Rare Variant Allele Detection from Urinary cfDNA by Droplet Digital PCR
Extraction of cfDNA from 4 mL preserved urine sample.
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Precision Medicine
An Automated, Turn-Key cfDNA Extraction Workflow Showcasing Low Frequency Variant Detection
Automated cell-free DNA extraction from 4 mL plasma.
Using ddPCR to Detect Rare Variant Alleles in cfDNA Samples on the MagBinder® Fit24 Platform
Carisa Townsend1, Kiranmai Durvasula1, Julie Baggs1, and Travis Butts1 Omega Bio-tek, Inc, Norcross, GA 30071 Introduction Recent advancements and studies have demonstrated the potential of cfDNA as a universal, non-invasive biomarker in cancer prognosis, diagnosis, and prenatal testing. cfDNA are found in low abundance and in a background of contaminating genomic DNA making it a
What are Biobanks?
In the era of precision medicine, biobanking has emerged as a crucial component in the realm of healthcare. A biobank is a specialized facility or repository that systematically collects, processes, stores, and manages biological samples and associated data for use in research and medical studies. The primary purpose of a biobank is to provide a
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The Challenges of cfDNA Purification
Cell-free DNA (cfDNA) has emerged as a revolutionary tool in the realm of molecular diagnostics, offering a non-invasive window into the genetic information of various bodily tissues. As discussed in a previous post, the development of cfDNA technology is a large step forward in detecting and profiling circulating biomarkers to derive clinically relevant information. However,
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What is PCR?
Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify and replicate specific segments of DNA. Over time, PCR technology has evolved, giving rise to various methodologies, each tailored to specific needs. In this blog post, we will explore the different types of PCR and their applications. Standard PCR Conventional PCR enables the
What is NGS?
Next-Generation Sequencing (NGS) is a cutting-edge technology used to sequence nucleotides in a DNA or RNA molecule. Unlike Sanger Sequencing, which can sequence only one DNA fragment at a time, NGS allows researchers to sequence multiple DNA fragments in parallel in a single run. This high-throughput approach revolutionizes our ability to decode complex genomes efficiently