Carisa Townsend1, Kiranmai Durvasula1, Julie Baggs1, and Travis Butts1 Omega Bio-tek, Inc, Norcross, GA 30071 Introduction Recent advancements and studies have demonstrated the potential of cfDNA as a universal, non-invasive biomarker in cancer prognosis, diagnosis, and prenatal testing. cfDNA are found in low abundance and in a background of contaminating genomic DNA making it a […]
Cell-free DNA (cfDNA) has emerged as a revolutionary tool in the realm of molecular diagnostics, offering a non-invasive window into the genetic information of various bodily tissues. As discussed in a previous post, the development of cfDNA technology is a large step forward in detecting and profiling circulating biomarkers to derive clinically relevant information. However,
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Next-Generation Sequencing (NGS) is a cutting-edge technology used to sequence nucleotides in a DNA or RNA molecule. Unlike Sanger Sequencing, which can sequence only one DNA fragment at a time, NGS allows researchers to sequence multiple DNA fragments in parallel in a single run. This high-throughput approach revolutionizes our ability to decode complex genomes efficiently
Revolutionary advancements in medical technologies have armed physicians with state-of-the-art methods for detecting and monitoring cancer. Two of these methods are liquid and solid biopsies which provide physicians and researchers with integral information about a patient’s condition. What is a Solid Biopsy? Solid biopsies are the traditional method of analyzing tumors; this typically involves extracting samples of tissue from the tumor using a needle or
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With advancements in purification protocols, laboratory equipment, and chemistry, it has become possible to isolate, quantify, and sequence cancer cell DNA and RNA efficiently. This is crucial for accurate diagnosis and helps support patient care-plan development, as it allows for a deeper understanding of the genetic make-up of the cancer cells and provides a roadmap
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Cell-free DNA (cfDNA) refers to small DNA fragments found in the bloodstream and other bodily fluids, such as spinal fluid and urine. These fragments are derived from various sources, including the breakdown of cells, the release of DNA from damaged or dying cells, and the shedding of DNA by normal cells. The presence of cfDNA
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Formalin-fixed paraffin-embedded (FFPE) tissue samples are commonly used for preserving and storing biological samples, such as cancer tumor samples, for future analysis. However, the formalin fixation and paraffin embedding process can significantly impact the quality and yield of extracted DNA, making it challenging for downstream applications such as PCR and NGS. Omega Bio-tek developed the Mag-Bind® FFPE
Research publication review and discussion: “Genome-wide cell-free DNA mutational integration enables ultra-sensitive cancer monitoring”, Nature Medicine. Circulating, cell-free DNA (cfDNA) holds great clinical significance for non-invasive disease detection, diagnosis, and monitoring for tumor, cancer, or fetal DNA studies. Circulating DNA extraction is often challenging as they are found in low quantities, and accurate, consistent methods
