Mag-Bind® Total RNA 96 Kit

Can I isolate miRNA along with Total RNA using M6731?

Yes, you can as long as you follow some protocol modifications. At step 5 on page 6, add 400 µL of 100% ethanol instead of 300 µL. At step 26 on page 8, add 480 µL of RNA Wash Buffer II instead of 300 µL.

Can samples be stored in OTRK Lysis Buffer prior to extraction?

Homogenized cell/tissue lysates in OTRK Lysis Buffer can be stored at -80 °C for at least 6 months. To proceed with the frozen lysates, thaw the sample at 37 °C until they are completely thawed, and all salts in the lysis buffer are dissolved. Do not extend the treatment in 37 °C because it can cause chemical degradation of RNA.

Can I use tissues in RNAlater™ with M6731?

The RNAlater will interfere with our buffer chemistry resulting in low yields so tissues must be washed before starting the extraction.

1. Spin down the tissue at 500 x g for 5 minutes.
2. Aspirate out the supernatant.
3. Resuspend the pellet with 1% PBS Buffer.
4. Spin down the sample again (500 x g for 5 minutes), then aspirate out the supernatant.
5. Use the pelleted tissue for extraction following the Mag-Bind Total RNA 96 Kit Protocol- Tissue on page 5.