$0.00 – $1,226.10
Isolate total cellular RNA and viral RNA from a wide variety of cells and tissues
All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.
Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.
Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.
Mag-Bind® Total RNA Kit provides a novel technology for high-quality total RNA isolation from a wide variety of cells and tissues. Total RNA can be purified from 5-10 mg of tissue or 1×106 cultured cells. The system combines the efficient reversible nucleic acid- binding properties of Mag-Bind® particles with our RNA buffer chemistry to provide superior quality RNA. Unlike column- based systems, the binding of nucleic acids to magnetic particles occurs in solution, resulting in increased binding kinetics and binding efficiency. Particles are also completely re-suspended during the wash steps or the purification protocol, which improves removal of contaminants and increases nucleic acid purity. The Mag-Bind® Total RNA 96 procedure can be fully automated on most robotic workstations.
Protocols are available for the following automated platforms:
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Application | PCR, Next Generation Sequencing, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification |
| Elution Volume | 30-50 µL |
| Starting Material | Animal Tissue and Cultured Cells |
| Starting Amount | 1 x 106 cells or 10 mg tissue |
| Processing Mode | Automated; Manual |
| Throughput | Up to 96 |
| RNA Binding Technology | Magnetic Beads |
Figure 1. RNA Integrity. Total RNA was extracted from 1×106 cells using the Mag-Bind® Total RNA Kit. The RNA samples were analyzed with RNA tape on Agilent’s TapeStation® 2200.
Figure 2. RNA Yield Comparison. RNA was isolated using the Mag-Bind® Total RNA Kit and a comparable column-based kit from Company Q. Elution volumes for company Q were 50 µL; for Omega were 100 µL, RNA was quantified using Thermo Scientific’s NanoDrop® 2000c
Figure 3. 10 mg mouse liver was isolated with the Mag-Bind Total RNA Kit according to protocol. Total RNA concentration determined by optical density measurements using Thermo Scientific’s NanoDrop® 2000c. Total elution volume was 100 µL
Yes, you can as long as you follow some protocol modifications. At step 5 on page 6, add 400 µL of 100% ethanol instead of 300 µL. At step 26 on page 8, add 480 µL of RNA Wash Buffer II instead of 300 µL.
Homogenized cell/tissue lysates in OTRK Lysis Buffer can be stored at -80 °C for at least 6 months. To proceed with the frozen lysates, thaw the sample at 37 °C until they are completely thawed, and all salts in the lysis buffer are dissolved. Do not extend the treatment in 37 °C because it can cause chemical degradation of RNA.
The RNAlater will interfere with our buffer chemistry resulting in low yields so tissues must be washed before starting the extraction.
