Mag-Bind® Total RNA 96 Kit


Isolate total cellular RNA and viral RNA from a wide variety of cells and tissues

  • Process 96 samples in 90 minutes
  • Bead-based scalable RNA purification
  • High-quality RNA suitable for demanding downstream applications
  • Adaptable on most open-ended liquid handlers

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

Mag-Bind® Total RNA Kit provides a novel technology for high-quality total RNA isolation from a wide variety of cells and tissues. Total RNA can be purified from 5-10 mg of tissue or 1×106 cultured cells. The system combines the efficient reversible nucleic acid- binding properties of Mag-Bind® particles with our RNA buffer chemistry to provide superior quality RNA. Unlike column- based systems, the binding of nucleic acids to magnetic particles occurs in solution, resulting in increased binding kinetics and binding efficiency. Particles are also completely re-suspended during the wash steps or the purification protocol, which improves removal of contaminants and increases nucleic acid purity. The Mag-Bind® Total RNA 96 procedure can be fully automated on most robotic workstations.

Protocols are available for the following automated platforms:

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationPCR, Next Generation Sequencing, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume30-50 µL
Starting MaterialAnimal Tissue and Cultured Cells
Starting Amount1 x 106 cells or 10 mg tissue
Processing ModeAutomated; Manual
ThroughputUp to 96
RNA Binding TechnologyMagnetic Beads

High Quality RNA

Figure 1. RNA Integrity. Total RNA was extracted from 1×106 cells using the Mag-Bind® Total RNA Kit. The RNA samples were analyzed with RNA tape on Agilent’s TapeStation® 2200.

Yield Comparison

Figure 2. RNA Yield Comparison. RNA was isolated using the Mag-Bind® Total RNA Kit and a comparable column-based kit from Company Q. Elution volumes for company Q were 50 µL; for Omega were 100 µL,  RNA was quantified using Thermo Scientific’s NanoDrop® 2000c

RNA Yield from Mouse Liver

Figure 3. 10 mg mouse liver was isolated with the Mag-Bind Total RNA Kit according to protocol. Total RNA concentration determined by optical density measurements using Thermo Scientific’s NanoDrop® 2000c. Total elution volume was 100 µL

Can I isolate miRNA along with Total RNA using M6731?

Yes, you can as long as you follow some protocol modifications. At step 5 on page 6, add 400 µL of 100% ethanol instead of 300 µL. At step 26 on page 8, add 480 µL of RNA Wash Buffer II instead of 300 µL.

Can samples be stored in OTRK Lysis Buffer prior to extraction?

Homogenized cell/tissue lysates in OTRK Lysis Buffer can be stored at -80 °C for at least 6 months. To proceed with the frozen lysates, thaw the sample at 37 °C until they are completely thawed, and all salts in the lysis buffer are dissolved. Do not extend the treatment in 37 °C because it can cause chemical degradation of RNA.

Can I use tissues in RNAlater™ with M6731?

The RNAlater will interfere with our buffer chemistry resulting in low yields so tissues must be washed before starting the extraction.

  1. Spin down the tissue at 500 x g for 5 minutes.
  2. Aspirate out the supernatant.
  3. Resuspend the pellet with 1% PBS Buffer.
  4. Spin down the sample again (500 x g for 5 minutes), then aspirate out the supernatant.
  5. Use the pelleted tissue for extraction following the Mag-Bind Total RNA 96 Kit Protocol- Tissue on page 5.

