Mag-Bind® Ultra-Pure Plasmid DNA 96 Kit


Purify endotoxin-free plasmid DNA (<0.1 EU/µg) using magnetic beads

  • Bead-based, scalable DNA purification
  • Manual and automation friendly
  • Cost-effective – Approximately 30% less than competition

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

The Mag-Bind® family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a variety of sources. Key to the system is Omega Bio-tek’s proprietary Mag-Bind® Particle that avidly, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer.

The Mag-Bind® Ultra-Pure Plasmid DNA 96 Kit combines the power of Mag-Bind® technology with the innovative ETR technology (Endo-toxin removal) to deliver high-quality endotoxin-free plasmid DNA in high throughput format. Yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 1 mL of overnight culture in LB medium typically produces 10-15 μg for high-copy plasmid. The purified plasmid can be used directly for automated fluorescent DNA sequencing, such as with BigDye® sequencing chemistry, transfection as well as for other standard molecular biology techniques including restriction enzyme digestion.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationSensitive cell line transfection; cloning; sanger sequencing etc.
Starting materialPer well: 1.0-1.5 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid typeHigh-copy, low-copy, cosmid DNA
Processing modeAutomated, Magnetic processing
Throughput96 samples per run
DNA binding technologyMagnetic beads
Lysate clearance methodCentrifugation, Magnetic Beads or 96-well Filter Plate
Yield10-15 μg for high copy-number plasmid
Mag-Bind® Particles RQCall for Pricing
Solution IView Product
Solution IIView Product
N3 Buffer
ETR Binding BufferView Product
ETR Wash Buffer 
VHB BufferView Product
SPM Wash BufferView Product
Elution BufferView Product
RNase AView Product

Yield and Quality

Figure 1.  9 samples of 1 mL E. Coli culture transformed with pGEM vector were purified using the Mag-Bind® Ultra-Pure Plasmid Kit. The elution volume was 100 µL. The DNA concentration was measured with Nanodrop 2000c.

Super-Coiled DNA

Figure 2.  1 mL DH5α cultures transformed with pGEM vector were isolated according to the standard protocol. 5 µL eluate plasmid DNA was analyzed on a 1% Agarose gel.

Sanger Sequencing

Figure 3.  Plasmid DNA purified with Mag-Bind Ultra-Pure Plasmid DNA Kit was used in a 5 µL Sanger sequencing reaction. DNA was analyzed on an Applied Biosystems 3730XL.

Endotoxin Level

Figure 4.  Endotoxins in plasmid DNA preps. Plasmid DNA was isolated from 0.8 mL LB cultures following each manufacturer’s recommended protocols. Endotoxin levels were determined with Thermo Scientific’s Pierce LAL Chromogenic Endotoxin Quantitation Kit.

