E.Z.N.A.® Tissue DNA Kit


Isolate DNA from tissues, buccal swabs, cultured cells, whole blood, body fluids, paraffin-embedded tissues and mouse tail snips using mini spin columns

  • Rapid – DNA isolation in less than 20 minutes post-lysis
  • Versatile – Single kit for multiple sample types
  • Specialized buffer system – Optimized buffers for higher yields
  • Safe – No phenol/chloroform extractions
  • High-quality – DNA is suitable for a variety of downstream applications

The E.Z.N.A. Tissue DNA Kit offers a versatile and cost-effective method for isolating DNA from a wide variety of samples, including fresh or frozen animal cultured cells and tissues, buccal swabs, whole blood, mouse tail snips, etc. The DNA purification process is simplified with Silica Mini Spin Column technology into four quick lyse, bind, wash, and elute steps and can be accomplished in less than 20 minutes post-lysis. This convenient spin-column format avoids time-consuming steps like alcohol precipitation and the use of toxic compounds such as phenol and chloroform and allows for multiple samples to be processed in parallel. DNA purified using this kit is ready for most downstream applications such as PCR, sequencing, genotyping, southern blot analysis, and restriction enzyme digestion.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationsPCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion
Starting MaterialTissues, cultured cells, mouse tail snips, paraffin-embedded tissues, whole blood, body fluids, buccal swabs
Starting Amount30 mg, or 5 x 106 cultured cells
Elution Volume100-200 μL
DNA Binding TechnologySilica mini spin column
Processing Time< 20 min (post-lysis)
HiBind® DNA Mini ColumnsView Product
2 mL Collection TubesView Product
BL BufferView Product
TL BufferView Product
HBC BufferView Product
DNA Wash BufferView Product
Elution BufferView Product
Proteinase K SolutionView Product

Size Comparison

Figure 1.  Purified genomic DNA from 10 mg rat kidney tissue was isolated using kits from Company T, Company A, Company P, Company Q, and Omega Bio-tek’s E.Z.N.A.® Tissue DNA Kit following manufacturer’s recommended protocols. 3% of eluted DNA was analyzed on a 0.8% agarose gel. M:Lambda-Hind III.

qPCR Analysis

Figure 2.  Genomic DNA was isolated from 10 mg of rat kidney with Omega Bio-tek’s E.Z.N.A.® Tissue DNA Kit. Serial dilutions of recovered genomic DNA were used as templates for real-time PCR amplification of a 100 bp fragment of the GAPDH gene with SYBR® Green labeling. Each reaction was performed in triplicate. The fluorescence versus cycle number is plotted and the 5 curves correspond to the input DNA template amounts of 10, 2, 0.4, 0.08, and 0.0016 ng.

Yield & Quality

Table 1.  Genomic DNA was extracted from 30 mg mouse liver using the E.Z.N.A. Tissue DNA Kit Protocol for Tissue samples and eluted in 100 µL volume. DNA concentration was determined by optical density measurements using Thermo Scientific’s NanoDrop ™ 2000c system.

Size & Quality Analysis

Figure 3.  Genomic DNA was extracted from 30 mg mouse liver using the E.Z.N.A. Tissue DNA Kit Protocol for Tissue samples and eluted in 100 µL volume. 5 µL eluate was analyzed on a 1% Agarose gel.

PCR Analysis

Figure 4.  Genomic DNA was extracted from 30 mg mouse liver using the E.Z.N.A. Tissue DNA Kit Protocol for Tissue samples. 2 µL of Eluted DNA was diluted 10- and 100-fold and used as a template in a 20 µL SYBR® qPCR reaction. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template DNA in free of inhibitors.

Can the E.Z.N.A. Tissue DNA Kit extract DNA from hair?

Yes. For hair, please add DTT to a final concentration of 40 mM to the lysis buffer (TL) and extend incubation at 55 °C as needed.

What extraction kit do you recommend for extracting DNA from yeast?

We recommend D3396 with protocol modifications.

