E.Z.N.A.® Soil DNA Kit


Rapid and reliable isolation of DNA from soil samples

  • Reliable – Reproducible DNA purification from a variety of sample sources
  • High quality – Ready-to-use DNA eliminating PCR inhibitors using proprietary inhibitor removal technology
  • Yield – Efficient purification of DNA from even specialized samples
  • Ease of use – Contains glass beads pre-filled in 2 mL vials

The E.Z.N.A.® Soil DNA Kit allows rapid and reliable isolation of high-quality genomic DNA from various soil samples. This kit can isolate microbial DNA from yeast, fungi, and grampositive or negative bacteria that inhabit a range of samples including clay, sandy, peaty, chalky, or loamy soil samples. This kit not only includes Disruptor Tubes prefilled with glass beads for efficient sample homogenization but also features a unique inhibitor removal reagent (cHTR Reagent) for effective removal of humic acid and other PCR inhibitors from eluted DNA. The extraction methodology combines the reversible nucleic acid-binding properties of HiBind® matrix with the speed and versatility of spin column technology for DNA purification. Up to 250 mg soil samples can be processed in 60 minutes or up to 1g soil samples in 2.5 hours. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.

For Research Use Only. Not for use in diagnostic procedures.

Starting AmountUp to 1 g
Starting MaterialSoil
YieldDependent upon sample
Elution Volume50-100 μL
TechnologyHiBind® DNA Mini Column
Processing ModeManual
HiBind® DNA Mini ColumnsView Product
2 mL Collection TubesView Product
Disruptor TubesView Product
SLX-Mlus BufferView Product
DS BufferCall for Pricing
P2 BufferView Product
cHTR Reagent
XP1 Buffer
HBC BufferView Product
DNA Wash BufferView Product
Elution BufferView Product

Yield Comparison

Figure 1. Comparison of DNA extraction method from soil samples. DNA yield determined with fluorescence-based dye quantification. 50 µL ZymoBIOMICS™ Microbial Community Standard was added to 200 mg soil samples and DNA was extracted using manufacturer’s recommended protocols. DNA was eluted in 100 µL for both manufacturers.

qPCR Comparison

Figure 2. Comparison of Ct values. 20 µL SYBR Green qPCR reaction. 50 µL ZymoBIOMICS™ Microbial Community Standard was added to 200 mg soil samples and DNA was extracted using manufacturer’s recommended protocols. DNA was eluted in 100 µL for both manufacturers.

Bacteria Yield Comparison

Figure 3. DNA yield by bacterial classes. DNA yield determined with fluorescence-based dye quantification. 0.5 mL cultured Gram-positive and Gram-negative bacteria were added to corresponding 200 mg soil samples and DNA was extracted using manufacturer’s recommended protocols. DNA was eluted in 100 µL for both manufacturers.

I left my cHTR reagent out at room temperature for a few days. Is it ok to use?

We have stability data showing that the reagent is fine for three months at room temperature with no effect on quality. So it is ok to use. Please store at recommended temperature going forward.

I noticed precipitates in the cHTR buffer- is that normal? It is also difficult to pipette.

Precipitates are normal in cHTR. Please shake well before using and cut the pipette tip to help with aspiration.

Product Information

Quick GuideProduct ManualEnglish
Protocol ManualProduct ManualEnglish
E.Z.N.A.® Soil DNA KitProduct LiteratureEnglish
Superior Performance of Omega Bio-tek’s E.Z.N.A.® Soil DNA KitApplication NoteEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
cHTR ReagentGHSEnglishenglishghs
cHTR ReagentGHSSpanishspanishghs
cHTR ReagentREACHEnglishenglishreach
cHTR ReagentREACHDanishdanishreach
cHTR ReagentREACHFinnishfinnishreach
cHTR ReagentREACHFrenchfrenchreach
cHTR ReagentREACHGermangermanreach
cHTR ReagentREACHItalianitalianreach
cHTR ReagentREACHNorwegiannorwegianreach
cHTR ReagentREACHSpanishspanishreach
cHTR ReagentREACHSwedishswedishreach
cHTR ReagentWHMSEnglishenglishwhms
cHTR ReagentWHMSFrenchfrenchwhms
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferREACHEnglishenglishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferWHMSFrenchfrenchwhms
DS BufferGHSEnglishenglishghs
DS BufferGHSSpanishspanishghs
DS BufferREACHEnglishenglishreach
DS BufferREACHDanishdanishreach
DS BufferREACHFinnishfinnishreach
DS BufferREACHFrenchfrenchreach
DS BufferREACHGermangermanreach
DS BufferREACHItalianitalianreach
DS BufferREACHNorwegiannorwegianreach
DS BufferREACHSpanishspanishreach
DS BufferREACHSwedishswedishreach
DS BufferWHMSEnglishenglishwhms
DS BufferWHMSFrenchfrenchwhms
DS BufferREACHDutchdutchreach
DS BufferREACHHungarianhungarianreach
DS BufferREACHPortugueseportuguesereach
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
HBC BufferGHSEnglishenglishghs
HBC BufferGHSSpanishspanishghs
HBC BufferREACHEnglishenglishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHFinnishfinnishreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHGermangermanreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHSpanishspanishreach
HBC BufferREACHSwedishswedishreach
HBC BufferWHMSEnglishenglishwhms
HBC BufferWHMSFrenchfrenchwhms
P2 BufferGHSEnglishenglishghs
P2 BufferGHSSpanishspanishghs
P2 BufferREACHEnglishenglishreach
P2 BufferREACHDanishdanishreach
P2 BufferREACHFinnishfinnishreach
P2 BufferREACHFrenchfrenchreach
P2 BufferREACHGermangermanreach
P2 BufferREACHItalianitalianreach
P2 BufferREACHNorwegiannorwegianreach
P2 BufferREACHSpanishspanishreach
P2 BufferREACHSwedishswedishreach
P2 BufferWHMSEnglishenglishwhms
P2 BufferWHMSFrenchfrenchwhms
SLX M1us BufferGHSEnglishenglishghs
SLX M1us BufferGHSSpanishspanishghs
SLX M1us BufferREACHEnglishenglishreach
SLX M1us BufferREACHDanishdanishreach
SLX M1us BufferREACHFinnishfinnishreach
SLX M1us BufferREACHFrenchfrenchreach
SLX M1us BufferREACHGermangermanreach
SLX M1us BufferREACHItalianitalianreach
SLX M1us BufferREACHNorwegiannorwegianreach
SLX M1us BufferREACHSpanishspanishreach
SLX M1us BufferREACHSwedishswedishreach
SLX M1us BufferWHMSEnglishenglishwhms
SLX M1us BufferWHMSFrenchfrenchwhms
XP1 BufferGHSEnglishenglishghs
XP1 BufferGHSSpanishspanishghs
XP1 BufferREACHEnglishenglishreach
XP1 BufferREACHDanishdanishreach
XP1 BufferREACHFinnishfinnishreach
XP1 BufferREACHFrenchfrenchreach
XP1 BufferREACHGermangermanreach
XP1 BufferREACHItalianitalianreach
XP1 BufferREACHNorwegiannorwegianreach
XP1 BufferREACHSpanishspanishreach
XP1 BufferREACHSwedishswedishreach
XP1 BufferWHMSEnglishenglishwhms
XP1 BufferWHMSFrenchfrenchwhms
DS BufferREACHGreekgreekreach
Elution BufferREACHGreekgreekreach
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