Product Information

Document NameLanguagesLink
Protocol ManualEnglish
Mag-Bind® Ultra-Pure Plasmid DNA KitEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
ETR Binding BufferGHSEnglishenglishghs
ETR Binding BufferGHSSpanishspanishghs
ETR Binding BufferREACHEnglishenglishreach
ETR Binding BufferREACHDanishdanishreach
ETR Binding BufferREACHFinnishfinnishreach
ETR Binding BufferREACHFrenchfrenchreach
ETR Binding BufferREACHGermangermanreach
ETR Binding BufferREACHItalianitalianreach
ETR Binding BufferREACHNorwegiannorwegianreach
ETR Binding BufferREACHSpanishspanishreach
ETR Binding BufferREACHSwedishswedishreach
ETR Binding BufferWHMSEnglishenglishwhms
ETR Binding BufferWHMSFrenchfrenchwhms
ETR Wash BufferGHSEnglishenglishghs
ETR Wash BufferGHSSpanishspanishghs
ETR Wash BufferREACHEnglishenglishreach
ETR Wash BufferREACHDanishdanishreach
ETR Wash BufferREACHFinnishfinnishreach
ETR Wash BufferREACHFrenchfrenchreach
ETR Wash BufferREACHGermangermanreach
ETR Wash BufferREACHItalianitalianreach
ETR Wash BufferREACHNorwegiannorwegianreach
ETR Wash BufferREACHSpanishspanishreach
ETR Wash BufferREACHSwedishswedishreach
ETR Wash BufferWHMSEnglishenglishwhms
ETR Wash BufferWHMSFrenchfrenchwhms
Mag-Bind Particles RQGHSEnglishenglishghs
Mag-Bind Particles RQGHSSpanishspanishghs
Mag-Bind Particles RQREACHEnglishenglishreach
Mag-Bind Particles RQREACHDanishdanishreach
Mag-Bind Particles RQREACHFinnishfinnishreach
Mag-Bind Particles RQREACHFrenchfrenchreach
Mag-Bind Particles RQREACHGermangermanreach
Mag-Bind Particles RQREACHItalianitalianreach
Mag-Bind Particles RQREACHNorwegiannorwegianreach
Mag-Bind Particles RQREACHSpanishspanishreach
Mag-Bind Particles RQREACHSwedishswedishreach
Mag-Bind Particles RQWHMSEnglishenglishwhms
Mag-Bind Particles RQWHMSFrenchfrenchwhms
Mag-Bind Particles RQREACHDutchdutchreach
Mag-Bind Particles RQREACHHungarianhungarianreach
Mag-Bind Particles RQREACHPortugueseportuguesereach
N3 BufferGHSEnglishenglishghs
N3 BufferGHSSpanishspanishghs
N3 BufferREACHEnglishenglishreach
N3 BufferREACHDanishdanishreach
N3 BufferREACHFinnishfinnishreach
N3 BufferREACHFrenchfrenchreach
N3 BufferREACHGermangermanreach
N3 BufferREACHItalianitalianreach
N3 BufferREACHNorwegiannorwegianreach
N3 BufferREACHSpanishspanishreach
N3 BufferREACHSwedishswedishreach
N3 BufferWHMSEnglishenglishwhms
N3 BufferWHMSFrenchfrenchwhms
RNase AGHSEnglishenglishghs
RNase AGHSSpanishspanishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFinnishfinnishreach
RNase AREACHFrenchfrenchreach
RNase AREACHGermangermanreach
RNase AREACHItalianitalianreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHSpanishspanishreach
RNase AREACHSwedishswedishreach
RNase AWHMSEnglishenglishwhms
RNase AWHMSFrenchfrenchwhms
Solution IGHSEnglishenglishghs
Solution IGHSSpanishspanishghs
Solution IREACHEnglishenglishreach
Solution IREACHDanishdanishreach
Solution IREACHFinnishfinnishreach
Solution IREACHFrenchfrenchreach
Solution IREACHGermangermanreach
Solution IREACHItalianitalianreach
Solution IREACHNorwegiannorwegianreach
Solution IREACHSpanishspanishreach
Solution IREACHSwedishswedishreach
Solution IWHMSEnglishenglishwhms
Solution IWHMSFrenchfrenchwhms
Solution IIGHSEnglishenglishghs
Solution IIGHSSpanishspanishghs
Solution IIREACHEnglishenglishreach
Solution IIREACHDanishdanishreach
Solution IIREACHFinnishfinnishreach
Solution IIREACHFrenchfrenchreach
Solution IIREACHGermangermanreach
Solution IIREACHItalianitalianreach
Solution IIREACHNorwegiannorwegianreach
Solution IIREACHSpanishspanishreach
Solution IIREACHSwedishswedishreach
Solution IIWHMSEnglishenglishwhms
Solution IIWHMSFrenchfrenchwhms
SPM BufferGHSEnglishenglishghs
SPM BufferGHSSpanishspanishghs
SPM BufferREACHEnglishenglishreach
SPM BufferREACHDanishdanishreach
SPM BufferREACHFinnishfinnishreach
SPM BufferREACHFrenchfrenchreach
SPM BufferREACHGermangermanreach
SPM BufferREACHItalianitalianreach
SPM BufferREACHNorwegiannorwegianreach
SPM BufferREACHSpanishspanishreach
SPM BufferREACHSwedishswedishreach
SPM BufferWHMSEnglishenglishwhms
SPM BufferWHMSFrenchfrenchwhms
SPM BufferREACHDutchdutchreach
SPM BufferREACHHungarianhungarianreach
SPM BufferREACHPortugueseportuguesereach
VHB BufferGHSEnglishenglishghs
VHB BufferGHSSpanishspanishghs
VHB BufferREACHEnglishenglishreach
VHB BufferREACHDanishdanishreach
VHB BufferREACHFinnishfinnishreach
VHB BufferREACHFrenchfrenchreach
VHB BufferREACHGermangermanreach
VHB BufferREACHItalianitalianreach
VHB BufferREACHNorwegiannorwegianreach
VHB BufferREACHSpanishspanishreach
VHB BufferREACHSwedishswedishreach
VHB BufferWHMSEnglishenglishwhms
VHB BufferWHMSFrenchfrenchwhms
VHB BufferREACHDutchdutchreach
VHB BufferREACHHungarianhungarianreach
VHB BufferREACHPortugueseportuguesereach
Elution BufferREACHGreekgreekreach
Mag-Bind Particles RQREACHGreekgreekreach
SPM BufferREACHGreekgreekreach
VHB BufferREACHGreekgreekreach
Shopping Cart
Scroll to Top