Mag-Bind® cfDNA LSP Kit
$1,680.00
Pre-scripted, prefilled, fully automated solution for extracting cfDNA from 4mL of plasma or serum!
- Easy-to-use – Reagents and buffers ready-to-run in automation-ready, prefilled, barcoded reservoirs and tubes.
- Reduce User Error – Barcoded reservoirs and tubes identify reagent and buffer positions for the pre-scripted protocol.
- High-speed, high-quality purification – 96 (4 plates) 4mL samples eluted into as little as 50 μL in under 3.5 hours.
SKU#: PS3298-1-96PF (4 x 24 preps)
The Mag-Bind® cfDNA LSP Kit is designed for the efficient and reliable purification of circulating cell-free DNA (cfDNA) from plasma or serum samples, with the capability to process input volumes of up to 4 ml per sample in one to four 24-well plates with 50 to 200 µl elution volumes. This kit is automation-ready, featuring prefilled and barcoded reagent reservoirs and tubes for seamless integration with most open ended liquid handling platforms, ensuring traceability, consistency, and high-quality results across large sample sets.
This workflow offers a fully automated solution for processing cfDNA, after samples have been transferred to sample plates, reducing hands-on time while improving extraction efficiency and accuracy. The prefilled reagents eliminate the need for preparation and dispensing steps, enhancing ease of use. Barcoded reagent reservoirs and tubes are scannable to allow the instrument to recognize reagent placement on liquid handler decks.
The magnetic particle-based purification system ensures fast and efficient binding, washing, and elution of cfDNA, even when processing larger sample volumes. Up to 4 ml of plasma/serum can be eluted in 50-200 µl of elution buffer. The purified cfDNA is of high quality, suitable for immediate use in downstream applications such as PCR, qPCR, digital PCR, and next-generation sequencing (NGS).
| Feature | Specifications |
|---|---|
| Sample Type | Plasma/Serum |
| Sample Volume | 4 mL |
| Technology | Magnetic Beads |
| Processing | Automated |
| Processing Time | ~ 3.5 hrs (4 x 24 samples) |
| Elution Volume | 50 - 200 uL |
| Reservoir Material | Polypropylene |
| Reservoir Height | 44 mm |
| Footprint | ANSI/SLAS Compliant |
| For use with (equipment) | Hamilton Microlab® STAR™ |
| SKU No | PS3298-1-96PF | Available Separately |
|---|---|---|
| Purifications | 4 x 24 | |
| 24-well Sample Plates | 4 | |
| 96-well Tip Isolator Plates | 4 | |
| DS Buffer | 2 x 14.5 mL | |
| GT7 Buffer v1.1 | 222 mL | GT7 Buffer |
| JSB Buffer | 2 x 222 mL | JSB Buffer |
| eSPW Buffer | 222 mL | |
| Elution Buffer | 2 x 180 mL | Elution Buffer |
| Mag-Bind® Particles CH | 2 x 1.4 mL | Mag-Bind® Particles CH |
| Proteinase K Solution | 2 x 3.4 mL | Proteinase K |
*Components available separately are not delivered in pre-filled reservoirs or tubes.
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Pre-scripted Solution on a Hamilton Microlab® STAR™
4 Plate Method: Average cfDNA Concentration
cfDNA was extracted from 4 mL pooled plasma and eluted in 100 μL. 4 x 24 samples processed in ~3 hrs 20 min on a Hamilton Microlab® STAR. A) TapeStation analysis of cfDNA extracted with the Mag-Bind® cfDNA LSP Kit shows well-defined cfDNA bands ~180 bp. B) Concentration in 50-700 bp range and average yield are shown.
4 Plate Method: Well-defined Bands of cfDNA
2 Plate Method: Average cfDNA Concentration
cfDNA was extracted from 4 mL pooled plasma and eluted in 100 μL. 2 x 24 samples processed in ~2 hrs 45 min. on a Hamilton Microlab® STAR. A) TapeStation analysis of cfDNA extracted with the Mag-Bind® cfDNA LSP Kit shows well-defined cfDNA bands ~180 bp. B) Concentration in 50-700 bp range and average yield are shown.
2 Plate Method: Well-defined Bands of cfDNA
1 Plate Method: Average cfDNA Concentration
cfDNA was extracted from 4 mL pooled plasma and eluted in 200 μL. 1 x 24 samples processed in ~2.5 hrs on a Hamilton Microlab® STAR™. A) TapeStation analysis of cfDNA extracted with the Mag-Bind® cfDNA LSP Kit shows well-defined cfDNA bands ~180 bp. B) Concentration in 50-700 bp range and average yield are shown.
1 Plate Method: Well-defined Bands of cfDNA
