E.Z.N.A.® MicroElute® Cycle Pure Kit

  • Elution volumes – 10-15µl
  • Rapid – Purification of PCR products in 10 minutes
  • Convenient – No phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – DNA is suitable for downstream applications

This product has been replaced with the MicroElute® Cycle-Pure & Gel Extraction Kit.

This product is currently out of stock and unavailable.

Modified from the E.Z.N.A.® Cycle Pure Kit, the E.Z.N.A.® MicroElute® Cycle Pure Kit is specially designed to purify PCR samples with a small elution volume of 10-15 µL. Highly concentrated DNA eluates are ready to use in all subsequent applications.

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Downstream applicationCloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
Elution volume10-15 µL
Starting materialPCR reaction
Starting amountUp to 10 µg DNA
DNA recovered>90% recovery, 70 bp to 20 kb
Processing modeManual, centrifugation/vacuum
Throughput1
DNA binding technologySilica MicroElute spin column
Binding capacity10 μg
Processing time<15 minutes
Special noteLow elution volume

Figure 1.  Effect of elution volume on concentration and recovery using the MicroElute® Cycle Pure Kit. Elution volume versus recovery and DNA yield; 11 µg of 3 kb PCR product was purified using the MicroElute® Cycle Pure protocol and eluted with the indicated volume of elution buffer. The standard protocol uses 15 µL elution buffer because this combines higher yield and higher concentration. The yield can be increased by increasing the elution volume.

Product Information

Document NameLanguagesLink
Protocol ManualEnglish
Quick GuideEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
CP BufferGHSSpanishspanishghs
CP BufferGHSEnglishenglishghs
CP BufferWHMSFrenchfrenchwhms
CP BufferWHMSEnglishenglishwhms
CP BufferREACHSwedishswedishreach
CP BufferREACHSpanishspanishreach
CP BufferREACHNorwegiannorwegianreach
CP BufferREACHItalianitalianreach
CP BufferREACHGermangermanreach
CP BufferREACHFrenchfrenchreach
CP BufferREACHFinnishfinnishreach
CP BufferREACHDanishdanishreach
CP BufferREACHEnglishenglishreach
DNA Wash BufferWHMSFrenchfrenchwhms
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferREACHGermangermanreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHDutchdutchreach
Elution BufferWHMSFrenchfrenchwhms
Elution BufferWHMSEnglishenglishwhms
Elution BufferREACHSwedishswedishreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHGermangermanreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHEnglishenglishreach
Elution BufferGHSSpanishspanishghs
Elution BufferGHSEnglishenglishghs
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