E.Z.N.A.® Viral RNA Kit
$23.50 – $679.60
Isolate viral RNA from cell-free fluids such as plasma, serum, urine, and cell culture supernatant using spin columns.
• Viral RNA/DNA purification from wide variety of sample types
• Can be used to clean-up samples in Trizol
• No organic extractions
The E.Z.N.A. Viral RNA Kit is designed to isolate viral RNA and DNA from plasma, serum, cell culture supernatant and urine, swabs, and saliva. The procedure completely removes contaminants and enzyme inhibitors, making viral RNA isolation fast, convenient, and reliable. The purified nucleic acids are ready for direct use in downstream applications such as qPCR, RT-qPCR and more.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Starting Amount | 150 µL |
| Starting Material | Cell free fluids such as plasma, serum, urine and cell culture supernatant |
| Elution Volume | 20-50 μL |
| Technology | HiBind® RNA Mini Column |
| Processing Mode | Manual, Centrifugation/Vacuum |
| Throughput | 1-24 |
| ITEM | AVAILABLE SEPARATELY |
|---|---|
| HiBind® RNA Mini Columns | — |
| 2 mL Collection Tubes | View Product |
| QVL Lysis Buffer | View Product |
| RNA Wash Buffer II | View Product |
| VHB Buffer | View Product |
| Carrier RNA (Poly A) | — |
| Nuclease-Free water | View Product |
Figure 1. 25 µL of Zeptomatrix Influenza A/B Positive Control was spiked into 125 µL serum samples and then Viral RNA was extracted using the E.Z.N.A.® Viral RNA Kit . 2 µL of Eluted RNA was used as a template in a 20 µL SYBR® qPCR reaction. C: inhibitor-free control; T: RNA sample extracted with kit. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template RNA in free of inhibitors.
FAQs
Can the E.Z.N.A. Viral RNA Kit isolate DNA viruses as well?
Yes, this kit can be used to isolate DNA and RNA from viruses in cell-free fluids.
How do I reconstitute the Carrier RNA provided in the E.Z.N.A. Viral RNA Kit?
The instructions for reconstituting the Carrier RNA are provided in the “Preparing Reagents” section of the manual on Page 5.
For a 5-prep kit, add 50 µL of Nuclease-Free Water to the tube of lyophilized Carrier RNA, shake to dissolve completely, aliquot, and store at -20 °C.
For a 50-prep kit, add 320 µL of Nuclease-Free Water to the tube of lyophilized Carrier RNA, shake to dissolve completely, aliquot, and store at -20 °C.
Do not freeze/ thaw the aliquots more than 3 times.