The modification will require making or sourcing Sorbitol buffer (1 M sorbitol; 100 mM sodium EDTA; 14 mM β-mercaptoethanol) and obtaining lyticase. Before beginning the tissue DNA protocol on page 8 of the E.Z.N.A. Tissue DNA Kit, you will need to perform the steps outlined below.

  1. Harvest cells (maximum 2 x 107) by centrifuging for 10 minutes at 5,000 x g (approximately 7,500 rpm). Discard the supernatant.
  2. Resuspend the pellet in 600 µL sorbitol buffer and 200 units of lyticase. Incubate at 30 °C for 30 minutes.
  3. Pellet the spheroplasts by centrifuging for 10 minutes at 300 x g.

Continue on using step 2 of the Tissue DNA protocol found on page 8 of the E.Z.N.A. Tissue DNA Kit product manual.

Product Information

Quick GuideProduct ManualEnglish
Protocol ManualProduct ManualEnglish
E.Z.N.A. Tissue DNA KitProduct LiteratureEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
BL BufferGHSEnglishenglishghs
BL BufferGHSSpanishspanishghs
BL BufferREACHEnglishenglishreach
BL BufferREACHDanishdanishreach
BL BufferREACHFinnishfinnishreach
BL BufferREACHFrenchfrenchreach
BL BufferREACHGermangermanreach
BL BufferREACHItalianitalianreach
BL BufferREACHNorwegiannorwegianreach
BL BufferREACHSpanishspanishreach
BL BufferREACHSwedishswedishreach
BL BufferWHMSFrenchfrenchwhms
BL BufferWHMSEnglishenglishwhms
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferWHMSFrenchfrenchwhms
DNA Wash BufferWHMSEnglishenglishwhms
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSwedishswedishreach
Elution BufferREACHSpanishspanishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
HBC BufferGHSEnglishenglishghs
HBC BufferGHSSpanishspanishghs
HBC BufferREACHEnglishenglishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHFinnishfinnishreach
HBC BufferREACHGermangermanreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHSpanishspanishreach
HBC BufferREACHSwedishswedishreach
HBC BufferWHMSEnglishenglishwhms
HBC BufferWHMSFrenchfrenchwhms
Proteinase K SolutionGHSEnglishenglishghs
Proteinase K SolutionGHSSpanishspanishghs
Proteinase K SolutionREACHDanishdanishreach
Proteinase K SolutionREACHEnglishenglishreach
Proteinase K SolutionREACHFinnishfinnishreach
Proteinase K SolutionREACHFrenchfrenchreach
Proteinase K SolutionREACHGermangermanreach
Proteinase K SolutionREACHItalianitalianreach
Proteinase K SolutionREACHNorwegiannorwegianreach
Proteinase K SolutionREACHSpanishspanishreach
Proteinase K SolutionREACHSwedishswedishreach
Proteinase K SolutionREACHDutchdutchreach
Proteinase K SolutionWHMSEnglishenglishwhms
Proteinase K SolutionWHMSFrenchfrenchwhms
Proteinase K SolutionREACHHungarianhungarianreach
Proteinase K SolutionREACHPortugueseportuguesereach
TL BufferGHSEnglishenglishghs
TL BufferGHSSpanishspanishghs
TL BufferREACHEnglishenglishreach
TL BufferREACHDanishdanishreach
TL BufferREACHFinnishfinnishreach
TL BufferREACHItalianitalianreach
TL BufferREACHSwedishswedishreach
TL BufferREACHSpanishspanishreach
TL BufferREACHNorwegiannorwegianreach
TL BufferREACHGermangermanreach
TL BufferREACHFrenchfrenchreach
TL BufferWHMSEnglishenglishwhms
TL BufferWHMSFrenchfrenchwhms
TL BufferREACHDutchdutchreach
TL BufferREACHHungarianhungarianreach
TL BufferREACHPortugueseportuguesereach
Elution BufferREACHGreekgreekreach
Proteinase K SolutionREACHGreekgreekreach
TL BufferREACHGreekgreekreach
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