Mag-Bind® cfDNA Kit

$0.00$2,550.20

Rapid & efficient isolation of circulating, cell-free DNA from 0.5-10 mL plasma or serum samples

  • Isolate cell-free DNA (cfDNA) from up to 10 mL Plasma or Serum
  • Automation-ready scripts process 96 (1 mL) samples in 2 hours
  • High-binding magnetic beads allow 4 mL serum or plasma to be eluted in 50 µL

CE-IVD marked kit is available: Mag-Bind® cfDNA Kit CE IVD – (omegabiotek.com)

M3298-00S Mag-Bind® cfDNA Kit (Sample Request)

$0.00

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

The Mag-Bind® cfDNA Kit is designed for the rapid and efficient isolation of circulating cell-free DNA from up to 10 mL plasma or serum samples. The Mag-Bind cfDNA Kit can be processed manually or using automated platforms. The procedure eliminates the need for funnels and vacuum steps, providing hands-free operation in automated protocols.

The unique formulation of the lysis and binding buffers allows complete automation of the extraction process for up to 10 mL sample volumes with minimal user intervention. The magnetic properties of the Mag-Bind Particles CH enable fast magnetic separation, even when using large volumes. The high binding capacity of the beads allows for a lower volume of magnetic particles needed, thus reducing the final elution volume required. 10 mL of serum or plasma can be eluted in as low as 50 μL.

The system combines the reversible nucleic acid-binding properties of Mag-Bind paramagnetic particles with a unique binding system that targets smaller DNA fragments (150-400 bp) and minimizes the binding of larger fragments, such as gDNA.

This Kit can be automated on most open liquid handlers or magnetic processors. Ready-to-implement protocols are available for the following platforms:

  • Dynamic Devices Lynx
  • Hamilton Microlab® STAR
  • Thermo Fisher Scientific KingFisher® Apex
  • Thermo Fisher Scientific KingFisher® Flex

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Sample TypePlasma/Serum
Sample Volume500-10,000 µL
TechnologyMagnetic Beads
ProcessingManual or Automated
AutomatableYes
Processing Time2 hours for 96 (1mL) samples
FormatTube, 24-well, 96-well
Elution Volume50 µL
ITEMAVAILABLE SEPARATELY
Mag-Bind® Particles CHCall for Pricing
DS BufferCall for Pricing
JSB BufferView Product
GT7 Buffer v1.1Call for Pricing
SPW BufferView Product
Elution BufferView Product
Proteinase K SolutionView Product

Little Genomic Carryover

Figure 1. Electropherogram overlay of purified DNA from 4 mL serum. 4 mL of unspiked serum was purified using kits from Omega Bio-tek and Company Q following manufacturer’s recommended protocols. Purified DNA was analyzed on Agilent’s TapeStation® 2200.

High Yield and Quality

Figure 2.  Electropherogram overlay of purified DNA from 1 mL serum. Sheared, bacterial DNA was spiked into serum samples and isolated using Omega Bio-tek’s Mag-Bind cfDNA Kit and a column-based kit from Company Q. Real-time PCR was performed using 16s bacteria-specific primers at 1X and 10X dilutions. Purified DNA was analyzed on Agilent’s TapeStation® 2200. The Omega Bio-tek kit was able to capture the circulating, cell-free DNA with no genomic DNA contamination. In contrast, Company Q’s eluate contained high molecular weight fragments indicating the presence of genomic DNA in the circulating DNA isolation.

No PCR Inhibitors

Table 1.  Real-time PCR with 16S bacterial-specific primers was performed on triplicates of undiluted and 10-fold dilutions of DNA isolated from samples purified [figure 2]. The data shows the purified DNA is free of PCR inhibitors and the specific cfDNA concentration is higher than Company Q.

Recover Fragments as Small as 50 bp

Figure 3. DNA fragments as small as 50 bp can be recovered using modified protocol. Serum spiked with 50 bp DNA ladder was purified with the Mag-Bind® cfDNA Kit. By using modified protocols, smaller fragments were recovered.

TapeStation Analysis

Figure 4. TapeStation® analysis of cfDNA purified from 10 mL of unspiked plasma samples (n = 12) using Omega Bio-tek’s kit automated on Hamilton Microlab® STAR™ platform.

Table 2. Gel image from figure 4.1 was analyzed using the TapeStation software. Peak and concentration data showed that 8 mL automation process was a success.

Figure 5. Gel image from figure 4.1 was analyzed using the TapeStation software. Peak and concentration data showed that 8 mL automation process was a success.

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