E.Z.N.A.® Plant & Fungal DNA Kit

$0.00$558.80

Isolate DNA from plant or fungal samples using spin columns.

  • Rapid – DNA isolation under 60 minutes
  • Reliable – Optimized buffer system ensure the reproducible results
  • Safe – No organic extractions
  • High-quality – Purified DNA suitable for most downstream applications

The E.Z.N.A.® Plant & Fungal DNA Kit allows for the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of plant or fungal species and tissues. Up to 200 mg wet tissue (or 50 mg dry tissue) can be processed in less than 1 hour. The
system combines the reversible nucleic acid-binding properties of Omega Bio-tek’s HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant and fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.

If using the E.Z.N.A.® Plant & Fungal DNA Kit for the first time, please read this booklet to become familiar with the procedure and its various modifications. Dry or fresh plant or fungal tissue is disrupted and lysed in a specially formulated buffer containing detergent. Proteins, polysaccharides, and cellular debris are subsequently precipitated. Contaminants are further removed by an isopropanol precipitation step. Binding conditions are adjusted and the sample is transferred to a HiBind® DNA Mini Column. Two rapid wash steps remove trace contaminants such as residual polysaccharides and pure DNA is eluted in water or low ionic strength buffer.

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Starting AmountUp to 50 mg wet tissue
Starting MaterialFresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content
Elution Volume50-100 μL
TechnologyHiBind® DNA Mini Column
Processing ModeManual
Throughput1-24
NoteSDS lysis

Product Information

NameDocumentLanguagesLink
Quick GuideProduct ManualEnglish
Protocol ManualProduct ManualEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferWHMSFrenchfrenchwhms
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHEnglishenglishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHDutchdutchreach
Elution BufferWHMSFrenchfrenchwhms
Elution BufferWHMSEnglishenglishwhms
Elution BufferREACHSwedishswedishreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHGermangermanreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHEnglishenglishreach
Elution BufferGHSSpanishspanishghs
Elution BufferGHSEnglishenglishghs
P1 BufferWHMSEnglishenglishwhms
P1 BufferWHMSFrenchfrenchwhms
P1 BufferREACHSwedishswedishreach
P1 BufferREACHSpanishspanishreach
P1 BufferREACHNorwegiannorwegianreach
P1 BufferREACHItalianitalianreach
P1 BufferREACHFrenchfrenchreach
P1 BufferREACHFinnishfinnishreach
P1 BufferREACHDanishdanishreach
P1 BufferREACHEnglishenglishreach
P1 BufferGHSSpanishspanishghs
P1 BufferGHSEnglishenglishghs
P1 BufferREACHGermangermanreach
P2 BufferREACHGermangermanreach
P2 BufferWHMSFrenchfrenchwhms
P2 BufferWHMSEnglishenglishwhms
P2 BufferREACHSwedishswedishreach
P2 BufferREACHSpanishspanishreach
P2 BufferREACHNorwegiannorwegianreach
P2 BufferREACHItalianitalianreach
P2 BufferREACHFrenchfrenchreach
P2 BufferREACHFinnishfinnishreach
P2 BufferREACHDanishdanishreach
P2 BufferREACHEnglishenglishreach
P2 BufferGHSSpanishspanishghs
P2 BufferGHSEnglishenglishghs
P3 BufferREACHDanishdanishreach
P3 BufferWHMSFrenchfrenchwhms
P3 BufferWHMSEnglishenglishwhms
P3 BufferREACHSwedishswedishreach
P3 BufferREACHSpanishspanishreach
P3 BufferREACHNorwegiannorwegianreach
P3 BufferREACHItalianitalianreach
P3 BufferREACHFrenchfrenchreach
P3 BufferREACHFinnishfinnishreach
P3 BufferREACHEnglishenglishreach
P3 BufferGHSSpanishspanishghs
P3 BufferGHSEnglishenglishghs
P3 BufferREACHGermangermanreach
RNase AWHMSFrenchfrenchwhms
RNase AWHMSEnglishenglishwhms
RNase AREACHSwedishswedishreach
RNase AREACHSpanishspanishreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHItalianitalianreach
RNase AREACHGermangermanreach
RNase AREACHFrenchfrenchreach
RNase AREACHFinnishfinnishreach
RNase AREACHDanishdanishreach
RNase AREACHEnglishenglishreach
RNase AGHSSpanishspanishghs
RNase AGHSEnglishenglishghs
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