E.Z.N.A.® Plasmid DNA Mini Kit I, (V-spin)


  • Rapid – Purification of plasmid DNA in less than 30 minutes
  • Safe – No Phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – DNA is suitable for a variety of downstream applications

The E.Z.N.A.® Plasmid DNA Mini Kit I is designed to isolate up to 25 µg of high-quality plasmid DNA from 1-5 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with HiBind® Mini Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.

The E.Z.N.A.® Plasmid DNA Mini Kit is available in two formats: Q-spin (D6942) and V-spin (D6943). D6942 (Q-spin) features columns that are capless, whereas D6943 (V-spin) includes columns that have a cap attached. The columns are otherwise identical in use and application and can be used in both vacuum or centrifugation protocols.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationCloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material1-5 mL LB culture
Plasmid typeHigh-copy, low-copy, cosmid DNA
Processing modeManual (centrifugation or vacuum)
DNA binding technologySilica Mini Spin Column
Lysate clearance methodCentrifugation
Processing time<30 minutes
Yield15-25 µg for high copy-number; 0.1-5 µg for low copy-number
Special noteThe alternative for discontinued yeast plasmid kit
HiBind® DNA Mini ColumnsView Product
2 mL Collection TubesView Product
Solution IView Product
Solution IIView Product
Solution IIIView Product
HBC BufferView Product
DNA Wash BufferView Product
RNase AView Product
Elution BufferView Product

Concentration Comparison

Figure 1.   pGEM plasmid was purified from 4 mL DH5α cultures harboring the plasmid and eluted in 50 µL volume using kits from Omega Bio-tek and Company Q according to manufacturer’s recommended protocols. Plasmid DNA concentration was determined by optical density measurements using Thermo Scientific’s NanoDrop™ 2000c system.

Super-Coiled DNA

Figure 2.   5 µL of purified pGEM plasmid was analyzed on a 1% Agarose gel. Plasmid was isolated from 1 mL DH5α cultures harboring the plasmid using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Mini Kit I.

Yield and Quality

Table 1.  pGEM plasmid was purified from 1 mL DH5α cultures harboring the plasmid and eluted in 50 µL volume. Plasmid DNA concentration was determined by optical density measurements using Thermo Scientific’s NanoDrop™ 2000c system.

Endotoxin Level

Table 2.  Plasmid DNA purified with E.Z.N.A. Plasmid DNA Mini Kit I was used in a 5 µL Sanger sequencing reaction.  DNA was analyzed on an Applied Biosystems 3730XL.

Table 3.  Endotoxins in plasmid DNA preps.  Plasmid DNA was isolated from 0.8 mL LB cultures following each manufacturer’s recommended protocols. Endotoxin levels were determined with Thermo Scientific’s Pierce LAL Chromogenic Endotoxin Quantitation Kit.

Product Information

Document NameLanguagesLink
E.Z.N.A® Plasmid DNA Mini Kit IEnglish
Protocol ManualEnglish
Quick GuideEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
Solution IREACHSwedishswedishreach
Solution IGHSEnglishenglishghs
Solution IWHMSFrenchfrenchwhms
Solution IWHMSEnglishenglishwhms
Solution IREACHSpanishspanishreach
Solution IREACHNorwegiannorwegianreach
Solution IREACHItalianitalianreach
Solution IREACHGermangermanreach
Solution IREACHFrenchfrenchreach
Solution IREACHFinnishfinnishreach
Solution IREACHDanishdanishreach
Solution IREACHEnglishenglishreach
Solution IGHSSpanishspanishghs
Solution IIREACHGermangermanreach
Solution IIWHMSFrenchfrenchwhms
Solution IIWHMSEnglishenglishwhms
Solution IIREACHSwedishswedishreach
Solution IIREACHSpanishspanishreach
Solution IIREACHNorwegiannorwegianreach
Solution IIREACHItalianitalianreach
Solution IIREACHFinnishfinnishreach
Solution IIREACHDanishdanishreach
Solution IIREACHEnglishenglishreach
Solution IIGHSSpanishspanishghs
Solution IIGHSEnglishenglishghs
Solution IIREACHFrenchfrenchreach
Solution IIIWHMSFrenchfrenchwhms
Solution IIIWHMSEnglishenglishwhms
Solution IIIREACHSwedishswedishreach
Solution IIIREACHSpanishspanishreach
Solution IIIREACHNorwegiannorwegianreach
Solution IIIREACHItalianitalianreach
Solution IIIREACHGermangermanreach
Solution IIIREACHFrenchfrenchreach
Solution IIIREACHFinnishfinnishreach
Solution IIIREACHDanishdanishreach
Solution IIIREACHEnglishenglishreach
Solution IIIGHSSpanishspanishghs
Solution IIIGHSEnglishenglishghs
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