Extraction of DNA and RNA from the sample source maximizes the information gained from precious or limited materials, providing an integrated molecular view. It enables researchers to correlate both genomic and transcriptomic data from the cellular matrix, eliminating the problem of sample variability. Omega Bio-tek has developed an innovative nucleic acid extraction methodology (Mag-Bind® DNA/RNA […]
Circulating free DNA (cfDNA) derived from urine has emerged as a promising biomarker in the field of non-invasive diagnostics, particularly in oncology, nephrology, and other areas of medical research. With advances in next-generation sequencing (NGS) and other molecular techniques, such as droplet digital PCR (ddPCR), the analysis of cfDNA from urine holds the potential to
Extraction of cfDNA from 4 mL preserved urine sample.
Rare Variant Allele Detection from Urinary cfDNA by Droplet Digital PCR Read More »
Automated cell-free DNA extraction from 4 mL plasma.
Carisa Townsend1, Kiranmai Durvasula1, Julie Baggs1, and Travis Butts1 Omega Bio-tek, Inc, Norcross, GA 30071 Introduction Recent advancements and studies have demonstrated the potential of cfDNA as a universal, non-invasive biomarker in cancer prognosis, diagnosis, and prenatal testing. cfDNA are found in low abundance and in a background of contaminating genomic DNA making it a
Cell-free DNA (cfDNA) has emerged as a revolutionary tool in the realm of molecular diagnostics, offering a non-invasive window into the genetic information of various bodily tissues. As discussed in a previous post, the development of cfDNA technology is a large step forward in detecting and profiling circulating biomarkers to derive clinically relevant information. However,
The Challenges of cfDNA Purification Read More »
Next-Generation Sequencing (NGS) is a cutting-edge technology used to sequence nucleotides in a DNA or RNA molecule. Unlike Sanger Sequencing, which can sequence only one DNA fragment at a time, NGS allows researchers to sequence multiple DNA fragments in parallel in a single run. This high-throughput approach revolutionizes our ability to decode complex genomes efficiently
Revolutionary advancements in medical technologies have armed physicians with state-of-the-art methods for detecting and monitoring cancer. Two of these methods are liquid and solid biopsies which provide physicians and researchers with integral information about a patient’s condition. What is a Solid Biopsy? Solid biopsies are the traditional method of analyzing tumors; this typically involves extracting samples of tissue from the tumor using a needle or
Liquid vs. Solid Biopsy Read More »
With advancements in purification protocols, laboratory equipment, and chemistry, it has become possible to isolate, quantify, and sequence cancer cell DNA and RNA efficiently. This is crucial for accurate diagnosis and helps support patient care-plan development, as it allows for a deeper understanding of the genetic make-up of the cancer cells and provides a roadmap
Why Purify Cancer DNA and RNA? Read More »
Cell-free DNA (cfDNA) refers to small DNA fragments found in the bloodstream and other bodily fluids, such as spinal fluid and urine. These fragments are derived from various sources, including the breakdown of cells, the release of DNA from damaged or dying cells, and the shedding of DNA by normal cells. The presence of cfDNA
What is cell-free DNA (cfDNA)? Read More »
