E.Z.N.A.® Universal Pathogen Kit
$0.00 – $231.50
• Elute in as low as 15 μL
• Prefilled Disruptor Tubes for faster sample homogenization
• No Phenol/chloroform extractions
• High-quality DNA suitable for a variety of downstream applications
The E.Z.N.A. Universal Pathogen DNA Kit allows for rapid and reliable isolation of high-quality host genomic DNA, gram-positive and gram-negative bacterial DNA, fungal spore DNA, yeast DNA, viral DNA and viral RNA from tissues, blood, urine, serum, whole blood, and stool samples.
This kit incorporates Omega Bio-tek’s Disruptor Tubes prefilled with glass beads for faster and easier processing. The Disruptor Tubes allow for simultaneous homogenization and lysis of the samples and aid in the effective disruption of difficult samples. No detergents are present in the initial lysis buffer, which eliminates foaming issues and provides optimal conditions for homogenization.
This unique buffer system does not require alcohol to bind nucleic acids, allowing for the recovery of high-quality DNA/RNA free of PCR inhibitors. Omega Bio-tek’s MicroElute LE Spin Columns are used, which support elution volumes as low as 15 μL to obtain a high concentration of the eluate.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Starting Amount | 25-30 mg tissue, 250 µL fecal sample / serum / urine/blood, |
| Starting Material | tissue, urine, serum, and fecal sample |
| Elution Volume | 15-100 μL |
| Technology | Silica MicroElute spin column |
| Processing Mode | Manual, Centrifugation/Vacuum |
| Note | Host genomic DNA, gram positive and negative bacterial DNA, fungal spore DNA, and viral DNA and RNA |
| ITEM | AVAILABLE SEPARATELY |
|---|---|
| Disruptor Tubes | View Product |
| MicroElute® LE DNA Columns | View Product |
| 2 mL Collection Tubes | — |
| SLX-Mlus Buffer | View Product |
| DS Buffer | Call for Pricing |
| PCP Buffer | — |
| RBB Buffer | View Product |
| HBC Buffer | View Product |
| DNA Wash Buffer | View Product |
| Elution Buffer | View Product |
| Proteinase K Solution | View Product |
Figure 1. Human fecal samples suspended in PBS solution were spiked into corresponding organisms. Fecal samples were then processed according to each manufacturer’s recommended protocols. qPCR was performed in triplicate for each sample using primers specific for the target organism. Data shown are averages on triplicate reactions.