E.Z.N.A.® Plasmid DNA Mini Kit I, (Q – spin)


  • Rapid – Purification of plasmid DNA in less than 30 minutes
  • Safe – No Phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – DNA is suitable for a variety of downstream applications

The E.Z.N.A.® family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is the HiBind® matrix that specifically, but reversibly, binds DNA or RNA under optimized conditions allowing proteins and other contaminants to be removed. The E.Z.N.A.® Plasmid DNA Mini Kits combine the power of HiBind® technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA in less than 30 minutes. HiBind® DNA Mini Columns facilitate the binding, washing, and elution steps thus enabling multiple samples to be processed simultaneously.

The E.Z.N.A.® Plasmid DNA Mini Kit is available with two different types of columns. V-spin columns have an attached cap, while Q-spin columns are capless. The columns are otherwise identical in use and application. Both columns can be used with the vacuum or centrifugation protocol.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationCloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material1-5 mL LB culture
Plasmid typeHigh-copy, low-copy, cosmid DNA
Processing modeManual (centrifugation or vacuum)
DNA binding technologySilica Mini Spin Column
Lysate clearance methodCentrifugation
Processing time<30 minutes
Yield15-25 µg for high copy-number; 0.1-5 µg for low copy-number
HiBind® DNA Mini ColumnsView Product
2 mL Collection TubesView Product
Solution IView Product
Solution IIView Product
Solution IIIView Product
HBC BufferView Product
DNA Wash BufferView Product
RNase AView Product
Elution BufferView Product

Concentration Comparison

Figure 1.   pGEM plasmid was purified from 4 mL DH5α cultures harboring the plasmid and eluted in 50 µL volume using kits from Omega Bio-tek and Company Q according to manufacturer’s recommended protocols. Plasmid DNA concentration was determined by optical density measurements using Thermo Scientific’s NanoDrop™ 2000c system.

Super-Coiled DNA

Figure 2.   5 µL of purified pGEM plasmid was analyzed on a 1% Agarose gel. Plasmid was isolated from 1 mL DH5α cultures harboring the plasmid using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Mini Kit I.

Yield and Quality

Table 1.  pGEM plasmid was purified from 1 mL DH5α cultures harboring the plasmid and eluted in 50 µL volume. Plasmid DNA concentration was determined by optical density measurements using Thermo Scientific’s NanoDrop™ 2000c system.

Endotoxin Level

Table 2.  Plasmid DNA purified with E.Z.N.A. Plasmid DNA Mini Kit I was used in a 5 µL Sanger sequencing reaction.  DNA was analyzed on an Applied Biosystems 3730XL.

Table 3.  Endotoxins in plasmid DNA preps.  Plasmid DNA was isolated from 0.8 mL LB cultures following each manufacturer’s recommended protocols. Endotoxin levels were determined with Thermo Scientific’s Pierce LAL Chromogenic Endotoxin Quantitation Kit.

What is the final concentration of RNase in Solution I?

100 µg/mL

Can I isolate plasmid larger than 20 kb?

The kits work efficiently for plasmid sizes up to 20 kb. You will see reduced yields for plasmids >20 kb. The recommendations for recovery of plasmids >10 kb – heat the elution buffer to 7 ˚C, incubate the elution buffer on the column for 5 min and do a second elution with the eluate from the first round. We have seen success with recovery of 25 kb plasmids.

Are there any special storage conditions for the components of the plasmid kits?

Solution I (once RNase A is added) should be stored at 2-8 ˚C. All other materials should be stored at room temperature.

Can your plasmid kit work for yeast too?

Yes, following some protocol modifications. You will need to source sorbitol buffer and lyticase.

