Mag-Bind® Blood DNA HV Kit

$0.00$1,980.70

Updated in 2024!

Isolate DNA from up to 4 mL whole blood using magnetic beads.

CE-IVD marked kit is available: Mag-Bind® Blood DNA HV Kit CE IVD – (omegabiotek.com)

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

The Mag-Bind® Blood DNA HV Kit is intended for isolation and purification of genomic DNA from up to 4 mL whole blood samples.
The Mag-Bind® Blood DNA HV Kit utilizes magnetic bead-based technology and can be processed either manually or automated on most open-ended liquid handling platforms as well as magnetic processors. The Mag-Bind® Blood DNA HV Kit can be used to process
up to 2 mL whole blood samples on Omega Bio-tek’s MagBinder® Fit24 Nucleic Acid Purification System when user-filled into MagBinder compatible cartridges.

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Starting AmountUp to 4 mL
Starting MaterialWhole blood
Elution Volume0.4 – 1 mL
TechnologyMagnetic beads
Processing ModeAutomated, Manual
Throughput24
Note

Recovered DNA exhibits good purity with A260/A280 = ~1.8 and A260/A230 > 1.8.

High-quality DNA with excellent purity ratios

SampleDNA Yield (µg)A260/A280A260/A230
NanoDropQuantiFluor System
1119.21121.871.93
2120.11081.862.12
3114.91141.861.98
Table 1. DNA was manually extracted from 4 mL whole blood samples and was eluted in 1 mL volume. Purified DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system and Promega’s QuantiFluor® dsDNA system. The concordant readings indicate the DNA purified is primarily dsDNA.

qPCR Analysis of Purified DNA

Figure 1. Average Ct values obtained using human-specific primers on 10X and 100X dilutions of purified DNA used as the template. The Ct values differed by ~ 3 cycles per 10-fold dilution indicating inhibitor-free DNA.

DNA Yield from 2 mL whole blood samples purified on MagBinder Fit24 vs Manual

Figure 2. Average DNA Yield from 2 mL sample volume from three different lots of blood extracted manually vs automated on MagBinder® Fit24 Nucleic Acid Purification System. The DNA yield was determined using Thermo Scientific’s NanoDrop 2000c and found to be comparable between the manual and automated DNA extractions.

High Molecular Weight DNA extracted from blood samples

Figure 3. TapeStation® Analysis of Purified DNA. TapeStation® analysis of genomic DNA purified from 2 mL of whole blood samples using Omega Bio-tek’s Mag-Bind® Blood DNA HV Kit processed manually and on MagBinder® Fit24 Nucleic Acid Purification System.

Product Information

NameDocumentLanguagesLink
Protocol ManualProduct ManualEnglish
Automated DNA Purification Solution from 2 mL Whole Blood Using Omega Bio-tek’s Reagents on the MagBinder® Fit24 PlatformApplication Note

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
AL BufferGHSEnglishenglishghs
AL BufferGHSSpanishspanishghs
AL BufferREACHEnglishenglishreach
AL BufferREACHDanishdanishreach
AL BufferREACHFinnishfinnishreach
AL BufferREACHFrenchfrenchreach
AL BufferREACHGermangermanreach
AL BufferREACHItalianitalianreach
AL BufferREACHNorwegiannorwegianreach
AL BufferREACHSpanishspanishreach
AL BufferREACHSwedishswedishreach
AL BufferWHMSEnglishenglishwhms
AL BufferWHMSFrenchfrenchwhms
AL BufferREACHDutchdutchreach
AL BufferREACHHungarianhungarianreach
AL BufferREACHPortugueseportuguesereach
AL BufferREACHGreekgreekreach
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFinnishfinnishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHGermangermanreach
Elution BufferREACHItalianitalianreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHSwedishswedishreach
Elution BufferWHMSEnglishenglishwhms
Elution BufferWHMSFrenchfrenchwhms
Elution BufferREACHDutchdutchreach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHGreekgreekreach
HDQ Binding BufferGHSEnglishenglishghs
HDQ Binding BufferGHSSpanishspanishghs
HDQ Binding BufferREACHEnglishenglishreach
HDQ Binding BufferREACHDanishdanishreach
HDQ Binding BufferREACHFinnishfinnishreach
HDQ Binding BufferREACHFrenchfrenchreach
HDQ Binding BufferREACHGermangermanreach
HDQ Binding BufferREACHItalianitalianreach
HDQ Binding BufferREACHNorwegiannorwegianreach
HDQ Binding BufferREACHSpanishspanishreach
HDQ Binding BufferREACHSwedishswedishreach
HDQ Binding BufferWHMSEnglishenglishwhms
HDQ Binding BufferWHMSFrenchfrenchwhms
HDQ Binding BufferREACHDutchdutchreach
HDQ Binding BufferREACHHungarianhungarianreach
HDQ Binding BufferREACHPortugueseportuguesereach
HDQ Binding BufferREACHGreekgreekreach
KWB BufferGHSEnglishenglishghs
KWB BufferGHSSpanishspanishghs
KWB BufferWHMSEnglishenglishwhms
KWB BufferWHMSFrenchfrenchwhms
KWB BufferREACHEnglishenglishreach
KWB BufferREACHDanishdanishreach
KWB BufferREACHDutchdutchreach
KWB BufferREACHFinnishfinnishreach
KWB BufferREACHFrenchfrenchreach
KWB BufferREACHGermangermanreach
KWB BufferREACHGreekgreekreach
KWB BufferREACHHungarianhungarianreach
KWB BufferREACHItalianitalianreach
KWB BufferREACHNorwegiannorwegianreach
KWB BufferREACHPortugueseportuguesereach
KWB BufferREACHSpanishspanishreach
KWB BufferREACHSwedishswedishreach
Mag-Bind Particles CHGHSEnglishenglishghs
Mag-Bind Particles CHGHSSpanishspanishghs
Mag-Bind Particles CHREACHEnglishenglishreach
Mag-Bind Particles CHREACHDanishdanishreach
Mag-Bind Particles CHREACHFinnishfinnishreach
Mag-Bind Particles CHREACHFrenchfrenchreach
Mag-Bind Particles CHREACHGermangermanreach
Mag-Bind Particles CHREACHItalianitalianreach
Mag-Bind Particles CHREACHNorwegiannorwegianreach
Mag-Bind Particles CHREACHSpanishspanishreach
Mag-Bind Particles CHREACHSwedishswedishreach
Mag-Bind Particles CHWHMSEnglishenglishwhms
Mag-Bind Particles CHWHMSFrenchfrenchwhms
Mag-Bind Particles CHREACHDutchdutchreach
Mag-Bind Particles CHREACHHungarianhungarianreach
Mag-Bind Particles CHREACHPortugueseportuguesereach
Mag-Bind Particles CHREACHGreekgreekreach
RMP BufferGHSEnglishenglishghs
RMP BufferGHSSpanishspanishghs
RMP BufferREACHEnglishenglishreach
RMP BufferREACHDanishdanishreach
RMP BufferREACHFinnishfinnishreach
RMP BufferREACHFrenchfrenchreach
RMP BufferREACHGermangermanreach
RMP BufferREACHItalianitalianreach
RMP BufferREACHNorwegiannorwegianreach
RMP BufferREACHSpanishspanishreach
RMP BufferREACHSwedishswedishreach
RMP BufferWHMSEnglishenglishwhms
RMP BufferWHMSFrenchfrenchwhms
RMP BufferREACHDutchdutchreach
RMP BufferREACHHungarianhungarianreach
RMP BufferREACHPortugueseportuguesereach
RMP BufferREACHGreekgreekreach
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