Mag-Bind® Plant DNA DS 96 Kit

$0.00$651.50

Isolate DNA from plant samples using magnetic beads.

  • Straightforward, rapid, and reliable procedure
  • Adaptable in most robotic liquid handling platform

The Mag-Bind® Plant DNA DS 96 Kit allows rapid and reliable isolation of high-quality genomic DNA from plants and other tissues that are particularly difficult to lyse or very high in polysaccharide content. The lysis and binding buffers are specifically designed to minimize co-purification of polysaccharides and polyphenols. Up 96 samples of 50 mg wet tissue (or 15 mg dry tissue) can be processed in parallel in less than one hour. The system combines Omega Bio-tek’s E.Z.N.A.® buffer chemistry with the convenience of Mag-Bind® Particles to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. Purified DNA is suitable for PCR, restriction digestion, Next Generation Sequencing, and hybridization applications. There are no organic extractions thereby reducing plastic waste and decreasing hands-on time to allow multiple samples to be processed in parallel.

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Starting Amount50 mg wet tissue or 15 mg dry tissue
Starting MaterialPlants and other tissues that are particularly difficult to lyse or very high in polysaccharide content.
Elution Volume100-200 μL
TechnologyMagnetic Beads
Processing ModeAutomated, Manual
Throughput96
ITEMAVAILABLE SEPARATELY
CSPL BufferView Product
RBB BufferView Product
CSPW1 BufferView Product
CSPW2 BufferView Product
SPM BufferView Product
Elution BufferView Product
Proteinase K SolutionView Product
RNase A (25 mg/mL)View Product
Mag-Bind® Particles HDQCall for Pricing

DNA Yield Comparison

Figure 1. DNA was extracted from approximately 50 mg of leaf sample using Omega Bio-tek’s Mag-Bind® Plant DNA DS 96 Kit (M1130) and Company Q’s kit following manufacturer’s recommended protocols. Purified DNA was quantified using Promega’s QuantiFluor® dsDNA system and normalized per mg of input plant material.

Molecular Weight Analysis

Figure 2  TapeStation® analysis of genomic DNA purified from barley using Omega Bio-tek’s Mag-Bind® Plant DNA DS 96 kit (M1130). TapeStation® analysis software estimates the molecular weight of the purified DNA to be ~30kb.

Yield and Quality

Figure 3.1  Genomic DNA was purified from 50 mg canola leaf with the Mag-Bind Plant DNA DS 96 Kit. DNA concentration determined by optical density measurements with NanoDrop® 2000c. Total elution volume was 100 µL.

Size Analysis

Figure 3.2  Genomic DNA was purified from 50 mg canola leaf with the Mag-Bind Plant DNA DS 96 Kit. 5 µL eluate DNA was analyzed on a 1% Agarose gel.

Inhibitor-Free DNA

Figure 3.3  Genomic DNA was extracted from 30 mg canola leaf using the Mag-Bind Plant DNA DS 96 Kit (n=4). Real-time PCR with canola-specific primers was performed on triplicates of 10-fold and 100-fold dilutions of DNA. The ΔCt value between 100-fold and 10-fold was ~3.3 for all the samples, demonstrating good PCR efficiency without inhibition.

Is it possible to obtain a high molecular weight using the Mag-Bind Plant DNA DS 96 Kit?

It is challenging to extract high molecular weight DNA from plants. The pre-processing step with plants involves bead-beating, and bead-beating shears the DNA up. We recently did TapeStation® analysis on M1130 extracted plant DNA and found the size was roughly 20 kb.

What is the difference between M1128 and M1130?

The Mag-Bind Plant DNA DS 96 Kit (M1130) is an improved version of the Mag-Bind Plant DNA Plus 96 Kit (M1128). It has been reworked to better handle difficult samples (DS = Difficult Samples) that are rich in polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates.

I am getting low 260/230 absorbance ratios using the Mag-Bind Plant DNA DS 96 Kit. How can I improve this?

The low A260/A230 ratios indicate two things. First is chaotropic salt carryover from the extraction. The second is sample-related carryover (polyphenols, polysaccharides etc.). If it is salt carryover, the ratio can be bettered by increasing the number of wash steps (3 SPM washes instead of 2). If it is a contaminant from the sample itself, we would recommend adding 1% β -mercaptoethanol and freshly prepared 2% PVP to CSPL buffer. PVP forms complexes with polyphenols and allows them to be separated from nucleic acids. β-mercaptoethanol is a strong denaturing agent and can help get rid of the polysaccharides.

