Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit

$1,690.70

Features and Benefits

  • Ease of Use – Reduction of hands-on time with no reagent preparation (for bind, wash, and elute steps)
  • Convenience – Inclusion of tip combs and other required plasticware in the kit for user convenience
  • Extraction Accuracy – Reduction of user-associated errors
  • Safe – No organic solvents
  • High-Quality – Purified DNA suitable for downstream applications such as next-generation sequencing, qPCR, PCR, and microarrays

SKU#: M6399-01PF96 (4 x 96 Preps)

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

The Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit includes prefilled plates in 96-well standard automation format. The configuration of this kit works seamlessly on magnetic processors such as the Thermo Fisher Scientific KingFisher® Flex, QIAGEN BioSprint®, and Applied Biosystems MagMAX® Express-96. Included in this kit are all plastic accessories, including tip combs, that are needed for an extraction run post-lysis. This kit offers a versatile method for isolating high-quality DNA from a wide variety of samples, including fresh or frozen animal cultured cells and tissues, buccal swabs, mouse tail snips, dried blood spots, up to 250 μL whole blood, and up to 250 μL saliva.With no reagent set-up and the quick magnetic response of the Mag-Bind® Particles PF-HDQ, hands-on time and the overall processing time are significantly reduced. The purification procedure provides high-quality DNA suitable for direct use in most downstream applications including amplification, next generation sequencing (NGS), and enzymatic reactions.

Protocols are available for the following magnetic processors:

  • Thermo Fisher Scientific KingFisher® Flex
  • QIAGEN BioSprint®
  • Applied Biosystems MagMAX® Express-96

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Starting MaterialWhole blood, saliva, swabs, mouse tails, buffy coat, dried blood spots, cultured cells
Starting AmountVariable (depending on the sample input)
Elution Volume100 µL
Processing ModeAutomated, Post-lysis
Throughput4 x 96
Nucleic Acid Binding TechnologyMagnetic Beads
Run Time~40 minutes
For scripting support, contact one of our automation support specialists.
Downstream Application(s)qPCR, NGS, microarray, etc
Storage ConditionsStore all components at room temperature in an upright position.
Proteinase K Solution at 2-8°C

Table 1. The average DNA yield from 250 μL whole blood samples and 10 mg mouse kidney tissue samples were obtained using the Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit and eluted in 100 μL volume. DNA yield was determined using Thermo Scientific’s NanoDrop® 2000c. The absorbance ratios > 1.8 indicate high purity of the isolated DNA.

High Molecular Weight DNA

Figure 2. The size of genomic DNA purified from mouse kidney tissue samples using the Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit was analyzed on the Agilent TapeStation® 2200. The purified DNA is of high molecular weight, with the software analyzing it to be > 60 kb. The DIN values are all > 8.2, indicating highly intact DNA of superior quality.

Average Ct Values

Figure 3. Average Ct values obtained amplifying the purified DNA from blood and tissue samples using the Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit . The ΔCt value was ~3.3, indicating high-quality DNA that is free of inhibitors.

