$0.00 – $2,323.20
High-throughput DNA and RNA isolation from cultured cells & tissue using magnetic beads
All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.
Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.
Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.
The Mag-Bind® DNA/RNA Kit is designed for simultaneous purification of DNA and RNA up to 1 x 106 cells and up to 10 mg tissue samples into two separate eluates from the same sample. The Kit is also capable of separate purification of miRNA fraction using an optional protocol. Extraction of DNA and RNA allows for comprehensive molecular analysis from the same sample source since there is no need for sample splitting.
The protocol uses an innovative Lysis/Binding master mix that conveniently lyses the sample first and upon addition of Mag-Bind® Particles CH binds the DNA to the particles. The RNA-containing supernatant is saved, and an RNA binding step is completed in the presence of binding buffer and isopropanol to bind RNA (>200 nt) to the second set of Mag-Bind® Particles CH. This results in separation of DNA and RNA into two fractions. The RNA-fraction is further treated with DNase I enzyme to digest the residual DNA and rebound to the same set of Mag-Bind® Particles CH by adjusting the binding conditions. Mag-Bind® Particles CH bound to DNA and RNA are individually washed and nucleic acids are eluted in two different tubes/microplates for further analyses. The purified DNA and RNA is ready for use in a wide range of downstream applications such as NGS, PCR, qPCR, RT-qPCR, etc.
The Mag-Bind® DNA/RNA Kit utilizes magnetic bead-based technology and can be processed either manually or automated on most open-ended liquid handling platforms as well as magnetic processors. Please follow the instructions outlined in the manual for tissues stored in preservation reagents such as DNA/RNA ShieldTM and RNAlaterTM.
For Research Use Only. Not for use in diagnostic procedures.
| Feature | Specifications |
|---|---|
| Starting Amount | 1 x 106 cells and 5-10 mg tissue |
| Starting Material | Cells, Fresh/Frozen Tissue, Tissues stored in Zymo Research's DNA/RNA Shield™ or Thermo Fisher's RNAlater™. |
| Elution Volume | 50 - 100 µL for DNA, 100 µL for RNA |
| Technology | Magnetic beads |
| Processing Mode | Automated, Manual |
| Throughput | 96 |
| Downstream Applications | NGS, PCR, qPCR, RT-qPCR, etc. |
| Note | Optional protocol included in the manual for purification of miRNA fraction. |
| SKU No | M6932-00 | M6932-01 | Available Seperately |
|---|---|---|---|
| Purifications | 1 x 96 | 4 x 96 | |
| RNA-Lock Reagent | 50 mL | 150 mL | R0424 RNA-Lock Reagent |
| GTC Lysis Buffer | 40 mL | 150 mL | |
| DRT Buffer | 5 mL | 20 mL | |
| KWB Buffer | 3 x 30mL | 9 x 30mL | |
| PCL Buffer | 75 mL | 250 mL | |
| DNase I Digestion Buffer | 25 mL | 25 mL | |
| PHM Buffer | 20 mL | 65 mL | |
| Elution Buffer | 15 mL | 50 mL | Elution Buffer |
| Nuclease-free Water | 40 mL | 150 mL | Nuclease Free Water |
| Mag-Bind® DNase I | 220 µL | 900 µL | |
| Mag-Bind® Particles CH | 4.4 mL | 20 mL | Mag-Bind® Particles CH |
Figure 1. TapeStation® analysis of purified DNA (A) and RNA (B) from 10 mg of different tissue types and 1×106 AD293 cells using Omega Bio-tek’s Mag-Bind® DNA/RNA Kit and Company Q’s kit. Omega Bio-tek’s kit resulted in high molecular weight DNA as well as higher DIN score underscoring superior quality DNA compared to Company Q. RIN scores were comparable for both kits. (DIN=DNA Integrity Number, RIN=RNA Integrity Number)
Figure 2. DNA and RNA were extracted from 5 mg of 4 different tissue types (n = 3) and one million AD293 cells using Mag-Bind® DNA/RNA Kit and comparable kits from Company Q and Company Z. Yields were measured by UV spectrophotometry. DNA yield was comparable where as the RNA recovery was higher for all the samples tested compared to kits from Company Q and Company Z.
Figure 3. Average Ct values obtained on 10X and 100X dilutions of DNA (A) and RNA (B) purified from 5 mg tissue samples The Ct values differed by ~ 3.3 cycles per 10-fold dilution indicating inhibitor-free DNA and RNA extracted from all the samples extracted using Omega Bio-tek’s kit.
Figure 4. TapeSation analysis of RNA purified from 10 mg liver following the optional miRNA purification protocol mentioned in the product manual. TapeStation analysis confirms purification miRNA and large RNA following the miRNA purification protocol.
Figure 5. DNA and RNA was extracted from 10 mg murine liver tissue preserved in either RNA-Lock Reagent, DNA/RNA Shield or RNAlater. DNA and RNA was eluted in 100 µL. The results corroborate the compatibility of Mag-Bind DNA/RNA Kit with various tissue storage and preservation solutions on the market.