Product Information

NameDocumentLanguagesLink
Protocol ManualProduct ManualEnglish
Mag-Bind® Plant DNA DS 96 KitProduct LiteratureEnglish
Automated, High Throughput SNP Genotyping of Zea maysApplication NoteEnglish
Rapid, High Performance and Cost-Effective Plant DNA ExtractionsApplication NoteEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
CSPL BufferGHSEnglishenglishghs
CSPL BufferGHSSpanishspanishghs
CSPL BufferREACHEnglishenglishreach
CSPL BufferREACHDanishdanishreach
CSPL BufferREACHFinnishfinnishreach
CSPL BufferREACHFrenchfrenchreach
CSPL BufferREACHGermangermanreach
CSPL BufferREACHItalianitalianreach
CSPL BufferREACHNorwegiannorwegianreach
CSPL BufferREACHSpanishspanishreach
CSPL BufferREACHSwedishswedishreach
CSPL BufferWHMSEnglishenglishwhms
CSPL BufferWHMSFrenchfrenchwhms
CSPW1 BufferGHSEnglishenglishghs
CSPW1 BufferGHSSpanishspanishghs
CSPW1 BufferREACHEnglishenglishreach
CSPW1 BufferREACHDanishdanishreach
CSPW1 BufferREACHFinnishfinnishreach
CSPW1 BufferREACHFrenchfrenchreach
CSPW1 BufferREACHGermangermanreach
CSPW1 BufferREACHItalianitalianreach
CSPW1 BufferREACHNorwegiannorwegianreach
CSPW1 BufferREACHSpanishspanishreach
CSPW1 BufferREACHSwedishswedishreach
CSPW1 BufferWHMSEnglishenglishwhms
CSPW1 BufferWHMSFrenchfrenchwhms
CSPW2 BufferGHSEnglishenglishghs
CSPW2 BufferGHSSpanishspanishghs
CSPW2 BufferREACHEnglishenglishreach
CSPW2 BufferREACHDanishdanishreach
CSPW2 BufferREACHFinnishfinnishreach
CSPW2 BufferREACHFrenchfrenchreach
CSPW2 BufferREACHGermangermanreach
CSPW2 BufferREACHItalianitalianreach
CSPW2 BufferREACHNorwegiannorwegianreach
CSPW2 BufferREACHSpanishspanishreach
CSPW2 BufferREACHSwedishswedishreach
CSPW2 BufferWHMSEnglishenglishwhms
CSPW2 BufferWHMSFrenchfrenchwhms
Mag-Bind Particles HDQGHSEnglishenglishghs
Mag-Bind Particles HDQREACHEnglishenglishreach
Mag-Bind Particles HDQREACHDanishdanishreach
Mag-Bind Particles HDQREACHFinnishfinnishreach
Mag-Bind Particles HDQREACHFrenchfrenchreach
Mag-Bind Particles HDQREACHGermangermanreach
Mag-Bind Particles HDQREACHItalianitalianreach
Mag-Bind Particles HDQREACHNorwegiannorwegianreach
Mag-Bind Particles HDQREACHSpanishspanishreach
Mag-Bind Particles HDQREACHSwedishswedishreach
Mag-Bind Particles HDQWHMSEnglishenglishwhms
Mag-Bind Particles HDQWHMSFrenchfrenchwhms
Mag-Bind Particles HDQREACHDutchdutchreach
Mag-Bind Particles HDQREACHHungarianhungarianreach
Mag-Bind Particles HDQREACHPortugueseportuguesereach
Proteinase K SolutionGHSEnglishenglishghs
Proteinase K SolutionGHSSpanishspanishghs
Proteinase K SolutionREACHEnglishenglishreach
Proteinase K SolutionREACHDanishdanishreach
Proteinase K SolutionREACHFinnishfinnishreach
Proteinase K SolutionREACHFrenchfrenchreach
Proteinase K SolutionREACHGermangermanreach
Proteinase K SolutionREACHItalianitalianreach
Proteinase K SolutionREACHNorwegiannorwegianreach
Proteinase K SolutionREACHSpanishspanishreach
Proteinase K SolutionREACHSwedishswedishreach
Proteinase K SolutionWHMSEnglishenglishwhms
Proteinase K SolutionWHMSFrenchfrenchwhms
Proteinase K SolutionREACHDutchdutchreach
Proteinase K SolutionREACHHungarianhungarianreach
Proteinase K SolutionREACHPortugueseportuguesereach
RBB BufferGHSEnglishenglishghs
RBB BufferGHSSpanishspanishghs
RBB BufferREACHEnglishenglishreach
RBB BufferREACHDanishdanishreach
RBB BufferREACHFinnishfinnishreach
RBB BufferREACHFrenchfrenchreach
RBB BufferREACHGermangermanreach
RBB BufferREACHItalianitalianreach
RBB BufferREACHNorwegiannorwegianreach
RBB BufferREACHSpanishspanishreach
RBB BufferREACHSwedishswedishreach
RBB BufferWHMSEnglishenglishwhms
RBB BufferWHMSFrenchfrenchwhms
RNase AGHSEnglishenglishghs
RNase AGHSSpanishspanishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFinnishfinnishreach
RNase AREACHFrenchfrenchreach
RNase AREACHGermangermanreach
RNase AREACHItalianitalianreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHSpanishspanishreach
RNase AREACHSwedishswedishreach
RNase AWHMSEnglishenglishwhms
RNase AWHMSFrenchfrenchwhms
SPM BufferGHSEnglishenglishghs
SPM BufferGHSSpanishspanishghs
SPM BufferREACHEnglishenglishreach
SPM BufferREACHDanishdanishreach
SPM BufferREACHFinnishfinnishreach
SPM BufferREACHFrenchfrenchreach
SPM BufferREACHGermangermanreach
SPM BufferREACHItalianitalianreach
SPM BufferREACHNorwegiannorwegianreach
SPM BufferREACHSpanishspanishreach
SPM BufferREACHSwedishswedishreach
SPM BufferWHMSEnglishenglishwhms
SPM BufferWHMSFrenchfrenchwhms
SPM BufferREACHDutchdutchreach
SPM BufferREACHHungarianhungarianreach
SPM BufferREACHPortugueseportuguesereach
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