Product Information

Document NameLanguagesLink
Protocol ManualEnglish
Mag-Bind® Total RNA 96 KitEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DNase I Digestion BufferGHSEnglishenglishghs
DNase I Digestion BufferGHSSpanishspanishghs
DNase I Digestion BufferREACHEnglishenglishreach
DNase I Digestion BufferREACHDanishdanishreach
DNase I Digestion BufferREACHFinnishfinnishreach
DNase I Digestion BufferREACHFrenchfrenchreach
DNase I Digestion BufferREACHGermangermanreach
DNase I Digestion BufferREACHItalianitalianreach
DNase I Digestion BufferREACHNorwegiannorwegianreach
DNase I Digestion BufferREACHSpanishspanishreach
DNase I Digestion BufferREACHSwedishswedishreach
DNase I Digestion BufferWHMSEnglishenglishwhms
DNase I Digestion BufferWHMSFrenchfrenchwhms
Mag-Bind DNase IGHSEnglishenglishghs
Mag-Bind DNase IGHSSpanishspanishghs
Mag-Bind DNase IREACHEnglishenglishreach
Mag-Bind DNase IREACHDanishdanishreach
Mag-Bind DNase IREACHFinnishfinnishreach
Mag-Bind DNase IREACHFrenchfrenchreach
Mag-Bind DNase IREACHGermangermanreach
Mag-Bind DNase IREACHItalianitalianreach
Mag-Bind DNase IREACHNorwegiannorwegianreach
Mag-Bind DNase IREACHSpanishspanishreach
Mag-Bind DNase IREACHSwedishswedishreach
Mag-Bind DNase IWHMSEnglishenglishwhms
Mag-Bind DNase IWHMSFrenchfrenchwhms
Mag-Bind Particles CNRGHSEnglishenglishghs
Mag-Bind Particles CNRGHSSpanishspanishghs
Mag-Bind Particles CNRREACHEnglishenglishreach
Mag-Bind Particles CNRREACHDanishdanishreach
Mag-Bind Particles CNRREACHFinnishfinnishreach
Mag-Bind Particles CNRREACHFrenchfrenchreach
Mag-Bind Particles CNRREACHGermangermanreach
Mag-Bind Particles CNRREACHItalianitalianreach
Mag-Bind Particles CNRREACHNorwegiannorwegianreach
Mag-Bind Particles CNRREACHSpanishspanishreach
Mag-Bind Particles CNRREACHSwedishswedishreach
Mag-Bind Particles CNRWHMSEnglishenglishwhms
Mag-Bind Particles CNRWHMSFrenchfrenchwhms
OTRK BufferGHSEnglishenglishghs
OTRK BufferGHSSpanishspanishghs
OTRK BufferREACHEnglishenglishreach
OTRK BufferREACHDanishdanishreach
OTRK BufferREACHFinnishfinnishreach
OTRK BufferREACHFrenchfrenchreach
OTRK BufferREACHGermangermanreach
OTRK BufferREACHItalianitalianreach
OTRK BufferREACHNorwegiannorwegianreach
OTRK BufferREACHSpanishspanishreach
OTRK BufferREACHSwedishswedishreach
OTRK BufferWHMSEnglishenglishwhms
OTRK BufferWHMSFrenchfrenchwhms
PHM BufferGHSEnglishenglishghs
PHM BufferGHSSpanishspanishghs
PHM BufferREACHEnglishenglishreach
PHM BufferREACHDanishdanishreach
PHM BufferREACHFinnishfinnishreach
PHM BufferREACHFrenchfrenchreach
PHM BufferREACHGermangermanreach
PHM BufferREACHItalianitalianreach
PHM BufferREACHNorwegiannorwegianreach
PHM BufferREACHSpanishspanishreach
PHM BufferREACHSwedishswedishreach
PHM BufferWHMSEnglishenglishwhms
PHM BufferWHMSFrenchfrenchwhms
Proteinase K SolutionGHSEnglishenglishghs
Proteinase K SolutionGHSSpanishspanishghs
Proteinase K SolutionREACHEnglishenglishreach
Proteinase K SolutionREACHDanishdanishreach
Proteinase K SolutionREACHFinnishfinnishreach
Proteinase K SolutionREACHFrenchfrenchreach
Proteinase K SolutionREACHGermangermanreach
Proteinase K SolutionREACHItalianitalianreach
Proteinase K SolutionREACHNorwegiannorwegianreach
Proteinase K SolutionREACHSpanishspanishreach
Proteinase K SolutionREACHSwedishswedishreach
Proteinase K SolutionWHMSEnglishenglishwhms
Proteinase K SolutionWHMSFrenchfrenchwhms
Proteinase K SolutionREACHDutchdutchreach
Proteinase K SolutionREACHHungarianhungarianreach
Proteinase K SolutionREACHPortugueseportuguesereach
RNA Elution BufferGHSEnglishenglishghs
RNA Elution BufferGHSSpanishspanishghs
RNA Elution BufferREACHEnglishenglishreach
RNA Elution BufferREACHDanishdanishreach
RNA Elution BufferREACHFinnishfinnishreach
RNA Elution BufferREACHFrenchfrenchreach
RNA Elution BufferREACHGermangermanreach
RNA Elution BufferREACHItalianitalianreach
RNA Elution BufferREACHNorwegiannorwegianreach
RNA Elution BufferREACHSpanishspanishreach
RNA Elution BufferREACHSwedishswedishreach
RNA Elution BufferWHMSEnglishenglishwhms
RNA Elution BufferWHMSFrenchfrenchwhms
RNA Wash Buffer IIGHSEnglishenglishghs
RNA Wash Buffer IIGHSSpanishspanishghs
RNA Wash Buffer IIREACHEnglishenglishreach
RNA Wash Buffer IIREACHDanishdanishreach
RNA Wash Buffer IIREACHFinnishfinnishreach
RNA Wash Buffer IIREACHFrenchfrenchreach
RNA Wash Buffer IIREACHGermangermanreach
RNA Wash Buffer IIREACHItalianitalianreach
RNA Wash Buffer IIREACHNorwegiannorwegianreach
RNA Wash Buffer IIREACHSpanishspanishreach
RNA Wash Buffer IIREACHSwedishswedishreach
RNA Wash Buffer IIWHMSEnglishenglishwhms
RNA Wash Buffer IIWHMSFrenchfrenchwhms
VHB BufferGHSEnglishenglishghs
VHB BufferGHSSpanishspanishghs
VHB BufferREACHEnglishenglishreach
VHB BufferREACHDanishdanishreach
VHB BufferREACHFinnishfinnishreach
VHB BufferREACHFrenchfrenchreach
VHB BufferREACHGermangermanreach
VHB BufferREACHItalianitalianreach
VHB BufferREACHNorwegiannorwegianreach
VHB BufferREACHSpanishspanishreach
VHB BufferREACHSwedishswedishreach
VHB BufferWHMSEnglishenglishwhms
VHB BufferWHMSFrenchfrenchwhms
VHB BufferREACHDutchdutchreach
VHB BufferREACHHungarianhungarianreach
VHB BufferREACHPortugueseportuguesereach
Proteinase K SolutionREACHGreekgreekreach
VHB BufferREACHGreekgreekreach
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