E.Z.N.A.® Plasmid DNA Midi Kit


  • Speed – Purification of plasmid DNA in < 60 minutes
  • Safety – No phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High quality – DNA is suitable for a variety of downstream applications

The E.Z.N.A. Plasmid Midi and Maxi Kits can isolate up to 250 μg and 1.2 mg of plasmid DNA from 50 mL and 200 mL bacterial cultures, respectively. These kits use a modified alkaline lysis method to lyse the cells and separate genomic DNA from plasmid DNA. Cellular debris are removed by centrifugation, and the protocol follows a simple bind, wash, and elute procedure to deliver high-quality

plasmid DNA. The system not only eliminates the time-consuming isopropanol precipitation step required by gravity flow columns but also the need for expensive accessories. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, ligation, PCR, in-vitro transcription, transformation, and other applications.

For Research Use Only. Not for use in diagnostic procedures.

Downstream ApplicationCloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material15-50 mL LB culture; Optimal Cell Mass (OD600 x mL culture) is 80-100.
Plasmid typeHigh-copy, low-copy, cosmid DNA
Processing modeManual (centrifugation or vacuum)
Throughput1 – 24
DNA binding technologySilica Midi Spin Column
Lysate clearance methodCentrifugation
Processing time<60 minutes
Yield100-250 µg for high copy-number; 10-50 µg for low copy-number
HiBind® DNA Mini ColumnsView Product
15 mL Collection Tubes
Solution IView Product
Solution IIView Product
Solution IIIView Product
HBC BufferView Product
DNA Wash BufferView Product
RNase AView Product
Elution BufferView Product

Table 1.  pGEM plasmid was purified from 50 mL DH5α cultures harboring the plasmid using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Midi Kit following manufacturer’s recommended protocol. Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system.

Product Information

Document NameLanguagesLink
E.Z.N.A.® Plasmid DNA Midi & Maxi KitsEnglish
Protocol ManualEnglish
Quick GuideEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
DNA Wash BufferGHSSpanishspanishghs
DNA Wash BufferGHSEnglishenglishghs
DNA Wash BufferWHMSFrenchfrenchwhms
DNA Wash BufferWHMSEnglishenglishwhms
DNA Wash BufferREACHSwedishswedishreach
DNA Wash BufferREACHSpanishspanishreach
DNA Wash BufferREACHNorwegiannorwegianreach
DNA Wash BufferREACHItalianitalianreach
DNA Wash BufferREACHGermangermanreach
DNA Wash BufferREACHFrenchfrenchreach
DNA Wash BufferREACHFinnishfinnishreach
DNA Wash BufferREACHDanishdanishreach
DNA Wash BufferREACHEnglishenglishreach
Elution BufferREACHGreekgreekreach
Elution BufferREACHPortugueseportuguesereach
Elution BufferREACHHungarianhungarianreach
Elution BufferREACHDutchdutchreach
Elution BufferWHMSFrenchfrenchwhms
Elution BufferWHMSEnglishenglishwhms
Elution BufferREACHSwedishswedishreach
Elution BufferREACHSpanishspanishreach
Elution BufferREACHItalianitalianreach
Elution BufferGHSEnglishenglishghs
Elution BufferGHSSpanishspanishghs
Elution BufferREACHEnglishenglishreach
Elution BufferREACHDanishdanishreach
Elution BufferREACHFrenchfrenchreach
Elution BufferREACHNorwegiannorwegianreach
Elution BufferREACHGermangermanreach
Elution BufferREACHFinnishfinnishreach
HBC BufferGHSEnglishenglishghs
HBC BufferWHMSFrenchfrenchwhms
HBC BufferWHMSEnglishenglishwhms
HBC BufferREACHSwedishswedishreach
HBC BufferREACHSpanishspanishreach
HBC BufferREACHNorwegiannorwegianreach
HBC BufferREACHGermangermanreach
HBC BufferGHSSpanishspanishghs
HBC BufferREACHEnglishenglishreach
HBC BufferREACHDanishdanishreach
HBC BufferREACHFrenchfrenchreach
HBC BufferREACHItalianitalianreach
HBC BufferREACHFinnishfinnishreach
RNase AGHSSpanishspanishghs
RNase AWHMSFrenchfrenchwhms
RNase AWHMSEnglishenglishwhms
RNase AREACHSwedishswedishreach
RNase AREACHSpanishspanishreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHGermangermanreach
RNase AGHSEnglishenglishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFrenchfrenchreach
RNase AREACHItalianitalianreach
RNase AREACHFinnishfinnishreach
Solution IGHSSpanishspanishghs
Solution IWHMSFrenchfrenchwhms
Solution IWHMSEnglishenglishwhms
Solution IREACHSwedishswedishreach
Solution IREACHSpanishspanishreach
Solution IREACHNorwegiannorwegianreach
Solution IREACHGermangermanreach
Solution IGHSEnglishenglishghs
Solution IREACHEnglishenglishreach
Solution IREACHDanishdanishreach
Solution IREACHFrenchfrenchreach
Solution IREACHItalianitalianreach
Solution IREACHFinnishfinnishreach
Solution IIREACHFinnishfinnishreach
Solution IIREACHNorwegiannorwegianreach
Solution IIREACHSwedishswedishreach
Solution IIREACHItalianitalianreach
Solution IIREACHDanishdanishreach
Solution IIREACHSpanishspanishreach
Solution IIGHSSpanishspanishghs
Solution IIREACHFrenchfrenchreach
Solution IIWHMSEnglishenglishwhms
Solution IIWHMSFrenchfrenchwhms
Solution IIREACHEnglishenglishreach
Solution IIGHSEnglishenglishghs
Solution IIREACHGermangermanreach
Solution IIIREACHEnglishenglishreach
Solution IIIREACHDanishdanishreach
Solution IIIREACHFinnishfinnishreach
Solution IIIREACHFrenchfrenchreach
Solution IIIREACHGermangermanreach
Solution IIIREACHItalianitalianreach
Solution IIIREACHNorwegiannorwegianreach
Solution IIIREACHSpanishspanishreach
Solution IIIREACHSwedishswedishreach
Solution IIIWHMSEnglishenglishwhms
Solution IIIWHMSFrenchfrenchwhms
Solution IIIGHSEnglishenglishghs
Solution IIIGHSSpanishspanishghs
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