E.Z.N.A.® MicroElute® Total RNA Kit


Isolates RNA from small samples with lower elution volumes using spin columns

E.Z.N.A.® MicroElute® Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as laser dissected samples (LDS) or fine-needle aspirates (FNA). Normally, up to 5 x 105 eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows the processing of single or multiple samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10-15 µL nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, poly A+ purification, nuclease protection and in vitro translation.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationPCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume15-20 μL
Starting MaterialCultured eukaryotic cells and soft tissues
Starting AmountUp to 5 x 105 eukaryotic cells or 5 mg tissue
RNA YieldUp to 50 µg
Processing ModeManual, centrifugation
Throughput1 – 24
RNA Binding TechnologySilica Micro spin column
Binding Capacity50 µg
MicroElute® LE RNA ColumnsView Product
2 mL Collection TubesView Product
TRK Lysis BufferView Product
RWF Wash Buffer
RNA Wash Buffer IIView Product
Nuclease-free Water
Carrier RNA

High Yield RNA

Figure 1. Yield of total RNA purified using the E.Z.N.A.® MicroElute® Total RNA Kit. Purified total RNA from 16 samples of 0.5 mg of fish liver was isolated with the E.Z.N.A.® MicroElute® Total RNA Kit. RNA was eluted in 20 µL DEPC-treated water and quantitated using Thermo Fisher’s NanoDrop® 2000.

Gel Electrophoresis

Figure 2. Total RNA purified using the E.Z.N.A.® MicroElute® Total RNA Kit. Purified total RNA from 0.2 mg chicken liver was isolated with the E.Z.N.A.® MicroElute® Total RNA Kit. Total RNA (100 ng/lane) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA.

Product Manuals

Protocol Manual
Quick Guide
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Product Information

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Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
Carrier RNAGHSSpanishspanishghs
Carrier RNAGHSEnglishenglishghs
Carrier RNAREACHPortugueseportuguesereach
Carrier RNAREACHHungarianhungarianreach
Carrier RNAREACHDutchdutchreach
Carrier RNAWHMSFrenchfrenchwhms
Carrier RNAWHMSEnglishenglishwhms
Carrier RNAREACHSwedishswedishreach
Carrier RNAREACHSpanishspanishreach
Carrier RNAREACHNorwegiannorwegianreach
Carrier RNAREACHItalianitalianreach
Carrier RNAREACHGermangermanreach
Carrier RNAREACHFrenchfrenchreach
Carrier RNAREACHFinnishfinnishreach
Carrier RNAREACHDanishdanishreach
Carrier RNAREACHEnglishenglishreach
Nuclease-free WaterREACHDanishdanishreach
Nuclease-free WaterREACHPortugueseportuguesereach
Nuclease-free WaterREACHHungarianhungarianreach
Nuclease-free WaterREACHDutchdutchreach
Nuclease-free WaterWHMSFrenchfrenchwhms
Nuclease-free WaterWHMSEnglishenglishwhms
Nuclease-free WaterREACHSwedishswedishreach
Nuclease-free WaterREACHSpanishspanishreach
Nuclease-free WaterREACHNorwegiannorwegianreach
Nuclease-free WaterREACHItalianitalianreach
Nuclease-free WaterREACHGermangermanreach
Nuclease-free WaterREACHFrenchfrenchreach
Nuclease-free WaterREACHFinnishfinnishreach
Nuclease-free WaterREACHEnglishenglishreach
Nuclease-free WaterGHSSpanishspanishghs
Nuclease-free WaterGHSEnglishenglishghs
RNA Wash Buffer IREACHSwedishswedishreach
RNA Wash Buffer IWHMSFrenchfrenchwhms
RNA Wash Buffer IWHMSEnglishenglishwhms
RNA Wash Buffer IREACHSpanishspanishreach
RNA Wash Buffer IREACHNorwegiannorwegianreach
RNA Wash Buffer IREACHItalianitalianreach
RNA Wash Buffer IREACHFrenchfrenchreach
RNA Wash Buffer IREACHFinnishfinnishreach
RNA Wash Buffer IREACHDanishdanishreach
RNA Wash Buffer IREACHEnglishenglishreach
RNA Wash Buffer IGHSSpanishspanishghs
RNA Wash Buffer IGHSEnglishenglishghs
RNA Wash Buffer IREACHGermangermanreach
RNA Wash Buffer IIREACHGermangermanreach
RNA Wash Buffer IIWHMSFrenchfrenchwhms
RNA Wash Buffer IIWHMSEnglishenglishwhms
RNA Wash Buffer IIREACHSwedishswedishreach
RNA Wash Buffer IIREACHSpanishspanishreach
RNA Wash Buffer IIREACHNorwegiannorwegianreach
RNA Wash Buffer IIREACHItalianitalianreach
RNA Wash Buffer IIREACHFrenchfrenchreach
RNA Wash Buffer IIREACHFinnishfinnishreach
RNA Wash Buffer IIREACHDanishdanishreach
RNA Wash Buffer IIREACHEnglishenglishreach
RNA Wash Buffer IIGHSSpanishspanishghs
RNA Wash Buffer IIGHSEnglishenglishghs
RWF BufferREACHDanishdanishreach
RWF BufferWHMSFrenchfrenchwhms
RWF BufferWHMSEnglishenglishwhms
RWF BufferREACHSwedishswedishreach
RWF BufferREACHSpanishspanishreach
RWF BufferREACHNorwegiannorwegianreach
RWF BufferREACHItalianitalianreach
RWF BufferREACHFrenchfrenchreach
RWF BufferREACHFinnishfinnishreach
RWF BufferREACHEnglishenglishreach
RWF BufferGHSSpanishspanishghs
RWF BufferGHSEnglishenglishghs
RWF BufferREACHGermangermanreach
TRK Lysis BufferWHMSFrenchfrenchwhms
TRK Lysis BufferWHMSEnglishenglishwhms
TRK Lysis BufferREACHSwedishswedishreach
TRK Lysis BufferREACHSpanishspanishreach
TRK Lysis BufferREACHNorwegiannorwegianreach
TRK Lysis BufferREACHItalianitalianreach
TRK Lysis BufferREACHGermangermanreach
TRK Lysis BufferREACHFrenchfrenchreach
TRK Lysis BufferREACHFinnishfinnishreach
TRK Lysis BufferREACHDanishdanishreach
TRK Lysis BufferREACHEnglishenglishreach
TRK Lysis BufferGHSSpanishspanishghs
TRK Lysis BufferGHSEnglishenglishghs
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