Please prepare fresh sorbitol buffer (1 M sorbitol; 100 mM sodium EDTA; 14 mM β-mercaptoethanol)

  1. Harvest cells (maximum 2 x 107) by centrifuging for 10 minutes at 5000 x g (approximately 7500 rpm). Discard the supernatant.
  2. Resuspend the pellet in 600 µL sorbitol buffer and 200 units of lyticase. Incubate at 30 ˚C for 30 minutes.
  3. Pellet the spheroblasts by centrifuging for 10 minutes at 300 x g.
  4. Continue from step 4 (page 10) of the E.Z.N.A.® Plasmid Mini Kit I manual.

Can the E.Z.N.A. Plasmid Mini Kit I clean-up already eluted plasmid?

Purified plasmid can be re-bound to the HiBind DNA column using HBC Buffer (5 volumes of HBC to 1 elution), followed by 2 rounds of wash with DNA Wash Buffer. The last step is to dry the column and elute the DNA. Note: some DNA will be lost due to the column’s inherent properties.

Product Information

Document NameLanguagesLink
E.Z.N.A® Plasmid DNA Mini Kit IEnglish
Protocol ManualEnglish
Quick GuideEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferWHMSFrenchfrenchwhms
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHEnglishenglishreach
Elution BufferREACHGreekgreekreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHDutchdutchreach
Elution BufferWHMSFrenchfrenchwhms
Elution BufferWHMSEnglishenglishwhms
Elution BufferREACHSwedishswedishreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHItalianitalianreach
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHGermangermanreach
Elution BufferREACHFinnishfinnishreach
HBC BufferGHSEnglishenglishghs
HBC BufferWHMSFrenchfrenchwhms
HBC BufferWHMSEnglishenglishwhms
HBC BufferREACHSwedishswedishreach
HBC BufferREACHSpanishspanishreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHGermangermanreach
HBC BufferGHSSpanishspanishghs
HBC BufferREACHEnglishenglishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHFinnishfinnishreach
RNase AGHSSpanishspanishghs
RNase AWHMSFrenchfrenchwhms
RNase AWHMSEnglishenglishwhms
RNase AREACHSwedishswedishreach
RNase AREACHSpanishspanishreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHGermangermanreach
RNase AGHSEnglishenglishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFrenchfrenchreach
RNase AREACHItalianitalianreach
RNase AREACHFinnishfinnishreach
Solution IGHSSpanishspanishghs
Solution IWHMSFrenchfrenchwhms
Solution IWHMSEnglishenglishwhms
Solution IREACHSwedishswedishreach
Solution IREACHSpanishspanishreach
Solution IREACHNorwegiannorwegianreach
Solution IREACHGermangermanreach
Solution IGHSEnglishenglishghs
Solution IREACHEnglishenglishreach
Solution IREACHDanishdanishreach
Solution IREACHFrenchfrenchreach
Solution IREACHItalianitalianreach
Solution IREACHFinnishfinnishreach
Solution IIREACHFinnishfinnishreach
Solution IIREACHNorwegiannorwegianreach
Solution IIREACHSwedishswedishreach
Solution IIREACHItalianitalianreach
Solution IIREACHDanishdanishreach
Solution IIREACHSpanishspanishreach
Solution IIGHSSpanishspanishghs
Solution IIREACHFrenchfrenchreach
Solution IIWHMSEnglishenglishwhms
Solution IIWHMSFrenchfrenchwhms
Solution IIREACHEnglishenglishreach
Solution IIGHSEnglishenglishghs
Solution IIREACHGermangermanreach
Solution IIIREACHEnglishenglishreach
Solution IIIREACHDanishdanishreach
Solution IIIREACHFinnishfinnishreach
Solution IIIREACHFrenchfrenchreach
Solution IIIREACHGermangermanreach
Solution IIIREACHItalianitalianreach
Solution IIIREACHNorwegiannorwegianreach
Solution IIIREACHSpanishspanishreach
Solution IIIREACHSwedishswedishreach
Solution IIIWHMSEnglishenglishwhms
Solution IIIWHMSFrenchfrenchwhms
Solution IIIGHSEnglishenglishghs
Solution IIIGHSSpanishspanishghs
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