Mag-Bind® cfDNA Kit

$0.00$2,475.90

Rapid & efficient isolation of circulating, cell-free DNA from 0.5-10 mL plasma or serum samples

  • Isolate cell-free DNA (cfDNA) from up to 10 mL Plasma or Serum
  • Automation-ready scripts process 96 (1 mL) samples in 2 hours
  • High-binding magnetic beads allow 4 mL serum or plasma to be eluted in 50 µL

CE-IVD marked kit is available: Mag-Bind® cfDNA Kit CE IVD – (omegabiotek.com)

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

The Mag-Bind® cfDNA Kit is designed for the rapid and efficient isolation of circulating cell-free DNA from up to 10 mL plasma or serum samples. The Mag-Bind cfDNA Kit can be processed manually or using automated platforms. The procedure eliminates the need for funnels and vacuum steps, providing hands-free operation in automated protocols.

The unique formulation of the lysis and binding buffers allows complete automation of the extraction process for up to 10 mL sample volumes with minimal user intervention. The magnetic properties of the Mag-Bind Particles CH enable fast magnetic separation, even when using large volumes. The high binding capacity of the beads allows for a lower volume of magnetic particles needed, thus reducing the final elution volume required. 10 mL of serum or plasma can be eluted in as low as 50 μL.

The system combines the reversible nucleic acid-binding properties of Mag-Bind paramagnetic particles with a unique binding system that targets smaller DNA fragments (150-400 bp) and minimizes the binding of larger fragments, such as gDNA.

This Kit can be automated on most open liquid handlers or magnetic processors. Ready-to-implement protocols are available for the following platforms:

  • Dynamic Devices Lynx
  • Hamilton Microlab® STAR
  • Thermo Fisher Scientific KingFisher® Apex
  • Thermo Fisher Scientific KingFisher® Flex

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Sample TypePlasma/Serum
Sample Volume500-10,000 µL
TechnologyMagnetic Beads
ProcessingManual or Automated
AutomatableYes
Processing Time2 hours for 96 (1mL) samples
FormatTube, 24-well, 96-well
Elution Volume50 µL
ITEMAVAILABLE SEPARATELY
Mag-Bind® Particles CHCall for Pricing
DS BufferCall for Pricing
JSB BufferView Product
GT7 Buffer v1.1Call for Pricing
SPW BufferView Product
Elution BufferView Product
Proteinase K SolutionView Product

Little Genomic Carryover

Figure 1. Electropherogram overlay of purified DNA from 4 mL serum. 4 mL of unspiked serum was purified using kits from Omega Bio-tek and Company Q following manufacturer’s recommended protocols. Purified DNA was analyzed on Agilent’s TapeStation® 2200.

High Yield and Quality

Figure 2.  Electropherogram overlay of purified DNA from 1 mL serum. Sheared, bacterial DNA was spiked into serum samples and isolated using Omega Bio-tek’s Mag-Bind cfDNA Kit and a column-based kit from Company Q. Real-time PCR was performed using 16s bacteria-specific primers at 1X and 10X dilutions. Purified DNA was analyzed on Agilent’s TapeStation® 2200. The Omega Bio-tek kit was able to capture the circulating, cell-free DNA with no genomic DNA contamination. In contrast, Company Q’s eluate contained high molecular weight fragments indicating the presence of genomic DNA in the circulating DNA isolation.

No PCR Inhibitors

Table 1.  Real-time PCR with 16S bacterial-specific primers was performed on triplicates of undiluted and 10-fold dilutions of DNA isolated from samples purified [figure 2]. The data shows the purified DNA is free of PCR inhibitors and the specific cfDNA concentration is higher than Company Q.

Recover Fragments as Small as 50 bp

Figure 3. DNA fragments as small as 50 bp can be recovered using modified protocol. Serum spiked with 50 bp DNA ladder was purified with the Mag-Bind® cfDNA Kit. By using modified protocols, smaller fragments were recovered.

TapeStation Analysis

Figure 4. TapeStation® analysis of cfDNA purified from 10 mL of unspiked plasma samples (n = 12) using Omega Bio-tek’s kit automated on Hamilton Microlab® STAR™ platform.

Table 2. Gel image from figure 4.1 was analyzed using the TapeStation software. Peak and concentration data showed that 8 mL automation process was a success.

Figure 5. Gel image from figure 4.1 was analyzed using the TapeStation software. Peak and concentration data showed that 8 mL automation process was a success.

