E.Z.N.A.® Cycle Pure Kit (V-spin)


  • Rapid – Purification of PCR products in less than 10 minutes
  • Safe – No Phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – DNA is suitable for a variety of downstream applications

The E.Z.N.A.® Cycle Pure Kit is a convenient system for the fast and reliable purification of PCR products. The E.Z.N.A.® Cycle Pure Kit uses HiBind® technology to recover DNA bands from 100 bp to 10 kb free of oligonucleotides, nucleotides, and polymerase with yields exceeding 80%. The binding conditions of the HiBind® DNA Mini Columns are adjusted by the addition of a specially formulated buffer before adding the sample. Following a rapid wash step, DNA is eluted with deionized water or a low salt buffer. Purified DNA can be directly used for most downstream applications include T-A ligations, PCR sequencing, restriction enzyme digestion, or various labeling reactions.

For Research Use Only. Not for use in diagnostic procedures.

Downstream applicationT-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions
Elution volume30-50 µL
Starting materialssDNA, dsDNA, PCR products
Starting amount5-50 µL
DNA recovered>80% recovery, 100 bp to 10 kb
Processing modeManual (centrifugation or vacuum)
DNA binding technologySilica mini spin column
Processing time<10 minutes
Special NotesFor clean-up of DNA fragments < 200 bp, please follow the protocol modification outlined in the product manual
HiBind® DNA Mini ColumnsView Product
2 mL Collection TubesView Product
CP BufferView Product
Elution BufferView Product
DNA Wash BufferView Product


Gel Electrophoresis Analysis

Figure 1.  500 bp and 5 kb DNA fragments were cleaned up with products from Company T, Company A, Company P, Company Q and Omega Bio-tek following manufaturer’s recommended protocols. DNA was analyzed on a 0.8% agarose gel.

Sanger Sequencing

Figure 2.  500 bp amplicon was purified with the E.Z.N.A. Cycle Pure Kit was used in a 5 µL Sanger sequencing reaction. DNA was analyzed on an Applied Biosystem 3730XL.

What are the minimum and maximum size products that can be isolated with the E.Z.N.A. Cycle Pure Kit?

The E.Z.N.A. Cycle Pure Kit can bind DNA between 100 bp – 10 kbp. The protocol mentions, “for PCR products smaller than 200 bp, add 5 volumes CP Buffer and 0.4 volumes 100% isopropanol”. If you follow this modification, the minimum bp size that the columns would bind would be 50bp.

What is the binding capacity of the column in D6492?

30 µg

Product Information

Document NameLanguagesLink
Quick GuideEnglish
Protocol ManualEnglish
E.Z.N.A® Cycle Pure KitEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
CP BufferGHSSpanishspanishghs
CP BufferGHSEnglishenglishghs
CP BufferREACHFinnishfinnishreach
CP BufferREACHEnglishenglishreach
CP BufferREACHDanishdanishreach
CP BufferREACHFrenchfrenchreach
CP BufferREACHGermangermanreach
CP BufferREACHItalianitalianreach
CP BufferREACHNorwegiannorwegianreach
CP BufferREACHSpanishspanishreach
CP BufferREACHSwedishswedishreach
CP BufferWHMSEnglishenglishwhms
CP BufferWHMSFrenchfrenchwhms
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferWHMSFrenchfrenchwhms
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHGermangermanreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHGreekgreekreach
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