E.Z.N.A.® Total RNA Kit II


Isolates total cellular RNA from fatty tissues with organic solvents using spin column

The E.Z.N.A.® Total RNA Kit II is designed for isolating total cellular RNA from tissues rich in fat, such as brain adipose tissues. However, this kit can also be used for the isolation of total RNA from other types of tissues including cultured eukaryotic cells, animal tissues, or bacteria.

The E.Z.N.A.® Total RNA Kit II uses the reversible binding properties of HiBind® matrix, a new silica-based material. By combining the high lysis efficiency of RNA-Solv® Reagent with Omega Bio-tek’s innovative HiBind® technology, this kit can extract total cellular RNA from all types of animal or human tissues including fatty tissues such as brain and adipose tissue. A specifically formulated high salt buffer system allows more than 100 µg RNA molecules greater than 200 bases to bind to the matrix. Cells or tissues are first homogenized with RNA-Solv® Reagent that inactivates RNases. After adding chloroform, the homogenate is separated into an aqueous and organic phase by centrifugation. The aqueous phase which contains the RNA is adjusted with ethanol and applied to the HiBind® RNA Mini Column to which total RNA binds, while cellular debris and other contaminants are washed away. High-quality RNA is eluted in Nuclease-Free Water.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationPCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume45-75 μL
Starting MaterialTissues rich in fat
Starting Amount1 x 107 eukaryotic cells or 25-30 mg tissue
RNA YieldUp to 100 µg
Processing ModeManual, centrifugation/vacuum
Throughput1 – 24
RNA Binding TechnologySilica Mini spin column
Binding Capacity100 µg
HiBind® RNA Mini Columns
2 mL Collection TubesView Product
RNA-Solv® ReagentView Product
RNA Wash Buffer IView Product
RNA Wash Buffer IIView Product
Nuclease-free Water

Yield and Quality

Figure 1. Total RNA purified using the E.Z.N.A.® Total RNA Kit II. Purified total RNA from mouse tissue samples was isolated with the E.Z.N.A.® Total RNA Kit II. Total RNA (300 ng/lane) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA. M: CL5000 DNA Marker.

RT-PCR Template

Figure 2. RNA was isolated from 10 mg chicken liver using the E.Z.N.A.® Total RNA Kit II. A serial dilution of recovered RNA was then used in a SYBR® Green ral-time RT-PCR targeting a 150 bp fragment of the β-actin. Each reaction was performed in 40 replicates. The lines represent input RNA amounts of 50, 5, 0.5, 0.05 and 0.005 ng (left to right) per reaction.

Product Manuals

Protocol Manual
1 document

Product Information

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Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
Nuclease-free WaterGHSSpanishspanishghs
Nuclease-free WaterREACHGreekgreekreach
Nuclease-free WaterREACHPortugueseportuguesereach
Nuclease-free WaterREACHHungarianhungarianreach
Nuclease-free WaterREACHDutchdutchreach
Nuclease-free WaterWHMSFrenchfrenchwhms
Nuclease-free WaterWHMSEnglishenglishwhms
Nuclease-free WaterREACHSwedishswedishreach
Nuclease-free WaterREACHSpanishspanishreach
Nuclease-free WaterREACHItalianitalianreach
Nuclease-free WaterGHSEnglishenglishghs
Nuclease-free WaterREACHEnglishenglishreach
Nuclease-free WaterREACHDanishdanishreach
Nuclease-free WaterREACHFrenchfrenchreach
Nuclease-free WaterREACHNorwegiannorwegianreach
Nuclease-free WaterREACHGermangermanreach
Nuclease-free WaterREACHFinnishfinnishreach
RNA Solv ReagentGHSEnglishenglishghs
RNA Solv ReagentWHMSFrenchfrenchwhms
RNA Solv ReagentWHMSEnglishenglishwhms
RNA Solv ReagentREACHSwedishswedishreach
RNA Solv ReagentREACHSpanishspanishreach
RNA Solv ReagentREACHNorwegiannorwegianreach
RNA Solv ReagentREACHGermangermanreach
RNA Solv ReagentGHSSpanishspanishghs
RNA Solv ReagentREACHEnglishenglishreach
RNA Solv ReagentREACHDanishdanishreach
RNA Solv ReagentREACHFrenchfrenchreach
RNA Solv ReagentREACHItalianitalianreach
RNA Solv ReagentREACHFinnishfinnishreach
RNA Wash Buffer IGHSSpanishspanishghs
RNA Wash Buffer IWHMSFrenchfrenchwhms
RNA Wash Buffer IWHMSEnglishenglishwhms
RNA Wash Buffer IREACHSwedishswedishreach
RNA Wash Buffer IREACHSpanishspanishreach
RNA Wash Buffer IREACHNorwegiannorwegianreach
RNA Wash Buffer IREACHGermangermanreach
RNA Wash Buffer IGHSEnglishenglishghs
RNA Wash Buffer IREACHEnglishenglishreach
RNA Wash Buffer IREACHDanishdanishreach
RNA Wash Buffer IREACHFinnishfinnishreach
RNA Wash Buffer IREACHFrenchfrenchreach
RNA Wash Buffer IREACHItalianitalianreach
RNA Wash Buffer IIGHSEnglishenglishghs
RNA Wash Buffer IIGHSSpanishspanishghs
RNA Wash Buffer IIREACHEnglishenglishreach
RNA Wash Buffer IIREACHDanishdanishreach
RNA Wash Buffer IIREACHFinnishfinnishreach
RNA Wash Buffer IIREACHFrenchfrenchreach
RNA Wash Buffer IIREACHGermangermanreach
RNA Wash Buffer IIREACHItalianitalianreach
RNA Wash Buffer IIREACHNorwegiannorwegianreach
RNA Wash Buffer IIREACHSpanishspanishreach
RNA Wash Buffer IIREACHSwedishswedishreach
RNA Wash Buffer IIWHMSEnglishenglishwhms
RNA Wash Buffer IIWHMSFrenchfrenchwhms
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