E.Z.N.A.® Viral RNA Kit


Isolate viral RNA from cell-free fluids such as plasma, serum, urine, and cell culture supernatant using spin columns.

• Viral RNA/DNA purification from wide variety of sample types
• Can be used to clean-up samples in Trizol
• No organic extractions

The E.Z.N.A. Viral RNA Kit is designed to isolate viral RNA and DNA from plasma, serum, cell culture supernatant and urine, swabs, and saliva. The procedure completely removes contaminants and enzyme inhibitors, making viral RNA isolation fast, convenient, and reliable. The purified nucleic acids are ready for direct use in downstream applications such as qPCR, RT-qPCR and more.

For Research Use Only. Not for use in diagnostic procedures.

Starting Amount150 µL
Starting MaterialCell free fluids such as plasma, serum, urine and cell culture supernatant
Elution Volume20-50 μL
TechnologyHiBind® RNA Mini Column
Processing ModeManual, Centrifugation/Vacuum
HiBind® RNA Mini Columns
2 mL Collection TubesView Product
QVL Lysis BufferView Product
RNA Wash Buffer IIView Product
VHB BufferView Product
Carrier RNA (Poly A)
Nuclease-Free waterView Product

Figure 1. 25 µL of Zeptomatrix Influenza A/B Positive Control was spiked into 125 µL serum samples and then Viral RNA was extracted using the E.Z.N.A.® Viral RNA Kit . 2 µL of Eluted RNA was used as a template in a 20 µL SYBR® qPCR reaction. C: inhibitor-free control; T: RNA sample extracted with kit. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template RNA in free of inhibitors.

Can the E.Z.N.A. Viral RNA Kit isolate DNA viruses as well?

Yes, this kit can be used to isolate DNA and RNA from viruses in cell-free fluids.

How do I reconstitute the Carrier RNA provided in the E.Z.N.A. Viral RNA Kit?

The instructions for reconstituting the Carrier RNA are provided in the “Preparing Reagents” section of the manual on Page 5.

For a 5-prep kit, add 50 µL of Nuclease-Free Water to the tube of lyophilized Carrier RNA, shake to dissolve completely, aliquot, and store at -20 °C.

For a 50-prep kit, add 320 µL of Nuclease-Free Water to the tube of lyophilized Carrier RNA, shake to dissolve completely, aliquot, and store at -20 °C.

Do not freeze/ thaw the aliquots more than 3 times.

Product Information

Document NameLanguagesLink
Quick GuideEnglish
Protocol ManualEnglish
Supplementary Protocol for NP Swabs, Aspirates and BAL SamplesEnglish
E.Z.N.A.® Viral RNA KitEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
Carrier RNAGHSSpanishspanishghs
Carrier RNAGHSEnglishenglishghs
Carrier RNAREACHFinnishfinnishreach
Carrier RNAREACHEnglishenglishreach
Carrier RNAREACHDanishdanishreach
Carrier RNAWHMSEnglishenglishwhms
Carrier RNAWHMSFrenchfrenchwhms
Carrier RNAREACHSwedishswedishreach
Carrier RNAREACHSpanishspanishreach
Carrier RNAREACHNorwegiannorwegianreach
Carrier RNAREACHItalianitalianreach
Carrier RNAREACHGermangermanreach
Carrier RNAREACHFrenchfrenchreach
Carrier RNAREACHDutchdutchreach
Carrier RNAREACHHungarianhungarianreach
Carrier RNAREACHPortugueseportuguesereach
Nuclease-free WaterGHSEnglishenglishghs
Nuclease-free WaterREACHFrenchfrenchreach
Nuclease-free WaterREACHGermangermanreach
Nuclease-free WaterREACHFinnishfinnishreach
Nuclease-free WaterREACHEnglishenglishreach
Nuclease-free WaterGHSSpanishspanishghs
Nuclease-free WaterREACHDanishdanishreach
Nuclease-free WaterREACHItalianitalianreach
Nuclease-free WaterREACHNorwegiannorwegianreach
Nuclease-free WaterREACHSpanishspanishreach
Nuclease-free WaterREACHSwedishswedishreach
Nuclease-free WaterREACHDutchdutchreach
Nuclease-free WaterREACHPortugueseportuguesereach
Nuclease-free WaterREACHHungarianhungarianreach
Nuclease-free WaterWHMSFrenchfrenchwhms
Nuclease-free WaterWHMSEnglishenglishwhms
QVL Lysis BufferGHSEnglishenglishghs
QVL Lysis BufferGHSSpanishspanishghs
QVL Lysis BufferREACHSwedishswedishreach
QVL Lysis BufferREACHSpanishspanishreach
QVL Lysis BufferREACHNorwegiannorwegianreach
QVL Lysis BufferREACHItalianitalianreach
QVL Lysis BufferREACHGermangermanreach
QVL Lysis BufferREACHFinnishfinnishreach
QVL Lysis BufferREACHDanishdanishreach
QVL Lysis BufferREACHEnglishenglishreach
QVL Lysis BufferREACHFrenchfrenchreach
QVL Lysis BufferWHMSEnglishenglishwhms
QVL Lysis BufferWHMSFrenchfrenchwhms
RNA Wash Buffer IIGHSEnglishenglishghs
RNA Wash Buffer IIGHSSpanishspanishghs
RNA Wash Buffer IIREACHEnglishenglishreach
RNA Wash Buffer IIREACHDanishdanishreach
RNA Wash Buffer IIREACHFinnishfinnishreach
RNA Wash Buffer IIREACHGermangermanreach
RNA Wash Buffer IIREACHFrenchfrenchreach
RNA Wash Buffer IIREACHItalianitalianreach
RNA Wash Buffer IIREACHNorwegiannorwegianreach
RNA Wash Buffer IIREACHSpanishspanishreach
RNA Wash Buffer IIREACHSwedishswedishreach
RNA Wash Buffer IIWHMSEnglishenglishwhms
RNA Wash Buffer IIWHMSFrenchfrenchwhms
VHB BufferGHSEnglishenglishghs
VHB BufferREACHEnglishenglishreach
VHB BufferGHSSpanishspanishghs
VHB BufferREACHDanishdanishreach
VHB BufferREACHFinnishfinnishreach
VHB BufferREACHFrenchfrenchreach
VHB BufferREACHGermangermanreach
VHB BufferREACHSwedishswedishreach
VHB BufferREACHSpanishspanishreach
VHB BufferREACHNorwegiannorwegianreach
VHB BufferREACHItalianitalianreach
VHB BufferWHMSEnglishenglishwhms
VHB BufferWHMSFrenchfrenchwhms
VHB BufferREACHDutchdutchreach
VHB BufferREACHHungarianhungarianreach
VHB BufferREACHPortugueseportuguesereach
Carrier RNAREACHGreekgreekreach
Nuclease-free WaterREACHGreekgreekreach
VHB BufferREACHGreekgreekreach
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