Product Information

NameDocumentLanguagesLink
Protocol ManualProduct ManualEnglish
Automated DNA Extraction Workflow Using Rhinostics’ HIPPOstic Swabs and Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 KitApplication NoteEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
AL BufferGHSEnglishenglishghs
AL BufferGHSSpanishspanishghs
AL BufferREACHEnglishenglishreach
AL BufferREACHDanishdanishreach
AL BufferREACHFinnishfinnishreach
AL BufferREACHFrenchfrenchreach
AL BufferREACHGermangermanreach
AL BufferREACHItalianitalianreach
AL BufferREACHNorwegiannorwegianreach
AL BufferREACHSpanishspanishreach
AL BufferREACHSwedishswedishreach
AL BufferWHMSEnglishenglishwhms
AL BufferWHMSFrenchfrenchwhms
AL BufferREACHDutchdutchreach
AL BufferREACHHungarianhungarianreach
AL BufferREACHPortugueseportuguesereach
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
eSPM BufferGHSEnglishenglishghs
eSPM BufferGHSSpanishspanishghs
eSPM BufferREACHEnglishenglishreach
eSPM BufferREACHDanishdanishreach
eSPM BufferREACHFinnishfinnishreach
eSPM BufferREACHFrenchfrenchreach
eSPM BufferREACHGermangermanreach
eSPM BufferREACHItalianitalianreach
eSPM BufferREACHNorwegiannorwegianreach
eSPM BufferREACHSpanishspanishreach
eSPM BufferREACHSwedishswedishreach
eSPM BufferWHMSEnglishenglishwhms
eSPM BufferWHMSFrenchfrenchwhms
eVHB BufferGHSEnglishenglishghs
eVHB BufferGHSSpanishspanishghs
eVHB BufferREACHEnglishenglishreach
eVHB BufferREACHDanishdanishreach
eVHB BufferREACHFinnishfinnishreach
eVHB BufferREACHFrenchfrenchreach
eVHB BufferREACHGermangermanreach
eVHB BufferREACHItalianitalianreach
eVHB BufferREACHNorwegiannorwegianreach
eVHB BufferREACHSpanishspanishreach
eVHB BufferREACHSwedishswedishreach
eVHB BufferWHMSEnglishenglishwhms
eVHB BufferWHMSFrenchfrenchwhms
iHDQ Binding BufferGHSEnglishenglishghs
iHDQ Binding BufferGHSSpanishspanishghs
iHDQ Binding BufferREACHEnglishenglishreach
iHDQ Binding BufferREACHDanishdanishreach
iHDQ Binding BufferREACHFinnishfinnishreach
iHDQ Binding BufferREACHFrenchfrenchreach
iHDQ Binding BufferREACHGermangermanreach
iHDQ Binding BufferREACHItalianitalianreach
iHDQ Binding BufferREACHNorwegiannorwegianreach
iHDQ Binding BufferREACHSpanishspanishreach
iHDQ Binding BufferREACHSwedishswedishreach
iHDQ Binding BufferWHMSEnglishenglishwhms
iHDQ Binding BufferWHMSFrenchfrenchwhms
Mag-Bind Particles PF-HDQGHSEnglishenglishghs
Mag-Bind Particles PF-HDQGHSSpanishspanishghs
Mag-Bind Particles PF-HDQREACHEnglishenglishreach
Mag-Bind Particles PF-HDQREACHDanishdanishreach
Mag-Bind Particles PF-HDQREACHFinnishfinnishreach
Mag-Bind Particles PF-HDQREACHFrenchfrenchreach
Mag-Bind Particles PF-HDQREACHGermangermanreach
Mag-Bind Particles PF-HDQREACHItalianitalianreach
Mag-Bind Particles PF-HDQREACHNorwegiannorwegianreach
Mag-Bind Particles PF-HDQREACHSpanishspanishreach
Mag-Bind Particles PF-HDQREACHSwedishswedishreach
Mag-Bind Particles PF-HDQWHMSEnglishenglishwhms
Mag-Bind Particles PF-HDQWHMSFrenchfrenchwhms
PCR Neutralization BufferREACHSpanishspanishreach
PCR Neutralization BufferREACHSwedishswedishreach
PCR Neutralization BufferWHMSEnglishenglishwhms
PCR Neutralization BufferWHMSFrenchfrenchwhms
Proteinase K SolutionGHSEnglishenglishghs
Proteinase K SolutionGHSSpanishspanishghs
Proteinase K SolutionREACHEnglishenglishreach
Proteinase K SolutionREACHDanishdanishreach
Proteinase K SolutionREACHFinnishfinnishreach
Proteinase K SolutionREACHFrenchfrenchreach
Proteinase K SolutionREACHGermangermanreach
Proteinase K SolutionREACHItalianitalianreach
Proteinase K SolutionREACHNorwegiannorwegianreach
Proteinase K SolutionREACHSpanishspanishreach
Proteinase K SolutionREACHSwedishswedishreach
Proteinase K SolutionWHMSEnglishenglishwhms
Proteinase K SolutionWHMSFrenchfrenchwhms
Proteinase K SolutionREACHDutchdutchreach
Proteinase K SolutionREACHHungarianhungarianreach
Proteinase K SolutionREACHPortugueseportuguesereach
TL BufferGHSEnglishenglishghs
TL BufferGHSSpanishspanishghs
TL BufferREACHEnglishenglishreach
TL BufferREACHDanishdanishreach
TL BufferREACHFinnishfinnishreach
TL BufferREACHFrenchfrenchreach
TL BufferREACHGermangermanreach
TL BufferREACHItalianitalianreach
TL BufferREACHNorwegiannorwegianreach
TL BufferREACHSpanishspanishreach
TL BufferREACHSwedishswedishreach
TL BufferWHMSEnglishenglishwhms
TL BufferWHMSFrenchfrenchwhms
TL BufferREACHDutchdutchreach
TL BufferREACHHungarianhungarianreach
TL BufferREACHPortugueseportuguesereach
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