$279.10 – $2,503.80
Isolate viral DNA & RNA from whole blood, serum, plasma & bodily fluids using magnetic beads.
All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.
Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.
Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.
The Mag-Bind® Viral DNA/RNA Kit is designed for rapid and reliable isolation of viral DNA and RNA from cell-free samples such as serum, plasma, cell culture supernatant, and other biological samples such as swabs, aspirates, etc. The Mag-Bind® paramagnetic bead technology is optimized for the recovery of low viral titer and provides high-quality viral DNA or RNA suitable for direct use in most downstream applications such as RT-PCR, PCR, and other enzymatic reactions. The extraction methodology is easily adaptable to various automated systems and can be scaled up or down depending on the amount of starting material used. The kit is not designed to separate cellular DNA from viral nucleic acids and cellular nucleic acid will be co-purified if present.
Protocols are available for the following automated platforms:
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Starting Amount | 50 µL – 200 µL |
| Starting Material | Serum, plasma, saliva and other body fluids |
| Elution Volume | 20-50 μL |
| Technology | Magnetic Beads |
| Processing Mode | Automated, Manual |
| Throughput | 8 – 96 |
| Mag-Bind® Particles CNR | Call for Pricing |
| TNA Lysis Buffer | — |
| VHB Buffer | View Product |
| Carrier RNA | — |
| Proteinase K Solution (40 mg/mL) | Call for Pricing |
| SPR Wash Buffer | — |
| Nuclease-free Water | View Product |
Figure 1. HBV virus (in quantities of 10 and 1 infectious unit[s]) was spiked into 200 µL human serum. Viral nucleic acid was isolated with Omega Bio-tek’s Mag-Bind® Viral DNA/RNA Kit and with a comparable kit from Company A according to recommended protocols. 5 µL of template was used for a SYBR® Green-labeled qPCR reaction which was replicated 4 times. The resulting mean Ct values are shown in the above figure.
Figure 2. Nucleic acid was isolated from 200 µL of human whole blood with Omega Bio-tek’s Mag-Bind® Viral DNA/RNA Kit and a comparable kit from Company A using the manufacturer’s recommended protocols. The extractions were eluted in 100 µL. Three concentrations of template were used as templates in a SYBR® Green-labeled qPCR reaction. Each reaction was performed in quadruplicate and the mean Ct value is depicted in the above figure.
Figure 3. 50 µL of ZeptoMetrix’s NATtrol Influenza A/B Positive Control standard was spiked into 150 µL of human serum and viral nucleic acids were extracted using Omega Bio-tek’s Mag-Bind® Viral DNA/RNA 96 Kit. Average Ct values obtained after amplification using Influenza B primers are shown above. The results indicate positive detection of Influenza B in both undiluted and 10-fold diluted purified samples.
