free WaterREACHEnglishenglishreach
Nuclease-free WaterREACHDanishdanishreach
Nuclease-free WaterREACHFinnishfinnishreach
Nuclease-free WaterREACHFrenchfrenchreach
Nuclease-free WaterREACHGermangermanreach
Nuclease-free WaterREACHItalianitalianreach
Nuclease-free WaterREACHNorwegiannorwegianreach
Nuclease-free WaterREACHSpanishspanishreach
Nuclease-free WaterREACHSwedishswedishreach
Nuclease-free WaterREACHDutchdutchreach
Nuclease-free WaterREACHPortugueseportuguesereach
Nuclease-free WaterREACHHungarianhungarianreach
Nuclease-free WaterWHMSFrenchfrenchwhms
Nuclease-free WaterWHMSEnglishenglishwhms
RNA Solv ReagentGHSEnglishenglishghs
RNA Solv ReagentGHSSpanishspanishghs
RNA Solv ReagentREACHEnglishenglishreach
RNA Solv ReagentREACHDanishdanishreach
RNA Solv ReagentREACHFinnishfinnishreach
RNA Solv ReagentREACHFrenchfrenchreach
RNA Solv ReagentREACHGermangermanreach
RNA Solv ReagentREACHItalianitalianreach
RNA Solv ReagentWHMSFrenchfrenchwhms
RNA Wash Buffer IGHSEnglishenglishghs
RNA Solv ReagentWHMSEnglishenglishwhms
RNA Solv ReagentREACHSpanishspanishreach
RNA Solv ReagentREACHNorwegiannorwegianreach
RNA Solv ReagentREACHSwedishswedishreach
RNA Wash Buffer IGHSSpanishspanishghs
RNA Wash Buffer IREACHEnglishenglishreach
RNA Wash Buffer IREACHDanishdanishreach
RNA Wash Buffer IREACHFinnishfinnishreach
RNA Wash Buffer IREACHFrenchfrenchreach
RNA Wash Buffer IREACHGermangermanreach
RNA Wash Buffer IREACHItalianitalianreach
RNA Wash Buffer IREACHNorwegiannorwegianreach
RNA Wash Buffer IWHMSFrenchfrenchwhms
RNA Wash Buffer IIGHSEnglishenglishghs
RNA Wash Buffer IWHMSEnglishenglishwhms
RNA Wash Buffer IREACHSwedishswedishreach
RNA Wash Buffer IREACHSpanishspanishreach
RNA Wash Buffer IIGHSSpanishspanishghs
RNA Wash Buffer IIREACHEnglishenglishreach
RNA Wash Buffer IIREACHDanishdanishreach
RNA Wash Buffer IIREACHFinnishfinnishreach
RNA Wash Buffer IIREACHFrenchfrenchreach
RNA Wash Buffer IIREACHGermangermanreach
RNA Wash Buffer IIREACHItalianitalianreach
RNA Wash Buffer IIREACHNorwegiannorwegianreach
RNA Wash Buffer IIREACHSpanishspanishreach
RNA Wash Buffer IIREACHSwedishswedishreach
RNA Wash Buffer IIWHMSEnglishenglishwhms
RNA Wash Buffer IIWHMSFrenchfrenchwhms
Nuclease-free WaterREACHGreekgreekreach
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