Product Information

NameDocumentLanguagesLink
Quick GuideProduct ManualEnglish
Protocol ManualProduct ManualEnglish
Mag-Bind® cfDNA KitProduct LiteratureEnglish
Extraction & Quality Analysis of Circulating, Cell-Free DNA from Serum Samples Using Omega Bio-tek KitsApplication NoteEnglish
Strategies for retrieving cfDNA of short fragment lengths using Mag-Bind® cfDNA Kit from Omega Bio-tekApplication NoteEnglish
Automated cfDNA Purification from up to Ninety-six, 4 mL Plasma Samples Using the Mag-Bind® NAP STARApplication NoteEnglish
Using ddPCR to Detect Rare Variant Alleles in cfDNA Samples on the MagBinder Fit24 PlatformApplication NoteEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DS BufferGHSEnglishenglishghs
DS BufferGHSSpanishspanishghs
DS BufferREACHEnglishenglishreach
DS BufferREACHDanishdanishreach
DS BufferREACHFinnishfinnishreach
DS BufferREACHFrenchfrenchreach
DS BufferREACHGermangermanreach
DS BufferREACHItalianitalianreach
DS BufferREACHNorwegiannorwegianreach
DS BufferREACHSpanishspanishreach
DS BufferREACHSwedishswedishreach
DS BufferWHMSEnglishenglishwhms
DS BufferWHMSFrenchfrenchwhms
DS BufferREACHDutchdutchreach
DS BufferREACHHungarianhungarianreach
DS BufferREACHPortugueseportuguesereach
DS BufferREACHGreekgreekreach
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHGreekgreekreach
GT7 Buffer v1.1REACHDanishdanishreach
GT7 Buffer v1.1REACHDutchdutchreach
GT7 Buffer v1.1REACHEnglishenglishreach
GT7 Buffer v1.1REACHFrenchfrenchreach
GT7 Buffer v1.1REACHGermangermanreach
GT7 Buffer v1.1REACHHungarianhungarianreach
GT7 Buffer v1.1REACHItalianitalianreach
GT7 Buffer v1.1REACHNorwegiannorwegianreach
GT7 Buffer v1.1REACHPortugueseportuguesereach
GT7 Buffer v1.1REACHSpanishspanishreach
GT7 Buffer v1.1REACHSwedishswedishreach
GT7 Buffer v1.1GHSEnglishenglishghs
GT7 Buffer v1.1GHSSpanishspanishghs
GT7 Buffer v1.1REACHFinnishfinnishreach
GT7 Buffer v1.1WHMSEnglishenglishwhms
GT7 Buffer v1.1WHMSFrenchfrenchwhms
GT7 Buffer v1.1REACHGreekgreekreach
JSB BufferGHSEnglishenglishghs
JSB BufferGHSSpanishspanishghs
JSB BufferREACHEnglishenglishreach
JSB BufferREACHDanishdanishreach
JSB BufferREACHFinnishfinnishreach
JSB BufferREACHFrenchfrenchreach
JSB BufferREACHGermangermanreach
JSB BufferREACHItalianitalianreach
JSB BufferREACHNorwegiannorwegianreach
JSB BufferREACHSpanishspanishreach
JSB BufferREACHSwedishswedishreach
JSB BufferWHMSEnglishenglishwhms
JSB BufferWHMSFrenchfrenchwhms
JSB BufferREACHDutchdutchreach
JSB BufferREACHHungarianhungarianreach
JSB BufferREACHPortugueseportuguesereach
JSB BufferREACHGreekgreekreach
Mag-Bind Particles CHGHSEnglishenglishghs
Mag-Bind Particles CHGHSSpanishspanishghs
Mag-Bind Particles CHREACHEnglishenglishreach
Mag-Bind Particles CHREACHDanishdanishreach
Mag-Bind Particles CHREACHFinnishfinnishreach
Mag-Bind Particles CHREACHFrenchfrenchreach
Mag-Bind Particles CHREACHGermangermanreach
Mag-Bind Particles CHREACHItalianitalianreach
Mag-Bind Particles CHREACHNorwegiannorwegianreach
Mag-Bind Particles CHREACHSpanishspanishreach
Mag-Bind Particles CHREACHSwedishswedishreach
Mag-Bind Particles CHWHMSEnglishenglishwhms
Mag-Bind Particles CHWHMSFrenchfrenchwhms
Mag-Bind Particles CHREACHDutchdutchreach
Mag-Bind Particles CHREACHHungarianhungarianreach
Mag-Bind Particles CHREACHPortugueseportuguesereach
Mag-Bind Particles CHREACHGreekgreekreach
Proteinase K SolutionGHSEnglishenglishghs
Proteinase K SolutionGHSSpanishspanishghs
Proteinase K SolutionREACHEnglishenglishreach
Proteinase K SolutionREACHDanishdanishreach
Proteinase K SolutionREACHFinnishfinnishreach
Proteinase K SolutionREACHFrenchfrenchreach
Proteinase K SolutionREACHGermangermanreach
Proteinase K SolutionREACHItalianitalianreach
Proteinase K SolutionREACHNorwegiannorwegianreach
Proteinase K SolutionREACHSpanishspanishreach
Proteinase K SolutionREACHSwedishswedishreach
Proteinase K SolutionWHMSEnglishenglishwhms
Proteinase K SolutionWHMSFrenchfrenchwhms
Proteinase K SolutionREACHDutchdutchreach
Proteinase K SolutionREACHHungarianhungarianreach
Proteinase K SolutionREACHPortugueseportuguesereach
Proteinase K SolutionREACHGreekgreekreach
SPW BufferREACHDanishdanishreach
SPW BufferREACHDutchdutchreach
SPW BufferREACHEnglishenglishreach
SPW BufferREACHFrenchfrenchreach
SPW BufferREACHGermangermanreach
SPW BufferREACHHungarianhungarianreach
SPW BufferREACHItalianitalianreach
SPW BufferREACHNorwegiannorwegianreach
SPW BufferREACHPortugueseportuguesereach
SPW BufferREACHSpanishspanishreach
SPW BufferREACHSwedishswedishreach
SPW BufferGHSEnglishenglishghs
SPW BufferGHSSpanishspanishghs
SPW BufferWHMSEnglishenglishwhms
SPW BufferWHMSFrenchfrenchwhms
SPW BufferREACHGreekgreekreach
SPW BufferREACHFinnishfinnishreach
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