E.Z.N.A.® Blood DNA Mini Kit
Price range: $389.20 through $1,063.20
Isolate DNA from 1-250 µL whole blood using spin columns.
The E.Z.N.A.® Tissue DNA Kit is the recommended alternative.
The 600 prep size SKU#D3392-03 will be discontinued in 2027.
The E.Z.N.A.® Blood DNA Kit provides rapid total DNA isolation from 1-250 µL of fresh and frozen anticoagulated whole blood. The E.Z.N.A.® Blood DNA Kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and body fluids. This kit allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. DNA purified with the E.Z.N.A.® Blood DNA method is ready for applications such as PCR, Southern blotting, or restriction enzyme digestion.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Starting Amount | 1-250 µL |
| Starting Material | Fresh and frozen anticoagulated whole blood, body fluid, buccal swab, dried blood, buffy coat |
| Yield | 100 µg |
| Elution Volume | 100-200 μL |
| Technology | HiBind® DNA Mini Column |
| Processing Mode | Manual, centrifugation/vacuum |
| Throughput | 1-24 |
Higher Yields of Higher-Quality DNA vs Competitor
Figure 1. DNA was extracted from 250 µL whole blood using Omega Bio-tek’s E.Z.N.A.® Blood DNA Mini Kit and eluted in 200 µL. DNA was also extracted from 200 µL whole blood using an equivalent kit from Company Q, following manufacturer’s protocol. DNA concentrations and absorbance ratios were determined via Thermo Scientific’s NanoDrop™ 2000c. DNA concentration is reported with respect to the input volume of whole blood.
High Integrity DNA Isolation
Figure 2. DNA was extracted from 250 µL whole blood using Omega Bio-tek’s E.Z.N.A.® Blood DNA Mini Kit and eluted in 200 µL. DNA was also extracted from 200 µL whole blood using an equivalent kit from Company Q, following manufacturer’s instructions. DNA integrity was anlayzed via Agilent’s TapeStation® 4150. DNA concentrations for Company Q were outside the recommended range for the TapeStation.
Inhibitor-free DNA Isolation
Figure 3. DNA was extracted from 250 µL whole blood using Omega Bio-tek’s E.Z.N.A.® Blood DNA Mini Kit and eluted in 200 µL. DNA was also extracted from 200 µL whole blood using an equivalent kit from Company Q, following manufacturer’s protocol. Average Ct values were acquired using Applied Biosystems’ QuantStudio™ 3 Real-Time PCR System.
Product Information
Safety Data Sheets
| Components | Hazard Standards | Languages | Link | hf:tax:dlp_document_language | hf:tax:dlp_document_hazard-standard |
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| BL Buffer | GHS | Spanish | spanish | ghs | |
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| DNA Wash Buffer | REACH | French | french | reach | |
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| DNA Wash Buffer | WHMS | English | english | whms | |
| DNA Wash Buffer | WHMS | French | french | whms | |
| Elution Buffer | GHS | Spanish | spanish | ghs | |
| Elution Buffer | REACH | Polish | polish | reach | |
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| HBC Buffer | WHMS | English | english | whms | |
| HBC Buffer | GHS | English | english | ghs | |
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| HBC Buffer | REACH | English | english | reach | |
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| HBC Buffer | WHMS | French | french | whms | |
| Proteinase K Solution | REACH | German | german | reach | |
| Proteinase K Solution | GHS | English | english | ghs | |
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| Proteinase K Solution | REACH | English | english | reach | |
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| Proteinase K Solution | WHMS | English | english | whms | |
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| Proteinase K Solution | WHMS | French | french | whms |