Mag-Bind® cfDNA Kit

The Mag-Bind® cfDNA Kit is designed for the rapid and efficient isolation of circulating cell-free DNA from up to 10 mL plasma or serum samples. The Mag-Bind cfDNA Kit can be processed manually or using automated platforms. The procedure eliminates the need for funnels and vacuum steps, providing hands-free operation in automated protocols.

The unique formulation of the lysis and binding buffers allows complete automation of the extraction process for up to 10 mL sample volumes with minimal user intervention. The magnetic properties of the Mag-Bind Particles CH enable fast magnetic separation, even when using large volumes. The high binding capacity of the beads allows for a lower volume of magnetic particles needed, thus reducing the final elution volume required. 10 mL of serum or plasma can be eluted in as low as 50 μL.

The system combines the reversible nucleic acid-binding properties of Mag-Bind paramagnetic particles with a unique binding system that targets smaller DNA fragments (150-400 bp) and minimizes the binding of larger fragments, such as gDNA.

This Kit can be automated on most open liquid handlers or magnetic processors. Ready-to-implement protocols are available for the following platforms:

• Dynamic Devices Lynx
• Hamilton Microlab® STAR
• Thermo Fisher Scientific KingFisher® Apex
• Thermo Fisher Scientific KingFisher® Flex